铁钉菜乙醇提取物对真菌生长的影响

铁钉菜乙醇粗提物通过薄层层析法分离活性物质,分别采用纸片法和菌饼法测定了铁钉菜提取物及薄层层析带对3种真菌的菌丝生长的影响,用玻片法测定了对孢子萌发的抑制作用。实验结果表明:乙醇提取物对3种真菌孢子萌发的抑制作用最强,其中,浓度在0.005 mg/mL时对绿色木霉和黑曲霉孢子萌发的抑制率均达到99%。     

Ishophloroglucin A,Isolated from Ishige okamurae,Alleviates Dexamethasone-Induced Muscle Atrophy through Muscle Protein Metabolism In Vivo

The in vitro capacity of Ishige okamurae extract (IO) to improve impaired muscle function has been previously examined. However, the mechanism underlying IO-mediated muscle protein metabolism and the role of its component, Ishophloroglucin A (IPA), in mice with dexamethasone (Dexa)-induced muscle atrophy remains unknown. In the present study, we evaluated the effect of IO and IPA supplementation on Dexa-induced muscle atrophy by assessing muscle protein metabolism in gastrocnemius and soleus muscles of mice. IO and IPA supplementation improved the Dexa-induced decrease in muscle weight and width, leading to enhanced grip strength. In addition, IO and IPA supplementation regulated impaired protein synthesis (PI3K and Akt) or degradation (muscle-specific ubiquitin ligase muscle RING finger and atrogin-1) by modulating mRNA levels in gastrocnemius and soleus muscles. Additionally, IO and IPA upregulated mRNA levels associated with muscle growth activation (transient receptor potential vanilloid type 4 and adenosine A1 receptor) or inhibition (myostatin and sirtuin 1) in gastrocnemius and soleus muscle tissues of Dexa-induced mice. Collectively, these results suggest that IO and IO-derived IPA can regulate muscle growth through muscle protein metabolism in Dexa-induced muscle atrophy.     

铁钉菜甲醇提取物对酪氨酸酶活性的抑制作用

为了研究海藻铁钉菜提取物的酪氨酸酶抑制活性,采用多巴速率氧化法测定不同浓度的铁钉菜甲醇提取物对酪氨酸酶活性的抑制率,通过酶促动力学方法与绘制Lineweaver-Burk 曲线推断其抑制类型;福林酚法分析其总酚含量、苯酚硫酸法测定其多糖含量、AlCl3显色法测定其总黄酮含量。结果表明：铁钉菜甲醇提取物能显著抑制酪氨酸酶活性,其半效应浓度IC50为1.823 g/L,抑制类型为非竞争型抑制;化学分析发现该甲醇提取物具有较高含量的总酚、多糖与总黄酮,含量分别为(51.37±2.77)、(35.92±5.27)、(43.16±0.33) mg/g。     

The Promoting Effect of Ishige sinicola on Hair Growth

This study was conducted to evaluate the promoting effect of Ishige sinicola, an alga native to Jeju Island, Korea, on hair growth. When vibrissa follicles were cultured in the presence of I. sinicola extract for 21 days, I. sinicola extract increased hair-fiber length. After topical application of I. sinicola extract onto the back of C57BL/6 mice, anagen progression of the hair shaft was induced. The I. sinicola extract significantly inhibited the activity of 5α-reductase. Treatment of immortalized vibrissa dermal papilla cells (DPCs) with I. sinicola extract resulted in increase of cell proliferation, which was accompanied by the increase of phospho-GSK3β level, β-catenin, Cyclin E and CDK2, whereas p27^kip1 was down-regulated. In particular, octaphlorethol A, an isolated component from the I. sinicola extract, inhibited the activity of 5α-reductase and increased the proliferation of DPCs. These results suggest that I. sinicola extract and octaphlorethol A, a principal of I. sinicola, have the potential to treat alopecia via the proliferation of DPCs followed by the activation of β-catenin pathway, and the 5α-reductase inhibition.