Direct HPLC detection of benzodilactones and quinones in glands of Lysimachia fordiana

The gland cells were successfully collected from the stems of Lysimachia fordiana Oliver, and the homologous pigments of fordianin A, fordianin B, fordianaquinone A and fordianaquinone B were firstly detected in the glands by HPLC. This indicated that the stem was an ideal material for the preparation of the glands, and the gland was a center for the polycyclic pigments accumulation in this species.     

山羊子宫内膜腺上皮细胞的分离培养和鉴定

为了探讨山羊子宫内膜腺上皮细胞的分离、生长条件以及纯度鉴定,通过2 g/L的Ⅰ型胶原酶置于水浴消化4 h,200目的滤网过滤后进行低速离心,收集沉淀物,沉降洗涤3~5次,将细胞移至24孔培养板置37℃细胞培养箱中进行培养。12 h后开始贴壁生长,腺团开始向外部扩散,生长2~3 d,细胞扩散并出现明显的铺路石状,4~5 d腺团基本扩散开来并呈一定走向继续生长。经免疫组织化学方法进行细胞染色,细胞表达角蛋白的阳性率达90%以上。     

Essential Oil Composition of Norwegian Peppermint (Mentha × piperita L.) and Sachalinmint [Mentha sachalinensis (Briq.) Kudô]

Production of varieties of the medicinal plants Mentha