Induction of apoptosis with Salvia miltiorrhiza monomer IH764-3 via downregulating focal adhesion kinase in H2O2-stimulated hepatic stellate cells

AIM: To investigate the effect of IH764-3 on the apoptosis of H₂O₂-stimulated hepatic stellate cells(HSCs) and the alteration of focal adhesion kinase (FAK). METHODS: The proliferation of HSCs was examined by direct cell count and the apoptosis was determined by Annexin-V/PI labeling, while the morphological change was observed by light microscopy and transmission electron microscopy. In addition, FAK mRNA was detected by RT-PCR. RESULTS: H₂O₂ promoted the proliferation of HSCs. IH764-3 induced the apoptosis of HSCs in a dose-dependent manner. The HSC apoptotic rates of different groups were 6. 35%,9. 28%,15. 10%,19. 69%,respectively, after treated with different concentrations of IH764-3 for 48 h while H₂O₂ group showed 2. 30%. In 30 mg/L group, the apoptosis rates were 6. 73%、10. 34%、15. 10% for the indicated time periods(12 h, 24 h, 48 h). In the presence of IH764-3, FAK mRNA decreased. The FAK mRNA reduction began at 2 h after adding IH764-3. CONCLUSION: IH764-3 induced the apoptosis of HSCs. Down-regulating the expression of FAK mRNA may be one of its mechanisms.     

钢管结构法兰连接节点抗弯承载性能的试验研究

研究了钢管结构法兰连接节点的抗弯承载性能.通过试验方法，采用螺栓应变测量装置，对含4种基本形式的法兰连接节点的试件进行四点弯加载试验，考察了在节点受弯过程中螺栓和法兰板的受力特性.试验得到了其屈服荷载与极限荷载，并且与相应的有限元分析结果吻合良好.试验结果表明，法兰连接节点的受拉区存在明显的撬力作用，刚性法兰连接节点的撬力作用小于柔性法兰连接节点.法兰连接节点的安全储备约为1.2.     

阶梯式连续梁的有限三弯矩方程及刚度分析

应用迭代法对阶梯式简支梁挠曲线的有限差分方程组进行分析、求解，建立有限转角及挠度方程，推导了阶梯式连续梁的有限挠度方程及有限三弯矩方程，并据该方程进行了弯曲刚度分析，得到足够精确的结果，为工程计算提供了一个很好的方法。     

脑络通胶囊对大鼠局灶性脑缺血模型的影响

目的观察脑络通胶囊对大鼠大脑中动脉缺血模型的影响。方法通过线栓法建立大鼠大脑中动脉(MCA)局灶性脑缺血模型,动物经给药3次,观察脑络通胶囊对脑缺血大鼠的行为状态、脑缺血面积、脑组织形态学的作用。结果脑络通胶囊1.72、0.86、0.43g/kg剂量均能明显改善大鼠脑缺血后的行为状态,缩小缺血范围,降低缺血程度,使病变的脑组织得到明显地改善。     

打造"焦点时刻"教育品牌探索提高大学生思想道德修养课教学实效性的有效途径

大学生思想政治理论课的实效性一直是人们普遍关注的焦点。从创新大学生思想道德修养课课外教学形式入手,以“三贴近”为出发点,以直接面对大学生现实思想问题和困惑为切入点;以“主体—主体”间的双向交流互动为手段,通过举办“焦点时刻”教育栏目,正确引导学生关注热点问题,取得了明显的实效。     

基于核心主导人物构建团队的拓扑形态分析

以核心主导人物构建团队的方式,运用点集拓扑学中凝聚点的概念,将核心主导人物看作团队的凝聚点,得到核心主导人物集合是团队拓扑空间的闭集,并由此对由3个成员的团队进行分析,得出了同胚意义下的所有拓扑形态。     

Influence of high-mobility group box 1 on proliferation of neural stem cell in pre-infarction cortex of focal cerebral ischemia/reperfusion model rats

AIM：To investigate the influence of high-mobility group box 1 (HMGB1) on the proliferation of neural stem cells in peri-infarction cortex of focal cerebral ischemia/reperfusion model rats. METHODS：Male SD rats (n=48) were randomly divided into sham group, ischemia/reperfusion (I/R) group, RNA interference group and negative interference group. The rat middle cerebral artery was blocked to establish focal cerebral I/R model (ischemia for 1 h and reperfusion for 7 d). Lentivirus vector of HMGB1 shRNA was used to suppress the HMGB1 protein expression in the rat brain. The effect of RNA interference was evaluated by the methods of double-immunofluorescence labeling of HMGB1/GFAP and Western blotting. The proliferation of neural stem cells in the peri-infarction cortex was assessed by double labeling of BrdU/nestin. RESULTS：The protein expression of HMGB1 in I/R group was much higher than those in sham group (P<0.05). RNA interference effectively inhibited the HMGB1 expression (P<0.05). Double labeled BrdU/nestin positive cells in I/R group were more than that in sham group (P<0.05). The double labeled BrdU/nestin positive cells were significantly decreased in RNA interference group (P<0.05). CONCLUSION：Focal cerebral ischemia/reperfusion injury promotes the proliferation of neural stem cells in peri-infarction cortex by increasing HMGB1 protein level.     

轮胎试验台六分力解算、标定与优化分析

轮胎试验台的力与力矩测试精度直接影响到轮胎数据的准确性,应用多体动力学理论对所研发的平板式六自由度低速轮胎试验台进行研究,分析了该试验台姿态控制的机理,推导出试验台轮胎六分力解算公式,并用abaqus有限元软件验证其准确性。然后对设计加工出的试验台进行标定,结果表明:3个方向的力吻合较好,但是力矩并不特别理想。经合理分析假设,试验台6个电缸铰支的硬点实际安装中存在空间的偏差,对其进行辨识优化分析,结果表明:优化后三方向的力及力矩与标定值吻合的精度符合要求,力的精度高于0.75%,力矩精度可达1%左右,证明了铰支安装偏差的假设是合理的,也体现出该轮胎试验台在测试精度上的优势。     

计算刚架的矩阵迭代法

本文在迭代法基础上推导出矩阵迭代法的计算公式。用以编制程序,可节省容量,尤其适用于袖珍计算机。     

基于不变矩和神经网络的泵机组轴心轨迹自动识别

基于泵机组故障信号处理的需要,介绍了不变矩原理,同时对神经网络建模,包括其样本获取进行了详细讨论;由于泵机组的多种故障与表征其运行状态的轴心轨迹形状有关,根据不变矩的平移、伸缩和旋转不变性特征,对实时检测的轴心摆度信号进行不变矩处理,利用BP型神经网络对其进行模式识别,进而判断出轴心轨迹的形状．为了弥补泵机组用于神经网络训练样本的不足,采用数值模拟与现场测试相结合的方法,将获取的所有样本进行求不变矩处理,并连同样本对应的实际形状作为神经网络的训练样本．网络训练完成后,将其输出结果与轴心轨迹图形进行比较验证．以山西大禹渡泵站水泵机组故障检测及诊断为例,在样本中选取其中的10组数据,比较的结果表明神经网络自动识别的结果准确．该方法为泵机组轴心轨迹自动识别和实现泵机组故障诊断智能化提供了依据．