Effects of the severity and timing of basal leaf removal on the amino acids profiles of Sauvignon Blanc grapes and wines

The effects of the severity and timing of leaf removal(LR) on the amino acids of Sauvignon Blanc grapes and wines were studied during the 2017 growing season. High-performance liquid chromatography(HPLC) was used to analyze the amino acids profiles of grape berries and wines. The basal leaves were removed at three time points(40, 56 and 72 days after flowering, named LR40, LR56 and LR72, respectively) at two severity levels(one at which the first, third, and fifth basal leaves of each shoot were removed(50% level); and another at which the first six basal leaves were removed(100% level)). The results showed that leaf removal had little impact on total soluble solids(°Brix), titratable acidity, pH or berry weight. The LR72-50% treated grapes had higher berry weight, titratable acidity and °Brix than those of the other treatments. The highest concentrations of total amino acids and of total amino acids except proline were detected in LR72-50% treated grapes(2 952.58 and 2 764.36 mg L~(-1), respectively); the lowest were detected in LR72-100% treated grapes(2 172.82 and 2 038.71 mg L~(-1), respectively). LR72-50% treatment significantly promoted the synthesis of aspartic acid, serine, arginine, alanine, aminobutyric acid and proline at both severity levels for grapes, the concentrations of all of these amino acids were increased relative to the control concentrations. The LR72-50%, LR40-100% and LR72-100% treated wines had higher total amino acids concentrations and higher concentrations of some individual amino acids, such as arginine, alanine and serine, than did the control wines. Of all the amino acids studied, glycine, tyrosine, cysteine, methionine and lysine were not significantly influenced by the timing or severity basal defoliation in grapes and wines. The present study reveals the effects of the timing and severity of leaf removal on the amino acids profiles of grapes and wines.     

不同肥力水平下种植密度对浚单29产量性状及产量的影响

[目的]探寻浚单29不同肥力水平地区的适宜种植密度。[方法]研究在高产田与一般田2种肥力水平下浚单29种植密度与产量及其相关产量性状的关系。[结果]2种肥力水平下通过种植密度变化影响穗部性状来影响产量,相同密度,一般田肥力水平下,密度对穗部性状影响大于高产田对穗部性状影响。[结论]在一般田肥力水平下,浚单29最适宜密度为6.75万株/hm2,高产田肥力水平下,浚单29最适宜密度为7.50万株/hm2。     

Effect of miR-29b-mediated TGF-β/Smad signaling pathway on progression of hepatic fibrosis in rats

AIM: To investigate the role of microRNA-29b (miR-29b)-mediated TGF-β/Smad signaling pathway in the activation of hepatic stellate cells (HSC) and its effect on the progression of hepatic fibrosis in rats.METHODS: Hepatic liver fibrosis rat model was established, and its HSC were isolated. Normal rat HSC were also obtained and identified in vitro. RT-qPCR and Western blot were used to detect the alterations of miR-29b, TGF-β/Smad signaling pathway-related proteins and liver fibrosis marker proteins in the acquired cells. Finally, the direct targeting binding of miR-29b to TGF-β1 was identified by dual-luciferase reporter assay system.RESULTS: With the activation of HSC, the expression of miR-29b gradually decreased (P<0.01), while the expression of collagen type I and α-smooth muscle actin gradually increased (P<0.01). At the same time, the expression of Smad2/3/4 was significantly increased, and the expression of Smad7 was significantly decreased (P<0.01). Dual-luciferase reporter assay showed that miR-29b bound directly to "UCUCUCCGU" in the 3'UTR of TGF-β1, indicating that TGF-β1 was a downstream target gene of miR-29b.CONCLUSION: miR-29b may be involved in the inhibition of HSC activation and migration, thereby inhibiting the process of liver fibrosis. The biological function of miR-29b may be through the direct targeting of TGF-β1, thus regulating and inhibiting the TGF-β/Smad signaling pathway.     

籼稻Wx基因与稻米食用品质的相关性分析

以14个水稻品种及亲本为材料,通过检测各材料的Wx基因型,测定其直链淀粉含量(AC)、胶稠度(GC)和糊化温度(GT),对参试品种的Wx基因与AC、GC和GT的相关性进行了分析.结果表明,基因型为GG的品种具有较高的AC和较低的GC,而TT型品种具有中等的AC和较高的GC;AC与GC、GT呈极显著负相关,说明Wx除了控制稻米的AC之外,Wx与之紧密连锁的基因位点还可能影响GC的高低程度和GT的大小.对属于同一个恢复系的杂交组合的不育系和品种之间作偏相关性分析表明,优良品种的AC与不育系呈显著负相关,而品种的GC和GT与不育系之间的相关性却没有那么明显.     

