一种新型微生态保健品在兽医临床中的应用研究

利用一种微生态保健品和3种抗菌素药物，即氟哌酸、氯霉素、庆大霉素在兽医临床上用于治疗仔猪黄白痢，断奶猪的应激性腹泻，肥猪及母猪的腹泻作了对比试验，结果表明：微生态保健品对仔猪黄痢的治愈率为93％，对仔猪白痢的治愈率为95％，对断奶仔猪的应激性腹泻治愈率为94％，对肥猪及母猪的腹泻治愈率为100％。与氟哌酸、庆大霉素的疗效比较，差异显著（P＜0．05）；与氯霉素比较差异极显著（P＜0．01）．     

微生物谷氨酰胺转胺酶(MTG)分批发酵模型的建立

在本文中，以本实验优化好的工艺条件，建立了关于微生物谷氨酰胺转胺酶分批发酵的数学模型方程细胞生长的动力学方程、产物合成的动力学方程和底物消耗的动力学方程。并对所建立的动力学方程进行了生物统计分析，结果表明所建立的模型具有一定的可信度。     

植酸酶在饲料中的研究与应用

植酸酶在饲料中应用，不仅能提高植酸磷的消化率，减少配方中无机磷的添加量和磷排泄污染，还可改善和提高蛋白质、能量、氨基酸和微量元素的利用率。     

微生物燃料电池在环境监测中的研究进展

微生物燃料电池在环境监测领域有巨大的应用潜力.概述微生物燃料电池应用于环境监测的基本原理,对目前已有的微生物燃料电池型传感器为监测方法进行详细的介绍,包括微生物燃料电池用于易降解碳源、有毒污染物及微生物数量检测三方面的研究.最后探讨了微生物燃料电池传感器目前还未被广泛应用于实际水质监测的原因,以期为未来研究开发高性能微生物燃料电池传感器提供理论参考依据.     

T-RFLP指纹图谱技术分析细菌型微生物肥料菌种稳定性

[目的]建立一种分析微生物肥料菌种稳定性的T-RFLP指纹图谱技术。[方法]采用T-RFLP指纹图谱技术对进口细菌型微生物肥料菌种的稳定性进行分析。[结果]样品中优势菌的T-RFs片段主要是87、246、247、330 bp,其中330 bp为主要片段,Shannon多样性指数和均一性指数测定结果表明,不同批次产品间差异性较小,微生物群落结构较稳定。[结论]建立了微生物肥料菌种稳定性分析的T-RFLP指纹图谱技术,为进出口微生物肥料产品提供了一种快速分析方法。     

Sewage sludge processing determines its impact on soil microbial community structure and function

Composting and thermal drying are amongst the most commonly used post-digestion processes for allowing sanitation and biological stabilization of sewage sludge from municipal treatment plants, and making it suitable as soil conditioner for use in agriculture. To assess the impact of sludge-derived materials on soil microbial properties, fresh (LAF), composted (LAC) and thermally dried (LAT) sludge fractions, each resulting from a different post-treatment process of a same aerobically digested sewage sludge, were added at 1% (w/w) application rate on two contrasting (a loam and a loamy sand) soils and incubated under laboratory conditions for 28 days. Soil respiration, microbial ATP content, hydrolytic activities and arginine ammonification rate were monitored throughout the incubation period. Results showed that soil biochemical variables, including the metabolic quotient (qCO₂), were markedly stimulated after sludge application, and the magnitude of this stimulatory effect was dependent on sludge type (precisely LAT > LAF > LAC), but not on soil type. This effect was related to the content of stable organic matter, which was lower in LAT. Genetic fingerprinting by PCR–DGGE revealed that compositional shifts of soil bacterial and, at greater extent, actinobacterial communities were responsive to the amendment with a differing sludge fraction. The observed time-dependent changes in the DGGE profiles of amended soils reflected the microbial turnover dependent on the sludge nutrient input, whereas no indications of adverse effects of sludge-borne contaminants were noted. Our findings indicate that composting rather thermal drying can represent a more appropriate post-digestion process to make sewage sludge suitable for use as soil conditioner in agriculture.     

Earthworms increase soil microbial biomass carrying capacity and nitrogen retention in northern hardwood forests

Earthworms have been shown to produce contrasting effects on soil carbon (C) and nitrogen (N) pools and dynamics. We measured soil C and N pools and processes and traced the flow of ¹³C and ¹⁵N from sugar maple (Acer saccharum Marsh.) litter into soil microbial biomass and respirable C and mineralizable and inorganic N pools in mature northern hardwood forest plots with variable earthworm communities. Previous studies have shown that plots dominated by either Lumbricus rubellus or Lumbricus terrestris have markedly lower total soil C than uncolonized plots. Here we show that total soil N pools in earthworm colonized plots were reduced much less than C, but significantly so in plots dominated by contain L. rubellus. Pools of microbial biomass C and N were higher in earthworm-colonized (especially those dominated by L. rubellus) plots and more ¹³C and ¹⁵N were recovered in microbial biomass and less was recovered in mineralizable and inorganic N pools in these plots. These plots also had lower rates of potential net N mineralization and nitrification than uncolonized reference plots. These results suggest that earthworm stimulation of microbial biomass and activity underlie depletion of soil C and retention and maintenance of soil N pools, at least in northern hardwood forests. Earthworms increase the carrying capacity of soil for microbial biomass and facilitate the flow of N from litter into stable soil organic matter. However, declines in soil C and C:N ratio may increase the potential for hydrologic and gaseous losses in earthworm-colonized sites under changing environmental conditions.     

Soil aggregation: Influence on microbial biomass and implications for biological processes

Our 1988 paper, describing the effects of cultivation on microbial biomass and activity in different aggregate size classes, brought together the ‘aggregate hierarchy theory’ and the ‘microbial biomass concept’. This enabled us to identify the relationships between microbial and microhabitat (aggregate) properties and organic matter distribution and explain some of their responses to disturbance. By combining biochemical and direct microscopy based quantification of microbial abundance with enzyme activities and process measurements, this study provided evidence for the role of microbial biomass (especially fungi) in macroaggregate dynamics and carbon and nutrient flush following cultivation. In the last ten years environmental genomic techniques have provided much new knowledge on bacterial composition in aggregate size fractions yet detailed information about other microbial groups (e.g. fungi, archaea and protozoa) is lacking.We now know that soil aggregates are dynamic entities – constantly changing with regard to their biological, chemical and physical properties and, in particular, their influences on plant nutrition and health. As a consequence, elucidation of the many mechanisms regulating soil C and nutrient dynamics demands a better understanding of the role of specific members of microbial communities and their metabolic capabilities as well as their location within the soil matrix (e.g. aggregates, pore spaces) and their reciprocal relationship with plant roots. In addition, the impacts of environment and soil type needs to be quantified at the microscale using, wherever possible, non-destructive ‘in situ’ techniques to predict and quantify the impacts of anthropogenic activities on soil microbial diversity and ecosystem level functions.