RAPD Analysis of Twelve General Species of Gladiolus hybridus Hort

Gladiolus hybridus Hort., also named Chang or- chid, gladiola, Shi Yang jin and Flat Zhulian, is a perennial bulb plant of Iridaceae Gladiolus and one of the famous four cutting flowers in the world. Gladiolus hybridus Hort. is an exotic species. There is…     

唐菖蒲褐腐病的发生与综合防治

连续3年对唐菖蒲褐腐病进行了系统观测、调查与试验.结果表明,土温20℃时始病,5月下旬至6月下旬(22～24℃)遇高湿阴冷天气形成发病高峰,8月土温高达30℃以上,病害停止发展,10月以后以菌核在土壤中或以菌丝体在病球茎上越冬,成为翌年初侵染源.提出了选留种苗、处理种苗、实行轮作、加强栽培管理、药剂防治的综合防治措施.     

Effect of co-cultivation and crop rotation on corm rot disease of Gladiolus

Field and pot experiments were conducted to evaluate the effect of co-cultivation and crop rotation on the growth and corm rot disease of gladiolus (Gladiolus grandiflorus sect. Blandus) cv. Aarti caused by Fusarium oxysporum f.sp. gladioli (Massey) Snyd. and Hans. In the field experiment, gladiolus was co-cultivated with 10 agricultural/horticultural crops viz. Allium cepa L., Brassica campestris L., Capsicum annuum L., Eruca sativa Mill., Helianthus annuus L., Tagetes erectus L., Zea mays L., Vinca rosea L. and Rosa indica L., in a soil infested with F. oxysporum. All the crops except V. rosea and R. indica reduced disease incidence. The effect of H. annuus and T. erectus was significant and more pronounced than other co-cultivated crops. In general, root and shoot dry biomass, corm fresh weight, number of cormlets and number of flowers per spike decreased as compared to the un-inoculated monoculture gladiolus treatment (negative control) but these parameters enhanced as compared to the F. oxysporum inoculated monoculture gladiolus treatment (positive control). In a pot experiment, all the crops of the field experiment except V. rosea and R. indica were sown in rotation with gladiolus. Pot grown plants of different species were harvested at maturity and the soil was inoculated with F. oxysporum. Gladiolus was cultivated 1 week after inoculation. Disease incidence was significantly suppressed in all the treatments ranging from 29% to 53%. The highest suppression of disease incidence was recorded in T. erectus (53%) followed by B. campestris (49%). The effect of preceding crops on various vegetative parameters was similar in the pot experiment to that of the field experiment. The present study suggests that corm rot disease of gladiolus can be managed by mixed cropping of H. annuus and T. erectus or cultivation of T. erectus and B. campestris in rotation.     

B9和6-BA配合使用对唐菖蒲切花保鲜效果影响的研究

[目的]为提高唐菖蒲切花的保鲜效果提供理论依据及技术指导。[方法]以粉秀唐菖蒲品种为供试材料,通过对唐菖蒲寿命、观赏值、水分平衡值、鲜重变化率、花青素含量、花瓣质膜相对透性等指标的观测,探讨了B9和6-苄基嘌呤(6-BA)的不同组合对唐菖蒲切花保鲜效果的影响。[结果]各处理切花的瓶插寿命均明显长于CK,而添加6-BA和B9的处理其瓶插寿命与母液相比有较明显的延长。6-BA与B9间存在明显的交互作用。6-BA在增加唐菖蒲切花鲜重,改善切花体内的水分状况等方面的作用效果比B9要好。各保鲜剂处理均可抑制植物体内膜脂过氧化物丙二醛的产生,维持细胞膜结构的稳定性。各处理花青素含量随瓶插天数的增加呈先上升后下降的趋势。[结论]综合考虑,以4%蔗糖+300mg/L8-羟基喹啉+150mg/L硼酸+20mg/L6-BA+200mg/LB9的保鲜效果最佳,瓶插寿命达到22d。     

