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1.
为建立一种针对寨卡病毒的快速诊断方法,本研究根据寨卡病毒的3’端保守基因序列,设计合成1对引物,建立了检测寨卡病毒的荧光定量PCR方法。结果显示:所建立的检测方法的Ct值与标准品在1.41×10^1~1.41×10^10^ copies/μL具有良好的线性关系,相关性为1,斜率为-3.502;灵敏性结果显示,该方法的检测限度为1.41×10^1 copies/μL,是普通PCR的10000倍;特异性结果显示,对CHIKV、DENV和JEV无特异性扩增,特异性强;重复性试验结果显示,组内和组间变异系数均小于1%,重复性好。本研究建立的SYBR Green I real-time PCR检测方法,可用于寨卡病毒感染的快速诊断。 相似文献
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The presentation, diagnostic evaluation, treatment, and 5 years follow-up of a 12-year-old Arabian-Saddlebred cross gelding with neck pain and stiffness, attributable to a fracture of the third cervical vertebrae (C3), is described. Initial cervical spinal radiographs revealed a concave defect in the ventral aspect of the cranial end plate of C3. However, both this finding and ultrasonographic imaging of the area were inconclusive for a fracture. Nuclear scintigraphy revealed that the lesion was metabolically active, prompting computed tomographic imaging that revealed a fracture of C3. Sequential radiographs documented progressive fusion of C2-C3 and no neurological deficits developed over the 5 years after the injury. Cervical vertebral injuries in horses can lead to various clinical signs including ataxia, weakness, and neck stiffness or pain. Diagnosis with cervical radiographs alone can be challenging and, in some cases as the horse in this report, multiple imaging modalities may be required to establish a definitive diagnosis. Horses without neurological signs may recover successfully with conservative medical management, which was performed in this case. 相似文献
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对6种青饲牧草用7种除草剂进行除草试验。证明播后苗前墨西哥类玉米用40%阿特拉津胶悬剂0.90kg(ai)/hm^2;M-81E饲用高粱与串叶松香草适宜用60%丁草胺乳油0.90kg(ai)/hm^2;苏丹草适宜用60%丁草胺乳油0.90kg9ai)/hm^2与25%绿麦隆可湿性粉剂0.94kg(ai)/hm^2,紫苏用12%恶草灵乳油0.27kg(ai)/hm^2与84%chinch乳油1.89 相似文献
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Donald E. Thrall DVM PHD Margaret C. McEntee DVM† Carol Novotney DVM† Marlene L. Hauck DVM† Rodney L. Page DVM MS† 《Veterinary radiology & ultrasound》1993,34(4):295-300
Eighteen dogs with malignant nasal cavity tumors were treated with radiation therapy, including a boost technique. Three 3:0 Gy boost doses were added to a treatment protocol consisting of sixteen 3.0 Gy daily fractions, bringing the total dose to 57 Gy. This boost technique was implemented without an associated increase in overall treatment time by giving the boost doses on a twice-a-day basis. Boost doses were given during the first half of the radiation therapy period. The treatment was completed as planned in 16 of the 18 dogs; two dogs received lower doses (51 and 54 Gy). Median survival was 177 days, poorer than in some other reported studies of nasal tumor irradiation. Acute effects were unacceptable, with 11 of the 18 dogs developing severe mucositis, desquamation, edema, swelling, and pruritus. The extensive nature of the acute reactions compromised assessment of the effect of the increased radiation dose on the tumor. Although there is justification for assessing more aggressive radiation protocols in canine nasal tumor patients, total doses approximating 60 Gy can not be given as described because of the inability of acutely responding normal tissues to compensate. 相似文献
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Modern biotechnology promises a number of new applications in animal breeding and production. Although conventional pig breeding has achieved a high level of efficiency and productivity numerous problems have been encountered with animal health and the loss of meat quality. Selection based on phenotypic performance data of individual animals does not take into account the importance of specific genes and their relevance within a complex regulatory system. In most cases it is therefore difficult to trace back the genetic origins of clinically important disorders. The application of genetic engineering techniques in pig production will facilitate diagnosis, improvement of productivity, and animal health by allowing direct genetic manipulation. Attention must be focussed on the physical and genetic analysis of the procine genome. The isolation and characterisation of genes, DNA-markers, polymorphic DNA-fragments, and their chromosomal assignment will be important prerequisites and tools for the elucidation of genetic disorders. Especially the detection of heterozygous carriers of recessive disorders and their elimination from the breeding stock will increase selection accuracy and decrease the generation intervals. But also the rapid and simple detection of infectious diseases, which is sometimes difficult if not impossible at present, will improve animal health and welfare. Although the production of transgenic animals either by DNA-microinjection into zygotes or the use of embryonal stem cells manipulated in vitro is less straightforward than DNA-based diagnosis it will play an important role in the direct manipulation of the porcine genome and genes. Breeding programmes including the use of transgenic livestock have already been developed. There is no doubt that genetic engineering has reached a degree of practical feasibility, allowing it to play an important role in pig breeding in particular and animal production in general. 相似文献
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Fulmer R Loeb WF Martin DP Gard EA 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1984,13(1):19-25
The intravenous administration of 0.75 gm glucose per kg and the measurement of serum glucose pretest and at 10, 20, 30, 60, 90 and 120 minutes constitute a satisfactory protocol for intravenous glucose tolerance testing of Rhesus (Macaca mulatto) and African Green (Cercopithecus aethiops) monkeys. No significant differences were noted between animals restrained with ketamine hydrochloride and those restrained with sodium pentobarbital, but the African Green males and females and the male Rhesus monkeys yielded significantly different results while being manually restrained. 相似文献
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