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排序方式: 共有86条查询结果,搜索用时 15 毫秒
1.
鲟鱼细菌性败血症的诊断初报 总被引:1,自引:0,他引:1
河南省信阳市和厚特种水产养殖场 2 0 0 0年 9月发生俄罗斯鲟、杂交鲟大量发病 ,经临床观察、病理检查及病原体分离培养等 ,确定鲟鱼细菌性败血症是由嗜水气单胞菌所引发。分离菌株对多种抗菌药敏感 ;毒力试验表明 ,分离菌株对小白鼠的MLD为 2 0亿细菌 ,用抗菌药物拌饵投喂 ,结合消毒换水 ,有效地控制了该病 相似文献
2.
鱼类细菌性败血症发病鱼池细菌数量及致病性气单胞菌的初步研究 总被引:2,自引:0,他引:2
本文对细菌性败血症发病鱼池细菌数量及气单胞菌的数量、溶血性及致病力进行了研究。结果表明:水温在28.5℃以下时,细菌数量与水温基本成正比,发病池与不发病池的细菌数量无显著性差异;水体中广泛存在气单胞菌,其占水体总菌量比例随水温的上升而升高;发病池及未发病池中均存在能产生hec毒素和不产生hec毒素的嗜水气单胞菌株,两者数量上无显著差异;水体中存在血清型为TPS—30及PBJS—76的嗜水气单胞菌,且大多数对鱼体有毒力,以5×10~7CFU/尾腹腔注射,可使试验鱼大部分或全部发病死亡。 相似文献
3.
J'mai M. Gayle Noah D. Cohen M. Keith Chaffin 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1998,12(3):140-146
In order to identify variables obtained at admission that could be used to predict survival in septicemic foals, medical records of 65 foals diagnosed with septicemia were reviewed. Initially, variables were analyzed independently (univariate analysis) for association with survival. Of the physical examination and historical data examined using univariate analysis, the ability to stand at admission, respiratory rate ≥ 60 breaths per minute (bpm), and normal-appearing mucous membranes were significantly associated with survival. Foals with history of induced parturition were significantly less likely to survive. The following hematologic and serum biochemical variables determined at admission were significantly associated with survival: white blood cell count a 6,000 cells/μL, neutrophil count <4,000 cells/μL, serum albumin concentration <2.2 g/dL, serum glucose concentration <120 mg/ dL, blood pH ≥ 7.35, and positive base excess. The administration of plasma at admission was significantly associated with survival. Stepwise multiple logistic regression analysis was performed to evaluate the association between survival and variables identified as significantly associated with survival in bivariate analysis. The final multivariate model selected included the variables standing, duration of clinical signs (24-hour intervals) prior to admission, respiratory rate a 60 bpm, neutropenia (> 4,000 cells/μL), and neonatal age category. The probability of survival was significantly increased for foals that were standing, had a respiratory rate a 60 bpm, and that had a neutrophil count <4,000 cells/μL at admission. Probability of survival was significantly decreased for foals that had a longer duration of clinical signs prior to admission. For each 24 hours of duration, the estimated risk of death was increased by 5.8-fold. 相似文献
4.
Yuko TAJIMA Kaori MAEDA Tadasu K. YAMADA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):45-51
One hundred and twenty
stranding events of Stejneger’s beaked whales were reported in Japan between 1999 and
2011. The purpose of this study is to introduce pathological data and to discuss probable
causes of death for 44 Stejneger’s beaked whales among them. The significant pathological
findings were the pulmonary edema, parasitic granulomatous nephritis, emaciation,
amyloidosis, suppurative bronchopneumonia and so on. The probable causes of death were
categorized as noninfectious in 43 of the cases, which included drowning, starvation and
secondary amyloidosis. One individual was diagnosed with septicemia, which was the only
example of an infectious disease. Because we could not always perform advanced analyses,
such as microbiology tests, biotoxin examinations or contaminant analyses, the finality of
our findings may be impaired. However, the present study has broad implications on the
causes of death of Stejneger’s beaked whales of the seas around Japan, which are valuable
for the future studies and for the detection of emerging diseases. 相似文献
5.
β-1,3-葡聚糖及其羧甲基衍生物对海湾扇贝免疫功能的影响 总被引:3,自引:0,他引:3
2003年3月和2004年4月,新疆某养殖场和西安灞桥区某养殖场的白斑狗鱼(Esox lucius Linnaeus)相继爆发“败血症”。从具有明显症状的濒死病鱼体表病灶和内脏组织分离得到12株优势菌株,经人工感染试验表明:其中10株分离菌为致病菌。对分离菌株进行形态、培养特征、生理生化试验鉴定,认为该病原菌为杀鲑气单胞菌史氏亚种(Aeromonas salmonida subsp.smith)。血清学试验表明各分离菌株具有相同的保护性抗原。组织病理学研究表明:患病狗鱼病灶肌纤维明显肿胀断裂,肌纤维束之间出现间隙;肝胰组织严重坏死,细胞核溶解消失,细胞浆崩解;肾小管绝大部分均已坏死,细胞破裂,胞浆呈红色团块状流失于管腔。脾脏细胞大量坏死,髓质内含铁血黄素沉积。代表菌株EL0304-1药敏试验结果为:对供试24种抗菌药物中的磺胺二甲嘧啶钠、磺胺二甲嚯唑钠等不敏感;庆大霉素、卡那霉素等中度敏感;对氧氟沙星、培氟沙星、新霉素等高度敏感。 相似文献
6.