基于表型和SRAP标记的唐菖蒲品种遗传多样性分析

为进一步研究唐菖蒲杂交育种亲本选配和杂种的早期鉴定,以21个唐菖蒲品种为试材,对这些品种的表型性状特征进行聚类分析,并利用SRAP技术对其构建指纹图谱。结果表明:21个品种的表型性状表现出一定的遗传多样性,变异6.78%~147.29%。经过对SRAP-PCR反应条件的优化,从100对引物中筛选出16对引物适用于唐菖蒲PCR反应;通过对21个品种SRAP标记的试验与分析,共在468个位点上扩增出条带,多态性位点411个,平均每个引物组合扩增多态性条带25.7个;基于SRAP标记的UPGMA聚类分析显示,21个品种间Jaccardp’s相似系数0.46~0.75。将2种聚类结果进行比较发现,唐菖蒲品种的分类与表型性状无显著相关性,说明其品种具有复杂的遗传背景。     

ISSR Analysis of M_1 Generation of Gladiolus hybridus Hort Treated by EMS

Gladiolus hybridus Hort is one of famous cutting flowers,being famous of big size,bright color,various shapes and long bloom period.New species should be cultivated in order to meet consumers' needs.Mutagenic breeding is a shortcut to cultivate new species of flowers.In this study,corm bud of G.hybridus Hort was treated with different concentrations of EMS.Then M 1 generation was analyzed by ISSR.Results showed that EMS was a very effective mutagenic agent for the corm bud of G.hybridus Hort.With the increa...     

Cycas necrotic stunt virus isolated from gladiolus plants in Japan

An undescribed spherical virus ca. 30 nm in diameter was isolated from gladiolus (Gladiolus spp.) plants in Japan. The virus had a moderate host range within eight families. Purified virus preparations contained two large RNA components and one coat protein with mobility similar to Cycas necrotic stunt virus (CNSV) from cycas (Cycas revolute). The virus was serologically closely related to CNSV. Its nucleotide sequence of the coat protein gene had 89% common identity with that of CNSV. These results indicated that the virus isolated from gladiolus is a new strain of CNSV. The nucleotide sequence data reported are available in the DDBJ/EMBL/Gen Bank databases under the accession number AB237656.     

唐菖蒲不同品种生理特性及栽培适应性的研究

１９９３～１９９７年研究表明,在贵阳地区,适应性较强的唐菖蒲浅色花品种的休眠球茎、发芽种球、幼苗期球茎及幼苗根的超氧化物歧化酶活性高于适应性较弱的深色花品种,而深色花品种的开花期母球、新球、新根、茎、叶、花药的蛋白质含量以及上部叶片的光饱和点、净光合速率、光呼吸速率、暗呼吸速率高于浅色花品种,花瓣的蛋白质含量低于浅色花品种。由此推测,唐菖蒲不同品种的栽培适应性差异与以上生理生化性状差异有关。     

野生唐菖蒲引种驯化试验

把野生唐菖蒲从远离大陆的海岛上引种到内陆地区，经过2a试验，基本掌握在内陆高山复壮和农地种植技术，引种驯化试验获得初步成功。     

不同浓度的KT和6-BA对唐菖蒲切花的保鲜效应

[目的]研究不同浓度KT及6-BA对唐菖蒲切花的保鲜效应.[方法]以唐菖蒲红色系“青骨红”切花为研究对象,瓶插在以5％蔗糖＋300 mg/L 8-羟基喹啉＋50 mg/L硝酸银为底液,添加不同浓度的激动素（KT）及6-苄基嘌呤（6-BA）的保鲜液中,通过对切花寿命、观赏品质、鲜重变化率的研究,研究其保鲜效应.[结果] KT浓度为30 mg/L时保鲜效果最佳,瓶插寿命比对照延长了2.1d、提高平均日观赏值3.9、切花花枝鲜重峰值推迟2d出现,但对开花率影响不显著;6-BA浓度为40 mg/L时保鲜效果最佳,瓶插寿命比对照延长了2.5d、提高平均日观赏值4.0、提高开花率1.22％、切花花枝鲜重峰值推迟2d出现.[结论]KT、6-BA均能显著提高保鲜剂的保鲜效果.