Heterologous expression of the ThIPK2 gene enhances drought resistance of common wheat

Th IPK2 is an inositol polyphosphate kinase gene cloned from Thellungiella halophila that participates in diverse cellular processes. Drought is a major limiting factor in wheat(Triticum aestivum L.) production. The present study investigated whether the application of the Th IPK2 gene could increase the drought resistance of transgenic wheat. The codon-optimized Th IPK2 gene was transferred into common wheat through Agrobacterium-mediated transformation driven by either a constitutive maize ubiquitin promoter or a stress-inducible rd29 A promoter from Arabidopsis. Molecular characterization confirmed the presence of the foreign gene in the transformed plants. The transgenic expression of Th IPK2 in wheat led to significantly improve drought tolerance compared to that observed in control plants. Compared to the wild type(WT) plants, the transgenic plants showed higher seed germination rates, better developed root systems, a higher relative water content(RWC) and total soluble sugar content, and less cell membrane damage under drought stress conditions. The expression profiles showed different expression patterns with the use of different promoters. The codon-optimized Th IPK2 gene is a candidate gene to enhance wheat drought stress tolerance by genetic engineering.     

亚洲璃眼蜱唾液腺-新基因（GP29）的原核表达及产物鉴定

将吸血诱导的亚洲璃眼蜱唾液腺差异表达基因GP29的开放性阅读框插入pGEX-4T-1，转化BL21，表达出一相对质量为53ku的蛋白，其大小与预计结果一致。蛋白质的肽质量图谱和“1381．7511”肽段序列两方面的结果证明，SDS-PAGE胶板上相对质量为53ku的蛋白就是由目的基因表达的GST融合蛋白。该蛋白与Swiss PROT库中的任何蛋白均有较大差别，可能是一个新功能分子。     

狂犬病病毒感染对宿主miR-29a和miR-124表达的影响及其靶基因分析

依据本实验室前期狂犬病病毒(RABV)感染小鼠原代神经元microRNA(miRNA)表达谱数据,选取表达显著上调的miR-29a和下调miR-124为研究对象,采用实时荧光定量PCR技术检测目的 miRNA在RABV感染前后的小鼠原代神经元和小鼠海马组织内的表达变化。运用TargetScan数据库预测目的 miRNA潜在的靶基因,并对靶基因进行了GO(Gene Ontology)注释描述、GO注释富集分析和信号通路富集分析,富集结果呈现出数个与神经功能相关的GO注释和信号通路。本研究结果提示miR-29a和miR-124可能参与了RABV感染所致的宿主神经功能异常。     

The borders within: Mobility and enclosure in the Riau Islands1

Abstract: The border studies literature makes a strong case against claims for unfettered transnationalism and ‘borderlessness’ in our ‘globalising world’. However, its focus on movement across borders means that it fails to address bordering practices that occur within the nation‐state as a result of transnational activity. In this paper, we extend Cunningham and Heyman’s concepts ‘enclosure’ and ‘mobility’ to confront the different layers of bordering (both physical and non‐physical) that have occurred in Indonesia’s Riau Islands since they became part of the Indonesia–Malaysia–Singapore Growth Triangle.     

杂交水稻寻杂29种子蛋白质组分的研究

 本研究用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)分析寻杂29单粒种子中清蛋白、球蛋白、醇溶蛋白和谷蛋白的亚基组成.SDS-PAGE表明,没有巯基乙醇(ME)时,谷蛋白以二硫键连接亚基呈高分子量形式存在.寻杂29和常规品种云-1比较,谷蛋白在溶解性,结构方面存在差异.寻杂29与其三系亲本,以及云-1比较,它们的种子盐溶蛋白在多肽组成和含量上存在明显差异.水稻种子盐溶蛋白的差异可考虑作为种子纯度判断的指标.     

益生芽孢杆菌B.licheniformis29质粒Pbl29性质的研究

质粒pBL29为闭合环状质粒，酶切分析证明质粒pBL29有大量的单酶切位点，如EcoR I、Sma I、Hind Ⅲ、Bgt Ⅱ、Pst I、Xba I、BamH I等，并根据限制性酶切各片段的分子量作出了质粒pBL29的内切酶图谱。对质粒pBL29进行测序和分析，证明了其大小为3711bp，具有大量的单酶切位点、编码卡那霉素抗性基因以及富含A／T序列的复制起点。