大豆不育系传粉昆虫及传粉技术研究

报道了公主岭地区大豆野生传粉昆虫种类及筛选出的苜蓿切叶蜂为大豆不育系在网室内和田间的传粉昆虫 ,使网室内不育系结实率平均达到了 70 %以上 ,田间达到了 75 %以上 ,并讨论了提高传粉效率的途径和方法。     

Effects of leguminous plant residues and NPK fertilizer application on the performance of yam (Dioscorea rotundata ‘c.v.’ ewuru) in south-western Nigeria

The effects of cultivating and incorporating residues of previous tropical kudzu (Pueraria phaseoloides) and soybean (Glycine max) with application of NPK fertilizer on yam performance were evaluated at the teaching and research farm, LAUTECH, Nigeria. There were nine treatments: incorporation of legume residues (5 t DM ha^?1), application of recommended fertilizer rate for yam (90–50–75 kg NPK ha^?1) in the zone or 50% of recommended rate (45–25–37.5 kg NPK ha^?1), alone and in combination with residues and a control without residues or fertilizer in a randomized complete block design. Cultivation of previous legumes reduced soil nematode population (>200%) compared with no legumes. For both years, application of Pueraria residues improved tuber yield by an average of 15.8% compared with control. Fertilizer application enhanced arbuscular mycorrhizal (AM) colonization of yam roots but AM colonization was lower (～50%) in plots where Pueraria residues were incorporated compared with other plots. Combined application of plant residues with fertilizer improved soil organic carbon, total N, exchangeable Ca and Mg compared with application of NPK fertilizer. From these results, it is concluded that half of the recommended NPK rate may be adequate and incorporation of residues with reduced NPK fertilizer application may be a sustainable soil fertility management option for continuous yam production.     

Estimation of hybrid seed production and efficient pollen flow using insect pollination of male sterile soybeans in caged plots

The aptitude of leafcutter bees to pollinate male sterile soybean plants (ms2 gene) in caged plots was evaluated in four experiments from both quantitative and qualitative points of view. The m.s. plant seed set was satisfactory: on average, it represented 60% of the male fertile isogenic seed set (with a range between 44 and 69%). The lower yield of m.s. plants was linked with a smaller number of fertile reproductive nodes. The efficient pollen flow was observed over the flowering period with both morphological and electrophoretic markers. Insect behaviour was not influenced by flower colour. Differences between flowering duration of pollen donors appear to be the major factor inducing unbalanced populations. The interest of this technique as a tool for dynamic management of the genetic variation in populations or for hybrid seed production is discussed.     

Responses of white yam (Dioscorea rotundata) cultivars to inoculation with three viruses

The responses of 24 white yam ( Dioscorea rotundata ) cultivars to mechanical and vector transmission with each of three viruses infecting yams were assessed through enzyme-linked immunosorbent assay (ELISA) and symptom development. The viruses were Dioscorea alata virus (DAV), genus Potyvirus ; Dioscorea alata bacilliform virus (DaBV), genus Badnavirus ; and Cucumber mosaic virus (CMV), genus Cucumovirus . Only TDr 95-128, a landrace cultivar from Nigeria, developed symptoms of infection with CMV and DaBV following mechanical and vector transmission, respectively. PAS-ELISA showed that nine genotypes remained uninfected by DAV and 11 were uninfected by CMV or DaBV. Genotypes TDr 747 and TDr 1640 both showed resistance to all three viruses.     

不同化性苜蓿切叶蜂(Megachile rotundata F.)成蜂对白僵菌敏感性的差异

室内测定了不同化性苜蓿切叶蜂成蜂对球孢白僵菌(Beauveria bassiana(Balsamo))的敏感性。在28℃,RH60%的条件下,喷施白僵菌后二化种群死亡速度较快,72h后死亡率为67%,而一化种群72h后死亡率为38%。结果表明,成蜂对白僵菌的敏感性在化性间存在差异,二化种群成蜂对球孢白僵菌具有较高的敏感性(P<0.05),抗性较差。     

北方切叶蜂的研究初报

首次报道了吉林省公主岭地区野生传粉昆虫———北方切叶蜂 (Megachilemanchuri anaYasumatsu)的形态特征、生物学特性、传粉行为以及影响种群数量变动的主要因子     

Detection and diversity of viruses infecting African yam (Dioscorea rotundata) in a collection and F1 progenies in Côte d'Ivoire shed light to plant-to-plant viral transmission

Yam (Dioscorea spp.) is a major staple food whose production is hampered by viral diseases. However, the prevalence, diversity, transmission, and impact of yam-infecting viruses remain poorly documented. This study reports on the symptomatology, prevalence, and molecular diversity of eight viruses in 38 D. rotundata accessions from a germplasm collection and 206 F₁ hybrid progenies maintained in Côte d'Ivoire. Mean severity scores as assessed from leaf symptoms ranged from 2 to 4 in the germplasm collection and from 1 to 3 in F₁ hybrids, respectively. Dioscorea mosaic-associated virus (DMaV), potexviruses, and yam mosaic virus (YMV) were detected by PCR-based diagnosis tools in single and mixed infections in both the D. rotundata collection and F₁ progenies, whereas badnaviruses were detected only in the germplasm collection. In contrast, cucumber mosaic virus (CMV), yam macluraviruses, yam asymptomatic virus 1 (YaV1), and yam mild mosaic virus (YMMV) could not be detected. No correlation could be established between severity scores and indexing results. Phylogenetic analysis performed on partial viral sequences amplified from infected samples unveiled the presence of two putative novel viral species belonging to genera Badnavirus and Potexvirus and provided evidence for plant-to-plant transmission of YMV, DMaV, and yam potexviruses.     

苜蓿切叶蜂(Megachile rotundata F.)雄蜂对大豆不育系结实率的影响

报道了苜蓿切叶蜂雄蜂在网室内对大豆不育系授粉的效果。结果表明,尽管雄蜂的传粉效率显著低于雌蜂,但雄蜂在网室内的传粉效果还是非常显著的。释放雄蜂网室内大豆不育系JLCMS8A和JLCMS17A的平均单株结荚数和粒数分别为10.3个、16.6粒和37.8个、84.3粒,分别是释放雌蜂后结荚率和结实率的35.0%、34.9%和77.5%、70.2%。过去数年的研究结果表明,所有不育系在网室内没有传粉昆虫时,结荚率和结实率均低于3%,表明苜蓿切叶蜂雄蜂在网室内是大豆不育系有效的传粉昆虫。     

苜蓿切叶蜂(Megachile rotunclata F.)雄蜂对大豆不育系结实率的影响

报道了苜蓿切叶蜂雄蜂在网室内对大豆不育系授粉的效果.结果表明,尽管雄蜂的传粉效率显著低于雌蜂,但雄蜂在网室内的传粉效果还是非常显著的.释放雄蜂网室内大豆不育系JLCMS8A和JLCMS17A的平均单株结荚数和粒数分别为10.3个、16.6粒和37.8个、84.3粒,分别是释放雌蜂后结荚率和结实率的35.0%、34.9%和77.5%、70.2%.过去数年的研究结果表明,所有不育系在网室内没有传粉昆虫时,结荚率和结实率均低于3%,表明苜蓿切叶蜂雄蜂在网室内是大豆不育系有效的传粉昆虫.