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Gas and short‐chain fatty acid production from feeds commonly fed to red deer (Cervus elaphus L.) and incubated with rumen inoculum from red deer and sheep
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Andrej Lavrenčič Darko Veternik 《Journal of animal physiology and animal nutrition》2018,102(5):1146-1153
Efficient red deer supplementary feeding depends on estimations of the nutritive value of offered feeds, frequently estimated with the use of equations derived from domestic ruminants. The aim of this study was to compare the 24‐hour in vitro true dry matter degradability (ivTD24), in vitro gas production (GP) kinetic parameters, GP in 24 hr of incubation (GAS24) and short‐chain fatty acid (SCFA) and microbial biomass (MBS) produced after 24‐hour incubation of feeds in inoculum prepared from sheep and red deer rumen fluid. Eleven feeds, frequently consumed by red deer in Slovenia, which occur either naturally (two fresh grasses, chestnut fruits and common and sessile oak acorns) or are fed as winter supplemental feeds (two grass hays, two grass silages, apple pomace, fresh sugar beetroot), were investigated. The in vitro GP kinetic parameters, GAS24 and ivTD24, did not differ between animal species. Amounts of SCFAs were greater (p < 0.05) when feeds were incubated in sheep inoculum, while molar proportions of acetic and propionic acids did not differ. Molar proportions of butyric acid produced during incubation of high fibre feeds did not differ between animal species, but were higher (p < 0.05) when feeds high in starch or sugar were incubated in red deer inoculum. Greater production of SCFA by sheep rumen microbes suggests better coverage of host animal with energy precursors, while greater production of MBS by red deer rumen microbes suggests better coverage of host animal with protein. Results also suggest that rumens of sheep and red deer are inhabited by different microbial communities, which did not affect the extent of in vitro GP and degradation of feeds used in the present experiment. However, the possibility exists that the divergent nutrient use could be a consequence of different priming by different feeds of the donor animal diets. 相似文献
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光敏色素是一类红光/远红光受体,对植物光形态建成、外部形态和生理功能等方面起着重要的调节作用。为明晰光敏色素A在调控高粱光形态建成发育过程中的作用,本研究比较分析光温敏感程度不同的高粱品种Btx623、Rio、Tx430和LH645的PhyA基因序列和PHYA蛋白的保守结构域,利用qRT-PCR明确长短日照条件处理下PhyA基因的表达模式。结果表明,Btx623、Rio和Tx430之间PhyA基因序列存在非同义突变,LH645在PhyA基因的第7个外显子上插入了5 bp碱基序列;高粱PhyA蛋白中含有1个PAS-2结构域、1个GAF结构域、1个Phytochrome结构域、2个PAS结构域、1个组氨酸激酶A结构域和1个类似组氨酸激酶的ATP激酶结构;遗传进化树分析表明,单子叶和双子叶植物PhyA蛋白明显聚为2大类,高粱PhyA、谷子PhyA和玉米PhyA1、PhyA2聚为一个亚类,相互间亲缘关系较近,与水稻PhyA同源性相对较远;在短日照(SD)条件下各时期PhyA基因转录水平变化显著,Rio PhyA基因转录水平较Btx623和Tx430变化明显,LH645 PhyA基因转录变化不显著,在长日照(LD)条件下Rio PhyA基因转录水平低于Btx623和Tx430,LH645 PhyA基因转录水平变化不显著。以上结果表明,PhyA受光周期诱导,推测其在高粱光形态建成中起重要调控作用。本研究为进一步解析PhyA基因功能及其在调控高粱光形态建成发育过程中的作用提供理论基础。 相似文献
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《畜牧兽医杂志》2012,39(3)
进行了4个钢筋混凝土(RC)超短柱、4个型钢混凝土(SRC)超短柱和6个RC分体柱的滞回性能试验研究.8个超短柱的剪跨比为1;6个分体柱的剪跨比为2.4.试验结果表明,RC超短柱的变形能力不满足现行抗震规范要求.不设剪力连接件的SRC超短柱的破坏模式为黏结破坏,与RC超短柱相比,其抗剪承载力和变形能力并无明显提高;设置剪力连接件的SRC超短柱的破坏模式为斜压破坏,其抗剪承载力和变形能力显著提高,但构件的变形能力仍然难以满足现行抗震规范要求.试验结果还表明,在RC超短柱中开缝,形成剪跨比大于2的RC分体柱,可有效提高柱子的延性和变形能力.对于RC分体柱,随轴压比的提高,构件的抗弯承载力提高,但延性和变形能力下降;当设计轴压比大于0.7时,RC分体柱的变形能力不满足现行抗震规范要求.根据试验结果,提出了SRC超短柱的剪力连接件设计方法和RC分体柱的设计建议. 相似文献
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Apache Struts is one of the most popular open source framework for Web applications and developments using MVC Model2 architectural pattern. Using the Information Technology to improve the efficiency of education service is emergent. The aim of this paper is to demonstrate the design and realization of a platform system software based on Apache Struts framework. By briefly analyzing the extra requirement in higher education service, an architecture based on MVC design pattern is presented. Some core technologies used in the platform system software, such as Struts Tiles and Struts Menu etc, are proposed in details by the end. Because of its struts-based fundamental architecture and running mechanism, the platform system software made a certain performance advantage and largely reduced the developing period. 相似文献