全文获取类型
收费全文 | 227篇 |
免费 | 12篇 |
国内免费 | 26篇 |
专业分类
林业 | 4篇 |
农学 | 26篇 |
基础科学 | 3篇 |
12篇 | |
综合类 | 111篇 |
农作物 | 32篇 |
水产渔业 | 12篇 |
畜牧兽医 | 30篇 |
园艺 | 22篇 |
植物保护 | 13篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2022年 | 6篇 |
2021年 | 10篇 |
2020年 | 6篇 |
2019年 | 12篇 |
2018年 | 12篇 |
2017年 | 16篇 |
2016年 | 14篇 |
2015年 | 12篇 |
2014年 | 6篇 |
2013年 | 15篇 |
2012年 | 26篇 |
2011年 | 14篇 |
2010年 | 21篇 |
2009年 | 17篇 |
2008年 | 13篇 |
2007年 | 18篇 |
2006年 | 7篇 |
2005年 | 9篇 |
2004年 | 5篇 |
2003年 | 4篇 |
2002年 | 3篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1997年 | 4篇 |
1996年 | 1篇 |
1988年 | 1篇 |
1981年 | 1篇 |
1962年 | 1篇 |
排序方式: 共有265条查询结果,搜索用时 0 毫秒
1.
2.
猪2型圆环病毒核酸疫苗免疫效应研究 总被引:9,自引:0,他引:9
PCR扩增PCV2 ORF2和BVP22基因。将ORF2基因克隆入真核表达载体pCDNA3.1(+),构建了pCORF2作为核酸疫苗免疫小白鼠。同时将具有蛋白转导功能的BVP22基因分别克隆到ORF2基因的上游和下游,使二者融合表达,命名为pCORF2BVP22和pCBVP220RF2。分4组肌肉免疫BALB/c小白鼠,分别注射pCDNA3.1(+)、pCORF2、pCORF2BVP22和pCBVP220RF2,共免疫2次,间隔2周,分别于首免后2周和二免后4周采血,ELISA法检测体液抗体。同时为在体外验证ORF2和BVP22基因的真核表达,将ORF2和BVP22基因分别克隆入pEGFP-N1载体,构建了pNORF2和pNBVP22,转染Hela细胞后在荧光显微镜下观察到了ORF2和BVP22在体外的瞬时高效表达。结果显示,通过两次免疫后,各疫苗组均产生了针对ORF2的体液抗体,同时证明BVP22可增强核酸疫苗的免疫效果,其中以BVP22在ORF2上游效果更好。本研究为防制猪2型圆环病毒感染提供了较为理想的侯选疫苗。 相似文献
3.
4.
Marine Remize Frdric Planchon Matthieu Garnier Ai Ning Loh Fabienne Le Grand Antoine Bideau Christophe Lambert Rudolph Corvaisier Aswani Volety Philippe Soudant 《Marine drugs》2022,20(1)
The production of polyunsaturated fatty acids (PUFA) in Tisochrysis lutea was studied using the gradual incorporation of a 13C-enriched isotopic marker, 13CO2, for 24 h during the exponential growth of the algae. The 13C enrichment of eleven fatty acids was followed to understand the synthetic pathways the most likely to form the essential polyunsaturated fatty acids 20:5n-3 (EPA) and 22:6n-3 (DHA) in T. lutea. The fatty acids 16:0, 18:1n-9 + 18:3n-3, 18:2n-6, and 22:5n-6 were the most enriched in 13C. On the contrary, 18:4n-3 and 18:5n-3 were the least enriched in 13C after long chain polyunsaturated fatty acids such as 20:5n-3 or 22:5n-3. The algae appeared to use different routes in parallel to form its polyunsaturated fatty acids. The use of the PKS pathway was hypothesized for polyunsaturated fatty acids with n-6 configuration (such as 22:5n-6) but might also exist for n-3 PUFA (especially 20:5n-3). With regard to the conventional n-3 PUFA pathway, Δ6 desaturation of 18:3n-3 appeared to be the most limiting step for T. lutea, “stopping” at the synthesis of 18:4n-3 and 18:5n-3. These two fatty acids were hypothesized to not undergo any further reaction of elongation and desaturation after being formed and were therefore considered “end-products”. To circumvent this limiting synthetic route, Tisochrysis lutea seemed to have developed an alternative route via Δ8 desaturation to produce longer chain fatty acids such as 20:5n-3 and 22:5n-3. 22:6n-3 presented a lower enrichment and appeared to be produced by a combination of different pathways: the conventional n-3 PUFA pathway by desaturation of 22:5n-3, the alternative route of ω-3 desaturase using 22:5n-6 as precursor, and possibly the PKS pathway. In this study, PKS synthesis looked particularly effective for producing long chain polyunsaturated fatty acids. The rate of enrichment of these compounds hypothetically synthesized by PKS is remarkably fast, making undetectable the 13C incorporation into their precursors. Finally, we identified a protein cluster gathering PKS sequences of proteins that are hypothesized allowing n-3 PUFA synthesis. 相似文献
5.
