全文获取类型
收费全文 | 2449篇 |
免费 | 86篇 |
国内免费 | 117篇 |
专业分类
林业 | 349篇 |
农学 | 197篇 |
基础科学 | 51篇 |
121篇 | |
综合类 | 1196篇 |
农作物 | 120篇 |
水产渔业 | 50篇 |
畜牧兽医 | 234篇 |
园艺 | 253篇 |
植物保护 | 81篇 |
出版年
2024年 | 5篇 |
2023年 | 25篇 |
2022年 | 34篇 |
2021年 | 50篇 |
2020年 | 50篇 |
2019年 | 61篇 |
2018年 | 28篇 |
2017年 | 60篇 |
2016年 | 97篇 |
2015年 | 89篇 |
2014年 | 136篇 |
2013年 | 122篇 |
2012年 | 185篇 |
2011年 | 214篇 |
2010年 | 170篇 |
2009年 | 148篇 |
2008年 | 156篇 |
2007年 | 169篇 |
2006年 | 142篇 |
2005年 | 130篇 |
2004年 | 83篇 |
2003年 | 84篇 |
2002年 | 51篇 |
2001年 | 58篇 |
2000年 | 43篇 |
1999年 | 42篇 |
1998年 | 33篇 |
1997年 | 21篇 |
1996年 | 26篇 |
1995年 | 28篇 |
1994年 | 21篇 |
1993年 | 11篇 |
1992年 | 16篇 |
1991年 | 17篇 |
1990年 | 12篇 |
1989年 | 9篇 |
1988年 | 4篇 |
1987年 | 6篇 |
1986年 | 4篇 |
1985年 | 4篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1979年 | 1篇 |
1975年 | 1篇 |
排序方式: 共有2652条查询结果,搜索用时 31 毫秒
1.
科技情报作为一种重要的信息媒介。作者着重地阐述了其具备的宏观俯瞰,微观入里的导引功能。就其科技情报意义与林业科研及生产领域上的信息交流而言提出了自己的见解。 相似文献
2.
当前,面对加入WTO和全面建设小康社会的新形势、新任务,有必要大力发展农村中介组织以发展农村经济、增加农民收入。本文拟通过近年来福建农村中介组织发展的状况、存在问题的分析研究,提出加快发展壮大农村中介组织的思路与对策。 相似文献
3.
4.
R. Thomassen 《Reproduction in domestic animals》1993,28(4):289-293
Contents: An insemination trial using frozen semen is described. The freezing procedure was slightly modified from the Hannover method. The insemination dose consisted of 7 medium straws containing approx 1 109 spermatozoa. A total of 28 mares of the Norwegian Trotter breed were inseminated during the 1991 season. During oestrus the mares were examined at 12 hour intervals, and the insemination was carried out after detection of ovulation. The pregnancy rate was 43% after the first insemination, increased to 68% after second and further to 75% after the third and last insemination. The foaling rate was 61.5%. 相似文献
5.
AIM:To investigate the effect of insulin on ox-LDL transferring the THP-1 cells to foam cells and influencing the LPL mRNA expression in THP-1 cells.METHODS:THP-1 cells were incubated with 50 mg/Lox-LDL and insulin at concentrations of 10 mU/L, 100 mU/L, 1 000 mU/L and 10 000 mU/L, respectively. The expression of LPL mRNA in cells was detected by RT-PCR. Lipoprotein lipase of THP-1 cells was presented by no-specific lipase staining. THP-1 cells were stained with oil red O. Accumulation of total cholesterol (TC) in THP-1 cells was determined with oxidase assay.RESULTS:In 100 mU/L、1 000 mU/L、10 000 mU/L insulin groups, LPL mRNA expression increased 2 times, the average cell perilength was longer, the percentage of positive oil red O staining cells was significant higher, the content of cholesterol in THP-1 cells was higher than in ox-LDL control (P<0.05).CONCLUSION:Insulin accelerates transferring of THP-1 cells to foam cell with exposed to ox-LDL because LPL mRNA expression increased in the cells. 相似文献
6.
7.
AIM:To explore the effects of oxidized low density lipoprotein (OxLDL) and one of its component— lysophosphatidylcholine (LPC) on cholesterol efflux from mouse macrophage foam cells.METHODS:(1) Cholesterol efflux induced by apoAI from mouse peritoneal macrophage foam cells loaded with OxLDL or acylated LDL(AcLDL) was measured. (2) Cholesterol efflux induced by LPC and apoAI from macrophage foam cells separated from normal or apoE gene deficient (E0) mouse loaded with AcLDL were measured.RESULTS:(1) When the macrophage foam cells were incubated with apoAI, cholesterol efflux from AcLDL-induced macrophage foam cells increased significantly compared to that of OxLDL-induced macrophage foam cells. (2) LPC promoted cholesterol efflux from macrophage foam cells in relation to both dosage and time. When LPC was incubated with E0 mouse macrophage foam cells, the released cholesterol mass was significantly lower than that of normal mouse macrophage foam cells. It was also found that cholesterol efflux induced by apoAI normally occurred in E0 mouse macrophage foam cells.CONCLUSIONS:(1) OxLDL accumulated cholesterol in macrophages and impair cholesterol efflux. (2) LPC induced cholesterol efflux from macrophage foam cells, which may occur via apoE pathway. 相似文献
8.
灵芝胞外多糖高产菌株筛选及其深层发酵培养基的优化 总被引:12,自引:2,他引:12
运用反馈抑制理论构建了耐灵芝胞外多糖 (EPS)反馈抑制的筛选模型。添加在筛选培养基中的其指示因子同源胞外多糖浓度为 2 .34g/L。将实验室保藏的 37株灵芝菌株输入该模型 ,即可检出耐灵芝胞外多糖反馈抑制作用最强、产胞外多糖能力最强的菌株GL0 2 9。然后在基础培养基的基础上用正交试验方法优化GL0 2 9深层发酵培养基。结果表明 ,组合碳源和组合氮源培养基最适合该菌株深层发酵生产灵芝胞外多糖。深层发酵培养基配方为 :蔗糖 10 g/L ,玉米粉15 g/L ,蛋白胨 2 g/L ,酵母膏 1g/L ,KCl 0 .5g/L ,KH2 PO4 ·7H2 O 0 .5 g/L ,pH自然。于 30℃、12 0r/min摇瓶培养 9d ,该菌株的胞外多糖产量高达 3.0 7g/L。 相似文献
9.
采用均匀设计法优化红汁乳菇液体深层发酵培养基。结果表明,最佳培养基配方是:蔗糖34g]L,废糖蜜13mL/L,麸皮36g/L,玉米粗粉20g/L,KH2PO43g/L,菌丝体最大生物量达18.087g/L。 相似文献
10.
鸡腿蘑菌丝体的同工酶研究 总被引:7,自引:1,他引:7
用8种不同培养基配方,培养鸡腿蘑菌丝体,其同工酶分析结果表明,鸡腿蘑菌丝体酯酶(EST)同工酶谱差异明显,过氧化物酶(POD)同工酶谱差异明显。 相似文献