引进鹅种的性能及杂交利用效果

选择体质健康、体重相近的1日龄四川白鹅、莱茵、朗德鹅和莱×川杂交鹅各40只,在相同的饲养管理条件下,对多项指标进行70d连续测定。试验结果,育雏率依上述顺序穴下同雪分别为72.5%、72.5%、97.5%和65.0%;70日龄体重分别为2985.00g、3280.00g、3295.00g和3550.00g;0～70日龄增重分别为41.43g、45.62g、45.86g和49.51g鸦腿肌率均达到20%以上(26.65%～22.07%)。     

马尾口岸进境废物原料查验区疫情本底调查

2014年8月15 ～ 22日在马尾口岸废物原料查验区采用诱捕、踏查、实验室形态鉴定方法对昆虫、软体动物、杂草等进行调查.结果表明,该次调查共涉及昆虫8目25科35种1 736种次,杂草14科30种,软体动物3科4种143种次.     

马尾口岸进境空集装箱疫情截获分析

对马尾口岸2008~2012年进境空集装箱疫情截获进行统计分析,指出了进境空集装箱植物检疫存在的问题,并提出了相应的检疫监管对策。结果表明,2008~2012年间马尾口岸在进境集装箱空箱中共截获各类有害生物涵盖60科114种,主要来自台湾和香港,并呈季节性变化。进境集装箱空箱携带有害生物疫情复杂,应对进境空箱加强卫生和植物检疫监管,以防止疫情和有毒、有害物质随集装箱携带入境。     

谷物中掺杂油菜籽的转基因成分检测

杂质是谷物及其加工产品中转基因成分污染的一个重要来源,本文对来源于进境谷物中的50份油菜籽样品进行了转基因成分（gox基因、pat基因和bar基因）检测.通过常规定性PCR检测,在31份样品中检出转基因油菜籽,检出率为62%.本研究使用ABI 7700定量PCR仪对油菜籽样品DNA进行了实时荧光PCR定性检测分析,在32份样品中检出含有转基因油菜籽,检出率为64%.在今后要加强进出口谷物中杂质作物的转基因成分检测.     

Inhibitory activities of venom alkaloids of Red Imported Fire Ant against Clavibacter michiganensis subsp. michiganensis in vitro and the application of piperidine alkaloids to manage symptom development of bacterial canker on tomato in the greenhouse

Tomato bacterial canker caused by Clavibacter michiganensis subsp. michiganensis (CMM) is a highly destructive disease that has caused major economic losses in tomato production worldwide. In seeking disease management alternatives, the inhibitory activity of alkaloids extracted from the Red Imported Fire Ant was studied in the laboratory and the greenhouse. Piperidine and piperideine alkaloids each significantly inhibited CMM growth on nutrient agar plates. The inhibitory activity of piperidine alkaloids was stable at 4 ^° C and 22 ^° C for 12 weeks and at 54 ^° C for 4 weeks. The growth of CMM was negatively correlated with the concentration of piperidine alkaloids in nutrient broth. In the greenhouse, piperidine alkaloids also significantly reduced the symptom development on two tomato cultivars, Better Boy and DRK7018F1. This is the first demonstration that piperidine and piperideine alkaloids from the Red Imported Fire Ant are highly inhibitory against a plant-pathogenic bacterium, viz. CMM. Piperidine alkaloids could provide satisfactory management of CMM bacterial canker on tomato seedlings in the greenhouse. Our findings may lead to the development of a new group of bactericides.     

菜青虫危害春甘蓝的产量损失及防治指标研究

采用人工接虫法,探讨了不同密度菜青虫对春甘蓝产量的影响。结果表明,菜青虫百株虫量(x)与甘蓝产量(y)呈显著负相关,相关式为y=165.8904—0.0385x(r=-0.9902)。通过五种曲线模拟及回归分析,得出了虫口密度与甘蓝产量损失率()之间的关系为幂函数,即=0.0208x~(1.0057)(r=0.9961)。提出了豫南地区甘蓝菜青虫防治指标为百株虫量55头。     

进口原羊毛检疫监管中的数字管理

以进口原羊毛检疫监管过程为研究对象,分析提炼检疫监管过程中主要环节的关键数字.通过数字分析,可以在整个进口原羊毛检疫监管链条过程中准确把握产品质量安全的关键问题,提升进口原羊毛检疫监管有效性.     

不同激素对卵母细胞体外成熟培养的影响

本试验对比在成熟液中添加不同来源的促性腺激素,即进口的和国产的促性腺激素(FSH+LH)对山羊卵母细胞体外培养成熟效果的影响。二者对山羊卵母细胞成熟均有促进作用,卵母细胞成熟率都极显著高于对照组(P<0.01),分别为67.3%、68.9%和15.6%,但是它们之间的成熟率差异不显著(P>0.05)。国产激素成本低,使用方便,在卵母细胞体外成熟培养时,完全可以用国产的促性腺激素代替进口的FSH和LH。     

Molecular characterization of low pathogenic avian influenza viruses, isolated from food products imported into Singapore

We have completed the genetic characterization of all eight gene segments for four low pathogenic avian influenza (LPAI) viruses. The objective of this study was to detect the presence of novel signatures that may serve as early warning indicators of the conversion of LPAI viruses to high pathogenic avian influenza (HPAI) viruses. This study included three H5N2 and one H5N3 viruses that were isolated from live poultry imported into Singapore as part of the national avian influenza virus (AIV) surveillance program. Based on the molecular criterion of the World Organisation for Animal Health (OIE), sequence analysis with the translated amino acid (aa) sequence of the hemagglutinin (HA) gene revealed the absence of multibasic aa at the HA cleavage site, identifying all four virus isolates as LPAI. Detailed phylogenetic tree analyses using the HA and neuraminidase (NA) genes clustered these isolates in the Eurasian H5 lineage, but away from the HPAI H5 subtypes. This analysis further revealed that the internal genes clustered to different avian and swine subtypes, suggesting that the four isolates may possibly share their ancestry with these different influenza subtypes. Our results suggest that the four LPAI isolates in this study contained mainly avian signatures, and the phylogenetic tree for the internal genes further suggests the potential for reassortment with other different circulating avian subtypes. This is the first comprehensive report on the genetic characterization of LPAI H5N2/3 viruses isolated in South-East Asia.     

连云港口岸进境木质包装检疫工作分析及建议

通过对连云港口岸2013—2016年进境木质包装检疫情况进行分析,在进境木质包装中共检出各类植物有害生物150种5 025种次,检出植物有害生物占不合格情况批次的80.84%,可见,防止植物有害生物传入是进境木质包装检疫工作的重中之重。同时,指出了进境木质包装检疫工作中存在的实际问题,并就做好进境木质包装检疫工作提出了几点建议。