糖链－细胞表面蛋白质的信号天线

（河北农业大学生命科学学院,河北保定 071001）     

Lectin histochemistry of the olfactory mucosa of Korean native cattle,Bos taurus coreanae

BackgroundThe olfactory mucosa (OM) is crucial for odorant perception in the main olfactory system. The terminal carbohydrates of glycoconjugates influence chemoreception in the olfactory epithelium (OE).ObjectivesThe histological characteristics and glycoconjugate composition of the OM of Korean native cattle (Hanwoo, Bos taurus coreae) were examined to characterize their morphology and possible functions during postnatal development.MethodsThe OM of neonate and adult Korean native cattle was evaluated using histological, immunohistochemical, and lectin histochemical methods.ResultsHistologically, the OM in both neonates and adults consists of the olfactory epithelium and the lamina propria. Additionally, using periodic acid Schiff and Alcian blue (pH 2.5), the mucus specificity of the Bowman’s gland duct and acini in the lamina propria was determined. Immunohistochemistry demonstrated that mature and immature olfactory sensory neurons of OEs express the olfactory marker protein and growth associated protein-43, respectively. Lectin histochemistry indicated that numerous glycoconjugates, including as N-acetylglucosamine, mannose, galactose, N-acetylgalactosamine, complex type N-glycan, and fucose groups, were expressed at varied levels in the different cell types in the OMs of neonates and adults at varying levels. According to our observations, the cattle possessed a well-developed olfactory system, and the expression patterns of glycoconjugates in neonatal and adult OMs varied considerably.ConclusionsThis is the first study to describe the morphological assessment of the OM of Korean native cattle with a focus on lectin histochemistry. The findings suggest that glycoconjugates may play a role in olfactory chemoreception, and that their labeling properties may be closely related to OM development and maturity.     

糖链－细胞表面蛋白质的信号天线

糖类物质作为生物体的能量来源和结构成分的研究由来已久,对糖链所携带的生物信息功能近年来引起了人们的高度重视。细胞表面糖蛋白是细胞与外界沟通的重要信号分子,糖链的糖基种类和数目、排列顺序和分枝形式、糖链的长短及结合部位的差异,均构成了生物信号,传递着细胞对外界刺激的信息。在此,对细胞表面糖蛋白的生物学效应的研究进展作一综述。