绥农22是黑龙江省农科院绥化农科所经有性杂交育成的高产抗病大豆新品种.于2005年2月经黑龙江省农作物品种审定委员会审定推广.该品种茎秆强硬抗倒,抗病,节短荚密,通风透光性好.蛋白质含量39.66%,脂肪含量20.06%.生育日数118 d左右,需活动积温2 400℃左右.2000年所内鉴定试验平均产量3 894.7 kg/hm2,比合丰25增产17.7%.2004年全省6点生产试验平均产量2 426.1 kg/hm2,比对照品种绥农14增产12%.适应黑龙江省第二积温带广大地区种植,以及吉林省和内蒙古自治区相适应的积温区种植.适宜5月上旬播种,该品种最适宜垄作窄行密植栽培,适宜密度保苗30~35万株/hm2,施种肥200 kg/hm2左右. 相似文献
6.
追施氮肥对中筋小麦小偃22产量和品质的影响 总被引:3,自引:0,他引:3
【目的】探讨追施氮肥对中筋小麦小偃22产量和品质的影响,为关中主栽小麦品种小偃22高产调优栽培措施的制定提供理论依据。【方法】2005~2006年以小麦品种小偃22为材料,于拔节期和孕穗期追施不同量的氮肥,检测其产量、籽粒品质指标和面粉粉质指标的变化。【结果】不同生育期追施氮肥后小偃22平均单产较对照提高13.6%,其中以拔节期施纯氮37.5 kg/hm2+孕穗期施纯氮75 kg/hm2处理增产效果最为显著,较对照净增1 350.5 kg/hm2,增幅达20.8%。追施氮肥处理平均籽粒蛋白质、湿面筋、干面筋含量及出粉率和吸水率显著或极显著提高,依次较对照提高6.6%,9.2%,9.9%,2.3%和2.3%;追氮对沉淀值和稳定时间影响不大,但平均硬度和弱化度较对照分别提高1.8%和22.4%;形成时间、评价值和粉质质量依次较对照降低8.3%,10.5%和16.8%。【结论】追施氮肥可显著提高小偃22的产量,显著改善其籽粒品质,但不利于粉质品质的改善。拔节期37.5 kg/hm2+孕穗期75 kg/hm2追氮组合对产量和籽粒的蛋白质品质及粉质品质效应最佳。 相似文献
7.
8.
9.
10.
Kana Naito Yasuhiro Ishiga Kazuhiro Toyoda Tomonori Shiraishi Yuki Ichinose 《Journal of General Plant Pathology》2007,73(4):281-285
Flagellin in Pseudomonas syringae is a potent elicitor of defense responses including hypersensitive cell death in dicot plants. The oligopeptides flg22 consisting
of 22 conserved amino acids near the N-terminus of flagellins is reported to induce plant defense responses. Because glycosylation
of the central domain of flagellin affects its elicitor activity, we investigated whether any peptide sequence in addition
to flg22 is required for flagellin-induced hypersensitive reaction. A study of recombinant flagellin polypeptides indicated
that the N-terminal domain including the conserved flg22 is required for flagellin-induced hypersensitive cell death in Arabidopsis thaliana. 相似文献