农业信息：传统与现代的耦合

近年来，国家投资建设大量信息基础设施，以保证农业信息能更好的被农户采用。而现实中，农户并不通过网络、电视和报刊等现代信息手段获得农业信息，相反，基于地缘和血缘的熟人网络和以盈利为目的的商人成了农业信息传播的主要媒介。在这种传播方式下，农户的利益常遭侵害，农业科技难以广泛有效的传播，甚至一些假信息让农户失去了对“科技”的信赖。针对目前存在的种种问题，笔者兼顾现实与未来，提出了相关对策。     

深化馆系资料室共建-扬馆系所长

本文分析了系部资料室在服务教学科研工作中的特殊地位与优势，同时分析了系部资料室建设存在的一些问题，提出系部资料室信息深化服务的建议。     

GIS在生态土地分类中的作用

This paper depicted the physiographic landscape features and natural vegetation situation of study area (the eastern Jilin Province), and expatiates the definition, basic characters and its development of Ecological Land Classification (ELC). Based on the combination of relief map, satellite photography for study area and vegetation inventory data of 480 sample sites, a 5-class and a 15-class ecological land type map was concluded according to 4 important factors including slope, aspect, vegetation and elevation. Ecological Classification System (ECS) is a method to identify, characterize, and map ecosystems. The Ecological Land Type (ELT) was examined and applied initially in eastern Jilin Province. Foundation item: This paper was supported by Chinese Academy of Sciences “100 people” project and the Open Research Station of Changbai Mountain Forest Ecosystem. Biography: XIAO Bao-ying (1974-), female, postgraduate in Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. China Responsible editor: Zhu Hong     

目前农业信息化发展面临的问题与几点建议

本文探讨了农业信息服务特点及开展与推广所面临的主要问题，对完善农业信息服务、实现农业信息化提出相关建议。     

我国农村信息化建设存在的问题及对策研究

农村信息化建设是国家信息化战略的重要组成部分，是实现小康社会的重要途径。我国的农村信息化建设取得了初步的成绩，但还存在许多问题，针对这些问题本文提出了具体的应对策略。     

景观植物信息查询系统(LPIIS)的构建

近年来，我国的景观植物产业得到了很大发展，但仍存在很多问题，为了解决这些问题，我们开发了景观植物信息查询系统(简称LPIIS)。LPIIS的构建包括后台数据库和管理系统2部分，以Microsoft Access开发后台景观植物数据库，实现各种零散的景观植物数据资料的数字化；采用面向对象的开发语言VB进行信息系统的界面设计和系统集成，实现了人性化的界面，操作更为方便。LPIIS的出现改变了过去景观植物信息管理的手工方式，具有可视化人性化的界面、提供多媒体信息、数据的维护和更新具有继承性、后台数据库可方便的移植于网络等特点。     

Effect of cGMP on voltage-gated potassium channel in pulmonary artery smooth muscle cells from rats exposed to chronic hypoxia

AIM: To investigate the effect of cGMP on voltage-gated potassium channel in pulmonary artery smooth muscle cells (PASMCs) from rats exposed to chronic hypoxia. METHODS: (1) Wistar rats were randomly divided into control group (group A) and chronic hypoxia group (group B). Then group B received hypoxia 8 hours per day for 4 consecutive weeks. (2) Single PASMC was obtained via acute enzyme separation method. (3) Conventional whole-cell patch clamp technique was used to record resting membrane potential (Em) and ion currents of voltage-gated potassium channel. The changes of ion currents of voltage-gated potassium channel before and after applying cGMP (1 mmol/L), an agonist of protein kinase G (PKG), and cGMP plus H-8 (1 mmol/L), an inhibitor of PKG were compared between two groups. RESULTS: The Em of group B were significantly lower than that of group A. The ion currents of voltage-gated potassium channel in group A and group B were all significantly inhibited by cGMP [control group: from (118.0±5.0) pA/pF to (89.9±16.5) pA/pF, n=6, P＜0.05;chronic hypoxia group: from (81.0±5.0) pA/pF to (56.8±9.1) pA/pF, n=6, P＜0.05]and these effects were reversed by H-8 [control group: from (119.2±10.3) pA/pF to (117.8±9.1) pA/pF, n=6, P＞0.05;chronic hypoxia group: from (96.8±6.2)　pA/pF to (98.0±2.2)　pA/pF, n=6, P＞0.05]. CONCLUSIONS: The currents of voltage-gated potassium channel was inhibited by chronic hypoxic. The inhibitory effect of cGMP on currents of voltage-gated potassium channel in PASMCs from both normal and chronic hypoxic rats may be probably through the phosphorylation of voltage-gated potassium channel.     

Changes of K+ channels of outer hair cells in guinea pig cochlea with streptomycin ototoxicity

AIM:To study the changes of K⁺ channels of outer hair cells in guinea pig cochlea with streptomycin ototoxicity. METHODS:Auditory brainstem responses (ABR) and whole-cell patch clamp techniques were used.RESULTS:(1) The body weight of guinea pigs with streptomycin ototoxicity decreased significantly; (2) The ABR threshold markedly increased in streptomycin group (Ⅱ,Ⅲ);(3)The number of dissociated outer hair cells of guinea pigs (Ⅱ,Ⅲ) was lower than that of control (Ⅰ); (4) Streptomycin decreased the Ca²⁺-sensitive K⁺ currents and delayed outward K⁺ currents distinctly; (5) There was no significant difference of K⁺ currents between Ⅰ and Ⅱ/Ⅲ. CONCLUSION:These results suggest that the inhibition of K⁺ channels is the basis of streptomycin ototoxicity, but not the direct reason for cell death.     

Role of potassium channels in the regulation of intracellular free Ca2+ concentration of pulmonary artery smooth muscle cells in rats

AIM: To investigate the role of potassium channels in the regulation of intracellular free calcium concentration ( [Ca²⁺]_i) of pulmonary artery smooth muscle cells (PASMCs) in rats. METHODS: The fluorescence Ca²⁺ indicator Fura-2/AM was used to observe [Ca²⁺]_i of rat PASMCs in normal and chronic hypoxic condition. The influences of potassium channels on PASMCs proliferation were assessed by MTT assay. RESULTS: 1. In normoxic condition, [Ca²⁺]_i was (156.91±8.60) nmol/L, and in hypoxic condition, [Ca²⁺]_i was (294.01±16.81) nmol/L. 2. In normoxic condition, the voltage-dependent K⁺-channel antagonist 4-aminopyridine (4AP), but not the Ca²⁺-activated K⁺-channel antagonist tetraethylammonium (TEA) and the ATP-sensitive K⁺-channel antagonist glibenclamide (Glib) increased [Ca²⁺]_i. 3. In hypoxic condition, 4AP and TEA caused the rise in [Ca²⁺]_i , but Glib had no effect on [Ca²⁺]_i. 4. MTT assay showed that 4AP increased the value of absorbing light degree (A value) in normoxic and hypoxic condition (0.582±0.062,0.873±0.043,respectively, P＜0.01), TEA increased A value only in hypoxic condition, and Glib had no effect on the proliferation of PASMCs. CONCLUSIONS: K_V plays an important role in the regulation of [Ca²⁺]_i and proliferation of PASMCs. K_Ca serves as distinct responsive roles in the regulation of proliferation of PASMCs in hypoxic condition. K_ATP has no effect on [Ca²⁺]_i and proliferation of PASMCs in normoxic and hypoxic conditions.