果蝇生殖包囊固定染色后的荧光特性（摘要）

[目的]探索果蝇卵巢及生殖包囊经过常规固定和染色后的荧光特性,为荧光组织学的发展提供第一手资料。[方法]以黑腹果蝇卵巢为试验材料,用Bouin氏液固定,石蜡切片后分别用HE、苏木精、伊红染色,分别在明场（白光）、绿色、蓝色和紫外激发光下观察、拍照。[结果]3种方法染色后,再经不同激发光激发,生殖细胞和体细胞可发出不同颜色的荧光,细胞中脂类、核酸及蛋白质也可分别发出各自特异性的荧光。[结论]常规染色剂可以分别赋予生殖细胞和体细胞各自不同的荧光特性,也可赋予细胞内不同物质成份各自不同的荧光特性。     

Evaluating landraces of bread wheat <Emphasis Type="Italic">Triticum aestivum</Emphasis> L. for tolerance to aluminium under low pH conditions

Bread wheat (Triticum aestivum L.) landraces held within ex situ collections offer a valuable and largely unexplored genetic resource for wheat improvement programs. To maximise full utilisation of such collections the evaluation of landrace accessions for traits of interest is required. In this study, 250 accessions from 21 countries were screened sequentially for tolerance to aluminium (Al) using haematoxylin staining of root tips and by root regrowth measurement. The staining test indicated tolerance in 35 accessions, with an intermediate response to Al exhibited in a further 21 accessions. Of the 35 accessions classified as tolerant, 33 also exhibited increased root length following exposure to Al. The tolerant genotypes originated from Bulgaria, Croatia, India, Italy, Nepal, Spain, Tunisia, and Turkey. AFLP analysis of the 35 tolerant accessions indicated that these represent diverse genetic backgrounds. These accessions form a valuable set of germplasm for the study of Al tolerance and may be of benefit to breeding programs for expanding the diversity of the gene pool from which tolerant cultivars are developed.     

果蝇生殖包囊固定染色后的荧光特性

[目的]探索果蝇卵巢及生殖包囊经过常规固定和染色后的荧光特性,为荧光组织学的发展提供第一手资料。[方法]以黑腹果蝇卵巢为试材,用Bouin氏液固定,石蜡切片后分别用HE、苏木精、伊红染色,在明场（白光）、绿色、蓝色和紫外激发光下观察、拍照。[结果]3种方法染色后,再经不同激发光激发,生殖细胞和体细胞可发出不同颜色的荧光,细胞中脂类、核酸及蛋白质也可分别发出各自特异性的荧光。[结论]常规染色剂可赋予生殖细胞和体细胞各自不同的荧光特性,也可赋予细胞内不同物质成分各自不同的荧光特性。