山竹果皮总黄酮富集工艺研究

研究D101大孔树脂富集山竹果皮总黄酮的工艺。通过静态、动态相结合的方法,确定D101大孔树脂富集的最佳工艺参数。结果表明,D101大孔树脂对总黄酮动态解析附的效果好于静态,动态解析附的最佳条件为:以流速2 m L/min上样30 m L 2 mg/m L的提取物,且用60 m L 70%乙醇以2 m L/min的流速洗脱,此条件下提取物总黄酮含量由原先的57.8 mg/m L增加到174.2 mg/m L。     

Effects of Garcinia cambogia extract on serum sex hormones in overweight subjects

(-) Hydroxycitric acid (HCA), an active ingredient extracted from the Garcinia cambogia fruit rind, has been commonly used as a dietary supplement for weight management. Given the controversy over HCA related testicular toxicity in animal studies, we investigated changes in serum sex hormones levels as an extension of our previous double-blind placebo-controlled trial in human subjects, in which 44 participants received either G. cambogia extract (1667.3 mg/day equivalent to 1000 mg HCA/day) or placebo for 12 weeks. Compared to the placebo group, administration of the extract did not significantly alter the serum testosterone, estrone, and estradiol levels. Similarly, hematology, serum triacylglycerol and serum clinical pathology parameters did not reveal any significant adverse effects. The results of this preliminary investigation indicate that ingestion of G. cambogia extract at dose levels commonly recommended for human use does not affect serum sex hormone levels and blood parameters.     

Cytotoxic alkoxylated xanthones from the resin of Garcinia hanburyi

Three new xanthones, garcinolic acid (1), 10α-ethoxy-9,10-dihydromorellic acid (2), and 10α-ethoxy-9,10-dihydrogambogenic acid (3), along with six known compounds were isolated from the resin of Garcinia hanburyi. These compounds were tested for their cytotoxicities against A549, HCT116, SK-BR-3 and HepG2, and showed high inhibitory effects on the cell lines.     

影响山竹果皮过氧化氢酶活性因素的研究

以山竹果皮中的过氧化氢酶(CAT)为研究对象,对其酶的活性变化及其特性的研究结果表明,山竹果皮中过氧化氢酶的最适温度为30℃,最适pH值为7.2。甲醇和乙醇对山竹果皮中过氧化氢酶活性的影响均表现为低浓度对该酶具有激活作用,高浓度转为抑制作用。金属离子Cu2+对该酶有激活作用,而Na+则对该酶有一定程度的抑制作用。     

大果藤黄乙醇提取物的降血脂与抗氧化性

为充分挖掘大果藤黄的药用功能,利用超声波辅助提取大果藤黄果的活性成分,通过两性霉素B-细胞模型观察大果藤黄乙醇提取物(EG)对细胞内胆固醇生物合成的抑制作用,检测中国仓鼠卵细胞(CHO)的存活率;采用灌胃血脂康和不同剂量的EG建立高脂大鼠模型,测定血清、肝脏和心脏中的胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白以及磷脂含量。结果表明:EG能明显抑制胆固醇生物合成,不同治疗组能明显降低血清、肝脏和心脏中的胆固醇(TC)、甘油三酯(TG)、低密度胆固醇(LDH)的含量和增加高密度胆固醇(HDL)和磷脂的含量;同时,EG也能明显降低肝组织中的MDA含量和提高SOD、CAT和GSH含量。大果藤黄的乙醇提取物具有较好的降血脂和抗氧化效果。     

多花山竹子组培初代培养技术研究

旨在为多花山竹子组培快繁体系建立提供参考。通过观察对比试验法、完全随机化法正交试验设计、运用方差分析、显著性检验、LSD多重比较,探讨多花山竹子组培的外植体最佳消毒时间控制和最适初代培养基类型与激素浓度配方。试验表明：多花山竹子组培的外植体消毒时间为2 min时其污染率最高、死亡率与存活率较低,消毒时间为6 min时其污染率和死亡率居中、存活率最高,消毒时间为10 min时其污染率与存活率最低、死亡率最高;以MS和B5作为多花山竹子组培的初代培养基培养效果较好,若对MS和B5分别添加不同浓度的6-BA、NAA ,对其外植体不定芽的诱导促进作用不同,MS的最佳配比为6-BA 1.6 mg/L+NAA 0.9 mg/L,B5的最佳配比为6-BA 1.6 mg/L+NAA 0.6 mg/L。考虑各因素和激素交互作用,综合认为其外植体消毒时间控制6 min、初代培养基类型与激素浓度配方为MS+6-BA 1.6 mg/L+NAA 0.9 mg/L时培养效果最好。     

热带植物山竹在美容护肤领域应用的研究进展

山竹是一种常见的热带植物,富含黄酮类、多酚、蛋白质和脂类。因其良好的抗氧化活性,以及抗菌,抗炎及抗过敏的功效,日益受到医药学界的重视,其提取物在国外已产业化,并应用于食品及化妆品行业。介绍了目前国内外对山竹的化学成分、抗氧化活性、抗菌活性、抗炎及抗过敏、增强免疫及抗肿瘤等方面的研究进展,旨在加深对山竹的诸多有效化学组分研究,并充分结合临床,尽快用于治疗和预防相关疾病。     

山竹果皮多酚氧化酶酶学特性及抑制效应的研究

多酚氧化酶是酶促反应的关键酶,研究了山竹果皮中多酚氧化酶的活性及不同抑制剂的抑制效果。结果表明:山竹果皮多酚氧化酶的最适pH为5.5,最适温度为30℃,底物浓度与酶活性成正相关;抗坏血酸、亚硫酸氢钠、四硼酸钠、柠檬酸等对该酶表现出不同的抑制作用,抑制作用大小为抗坏血酸>亚硫酸氢钠>四硼酸钠>柠檬酸,抗坏血酸的浓度越高,对酶的抑制效果越明显。     

Antibacterial dihydrobenzopyran and xanthone derivatives from Garcinia cowa stem barks

Two new compounds, garciniacowol (1) and garciniacowone (2) along with 15 known compounds were isolated from the stem barks of Garcinia cowa. Their structures were determined by intensive spectroscopic methods. The structure of 1 was a symmetrical dimeric dihydrobenzopyran derivative, whereas the framework of 2 was a triprenyl caged-xanthone precursor. The antibacterial activities against Escherichia coli TISTR 780, Salmonella typhimurium TISTR 292, Staphylococcus aureus TISTR 1466, and methicillin-resistant S. aureus (MRSA) SK1 of the isolated compounds were also evaluated. Compounds 2 and 9 exhibited good antibacterial activity against MRSA SK1 with the same minimum inhibitory concentration (MIC) value of 2 μg/mL. Moreover, compound 2 also showed good antibacterial activity against S. aureus with an MIC value of 2 μg/mL.     

山竹果皮及果蒂无机元素成分的分析

研究了山竹果皮和蒂中无机元素的组成及含量。将样品消化后定容,利用电感耦合等离子发射光谱仪(ICP)测定。山竹果皮和蒂中分别测出18种和21种无机元素,其中含量较高的元素分别为Ca、Mg、P等常量无机元素。