7.
本文从患败血病的中国对虾体内分离到多株细菌,其中三株经人工感染证实为病原菌,又经40多项形态、生理、生化特性测试,鉴定为雷氏普罗威登斯菌(Providenciaretgeri),其主要特性为杆状,革兰氏阴性,周生鞭毛,运动,无自然游动现象,兼性厌氧,苯丙氨酸脱氨酶、柠檬酸盐利用、吲哚、甲基红反应均阳性,发酵甘露醇、肌醇、甘露糖产酸。赖氨酸、鸟氨酸脱羧酶、精氨酸双水解酶、VP反应均阴性。生长最适温度、NaCl、pH范围分别为25℃—30℃、05%—15%、70—90。对羧苄青霉素、先锋霉素5号、氯霉素、SMZ+TMP、氟哌酸、链霉素等极为敏感。雷氏普罗威登斯菌引起对虾败血病在国内外尚属首次报道。 相似文献
8.
将温和气单胞菌(Aeromonas sobria)复壮,在28℃条件下加福尔马林灭活48h,制备成灭活苗。用该疫苗免疫团头鲂,取免疫后第10d,20d,30d的血清做凝集抗体试验,最高凝集抗体效价达1:32~128,对照组为1:4~8;免疫鱼的抗感染保护力达70%。 相似文献
9.
鲤鱼细菌性败血症的病理学研究 总被引:16,自引:0,他引:16
汪开毓 《四川农业大学学报》2004,22(3):257-262
报道了由嗜水气单胞菌嗜水亚种 (Aeromonashydrophilasubsp .hydrophila)感染鲤鱼引起细菌性败血症的病理学变化 ,通过对自然感染和人工感染病例的病理学观察 ,病理变化主要表现为鱼体多处出血 ,特别是体表的出血最为明显 ,上下颌、眼睛、鳃盖、鳍基充血发红 ,皮肤有瘀斑瘀点 ,并发生糜烂、溃疡 ;腹水 ,肛门红肿外突 ;心、肝、肾、肠等内脏器官充血、出血、肿大、变性、坏死、发炎以及红细胞发生溶血等 ,表现出细菌性败血症的病理特征 ,并在病理变化观察的基础上探讨了该病发生的机理。 相似文献
10.
Peek SF Borah S Semrad S McGuirk S Slack JA Patton E Coombs D Lien L Darien BJ 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2004,33(1):29-31
Background: Accurate determination of plasma endotoxin concentration is critical for ex vivo and in vitro cellular and molecular studies of endotoxemia in horses. However, reports are conflicting with respect to anticoagulant, handling, and sample preparation.
Objective: The purpose of this study was to determine the effect of blood sample fraction and handling time on measurement of endotoxin concentration in horses.
Methods: Whole blood, anticoagulated with 3.8% (0.12 M) sodium citrate (9:1), was collected from 5 healthy horses. Whole blood (WB), platelet-rich plasma (PRP), and platelet-poor plasma (PPP) were spiked with endotoxin (2 EU/mL). Endotoxin-spiked WB samples were centrifuged immediately to generate PRP for measurement. Endotoxin concentration was subsequently measured by Limulus amebocyte assay at 0, 15, 30, 45, and 60 minutes. Assays were performed in triplicate and results were analyzed using Student's t -test, with significance set at P < .05.
Results: Mean endotoxin concentrations in 2 EU/mL-spiked WB were significantly different from those in PPP at all time points tested. Recovery of endotoxin in PRP generated from WB was significantly diminished after just 15 minutes.
Conclusion: PRP generated from WB is significantly more reliable than PPP in determining endotoxin concentration ex vivo. Measurement of endotoxin in PRP generated from WB was significantly diminished after 15 min, identifying a time frame within which to process blood samples for endotoxin analysis. 相似文献
Objective:
Objective: The purpose of this study was to determine the effect of blood sample fraction and handling time on measurement of endotoxin concentration in horses.
Methods:
Methods: Whole blood, anticoagulated with 3.8% (0.12 M) sodium citrate (9:1), was collected from 5 healthy horses. Whole blood (WB), platelet-rich plasma (PRP), and platelet-poor plasma (PPP) were spiked with endotoxin (2 EU/mL). Endotoxin-spiked WB samples were centrifuged immediately to generate PRP for measurement. Endotoxin concentration was subsequently measured by Limulus amebocyte assay at 0, 15, 30, 45, and 60 minutes. Assays were performed in triplicate and results were analyzed using Student's t -test, with significance set at P < .05.
Results:
Results: Mean endotoxin concentrations in 2 EU/mL-spiked WB were significantly different from those in PPP at all time points tested. Recovery of endotoxin in PRP generated from WB was significantly diminished after just 15 minutes.
Conclusion:
Conclusion: PRP generated from WB is significantly more reliable than PPP in determining endotoxin concentration ex vivo. Measurement of endotoxin in PRP generated from WB was significantly diminished after 15 min, identifying a time frame within which to process blood samples for endotoxin analysis. 相似文献