检测砂梨潜隐病毒的IC-RT-PCR和TC-RT-PCR的研究

以砂梨为试材,在生物学和血清学检测的基础上,采用免疫捕作RT-PCR穴IC-RT-PCR雪和试管捕作RT-PCR穴TC-RT-PCR雪技术,对苹果褪绿叶斑病毒(Applechloroticleafspotvirus,ACLSV)和苹果茎沟病毒(Applestemgroovingvirus,ASGV)进行了检测分析。结果表明,TC/IC-RT-PCR均能有效检测梨粗提液中的ACLSV和ASGV,分别获得了大小约358bp和499bp的目标扩增片段;但IC-RT-PCR检测ACLSV的效果受到病毒分离株间血清学关系影响。与传统的RT-PCR相比,IC/TC-RT-PCR不仅灵敏度更高,而且不需提取总RNA,可以简化操作程序和减少苯酚、氯仿类有机试剂对人体的伤害和对环境的污染,适合于大量梨样品的病毒快速灵敏检测。     

Simple diagnosis using ethanol immersion of strawberry plants with latent infection by Colletotrichum acutatum, Dendrophoma obscurans, and Fusarium oxysporum f. sp. fragariae

Simple diagnosis by ethanol immersion (SDEI) to detect Glomerella cingulata was used to detect three other fungi that also cause latent infection of strawberry plants. Signs on strawberry leaves with asymptomatic latent infection by Colletotrichum acutatum became visible using SDEI. Salmon-pink conidial masses were produced in the acervuli on the treated leaves 5 days after incubation at 28°C. In the case of Dendrophoma obscurans, pycnidia with amber conidial masses formed 5 days after incubation at 28°C. The pycnidia were observed mainly on the ribs, and conidial masses exuded from the ostiole. These macroscopic conidial masses were similar to those of G. cingulata and C. acutatum. When water was dripped onto a lesion caused by D. obscurans, the pycnidia exuded white filamentous conidial masses, making the distinction of D. obscurans from G. cingulata or C. acutatum. On petioles with latent infection by Fusarium oxysporum f. sp. fragariae, white aerial hyphae grew out from the vascular tissues on the cut surface 3 days after incubation at 28°C and were easily observed by eye or with a loupe. Thus, SDEI was also useful for diagnosing latent infection of strawberry plants by C. acutatum, D. obscurans, and F. oxysporum f. sp. fragariae.     

劳动力老龄化对我国农业生产效率的影响

针对我国农村人口老龄化现象不断加剧,致使农业劳动力供给质量下降等问题,以2009年农村固定观察点数据为依据,分析我国农业劳动力老龄化现状,并利用柯布道格拉斯生产函数研究劳动力老龄化对农业生产效率的影响。结果表明:我国农业生产中存在严重的老龄化现象,60岁以上的劳动力占18.42%;与年轻劳动力相比,老龄劳动力的生产效率明显较低,老龄农户不仅主要生产要素的边际产值低于非老龄农户,而且耕种面积以及其他各生产要素的投入水平也均低于非老龄户,这说明农业老龄化严重制约着我国农业的发展。     

《侵权责任法》背景下的劳动关系辨析

企业未办理营业执照就进行生产，工人工作时受伤，本应按照《工伤保险条例》进行赔偿，适用无过错原则。然而，一些基层法院认为，双方形成了个人之间的劳务关系，应按《侵权责任法》的规定，适用过错相抵原则，这严重损害了劳动者的合法权益。劳动关系与劳务关系相比较，劳动关系具有产业性、劳动者的从属性、生产的组织性和期限的长期性等特征。通过对劳动关系内涵的分析，有助于我们正确处理案件，就不会错误地将本是劳动关系的案件认定为双方存在劳务关系，也不会将工伤案件按劳务关系处理。     

人口老龄化对中国劳动力供给的影响分析

通过对中国人口统计的一些数据分析,研究了人口老龄化对中国劳动力供给的影响.研究结果表明,老龄化造成劳动力供给数量减少,同时老龄化影响中国劳动力供给质量.可采取应对措施:一是提高科技水平和人口素质,二是充分开发和利用老年人力资本.     

The Reformation of the Construction Employment System and Building a Harmonious Society

Construction industry is not only the pillar industry in national economy,but also the typical industry with intensive labors.As a major part of the construction industry,the Construction Employment System(CES) is an important factor of the market order,as well as the quality,the safety and the development of the industry.It is also an important part for building a harmonious society.It concerns the social harmony,political harmony,societal harmony,cultural harmony,urban-rural harmony,etc.However,at present,there are still many problems in CES that might influence the harmonious society building.This paper advises to speed up the reformation of CES,to establish and consummate the system of construction labor subcontract,to develop vigorously labor subcontract corporations,to improve the quality of the labors,to ensure the rights and interests of the labor.     

对三农问题和农村城市化问题的若干思考

中国是一个农业大国，农业经济状况在很大程度上决定了整体经济的发展态势。对中国当前农业发展现状中的主要问题进行分析，认为只有积极进行城市化建设才能解决中国农业发展的根本问题，同时，就中国城市化的路径选择与城市化发展的中短期目标提出自己的看法。     

农民工社会保障相对独立性初探

以农民工社会保障体系与城镇、农村社会保障体系的关系为研究视角,提出了在现阶段农民工社会保障应相对独立于城镇社会保障体系和农村社会保障体系,这是农民工职业、身份、风险的变化的要求,是农民工社会保障不宜纳入城镇社会保障体系的现实选择。在此基础上,本文还提出建立相对独立的农民工社会保障体系的基本框架:即以工伤保险为先,医疗保险、失业保险、养老保险、最低保障为主,内容逐步扩充。     

不同砧木对葡萄新梢省力化特性的影响

通过对不同砧穗组合的新梢生长量、新梢生长速率、节间长度、基部粗度及副梢萌发率的测定,研究不同砧木对‘夏黑’和‘87-1’葡萄新梢省力化特性的影响。并调查各个砧穗组合的结果系数,研究省力化特性参数与结果系数的关系。然后运用Topsis评价法对各个砧穗组合进行综合评价。结果表明,对于‘夏黑’葡萄来说,夏黑/华葡1号组合省力化特性最好,结果系数最大,夏黑/抗砧1号组合次之,夏黑/140Ru组合省力化特性最差,结果系数最小。对于‘87-1’葡萄而言,87-1/华葡1号省力化特性最好,结果系数最大,87-1/5C组合省力化特性最差,87-1/ 140Ru结果系数最小。综上,华葡1号与抗砧1号可以有效降低‘夏黑’和‘87-1’葡萄的新梢生长量及副梢萌发率,提高结果系数,控制营养生长,促进生殖生长。