基于WSN的温室环境监测节点设计

设计了一种带扩展板的无线传感器网络监测节点,节点以CC2430为核心,采用ZigBee技术实现无线传感器网络自组网和监测数据的自动汇聚,实现了无线传输、自适应组网、自动发现新加节点和失效节点、传感器类型可扩展、实时密集监测等功能,且具有功耗小、成本低、精度高、稳定性好等优点。     

耐盐丰产小麦品种德抗961的耐盐生理机制

以山农215953(SN215953)为对照,室内200 mmol/L NaCl模拟盐胁迫条件,从幼苗生长和光合机构两个方面研究了德抗961的耐盐生理特性。结果表明:盐胁迫条件下德抗961(DK961)地上部生物量(鲜重、干重)、相对含水量均高于SN215953,而其株高显著大于SN215953,这有助于对细胞内的盐分进行稀释,即“稀盐”性,保护细胞质不受离子毒害。同时,德抗961叶片的希尔反应活力和Ca2+-ATPase活性受抑制的程度较小,光合能力相对较高。分析认为,以上两个方面可能是德抗961抗盐性强的重要原因。     

布鲁菌Ⅳ型分泌系统效应蛋白DK63-887基因缺失株的构建及鉴定

为了构建羊布鲁菌16M(简称16M)的DK63-887基因缺失株(16MΔDK63-887),探讨该基因与16M介导自噬的关系。利用同源重组和抗性替换的方法,以卡那基因替换DK63-887基因,获得突变株16MΔDK63-887。将亲本株16M、疫苗株M5-90、突变株16MΔDK63-887在相同条件下振荡培养,观察其生长趋势变化;将各菌株置于不同外界环境中,观察其生存率;将各菌株侵染小鼠巨噬细胞,比较它们在宿主细胞内的生存能力及RT-qPCR检测自噬相关基因的表达。成功获得了布鲁菌DK63-887基因缺失株且在20代内未发生回复性突变现象。与亲本株相比,16MΔDK63-887在体外培养生长趋势与亲本株相似,只是细菌的浓度存在一定差异;突变株在外界应激条件下生存能力低于亲本株;侵染4h后缺失株胞内细菌数量明显下降;RT-qPCR检测到突变株的ULKI、Beclin1表达量均显著降低(P0.01),结果表明,布鲁菌Ⅳ型分泌系统效应蛋白与16M介导的细胞自噬密切相关,为16M胞内寄生机制的研究奠定了基础。     

泰丰优656机插示范片高产栽培配套技术

介绍泰丰优656基本特征特性及近2 a在建宁县客坊乡多点试种、示范表现,总结泰丰优656作中稻实施机械化种植取得大面积高产的栽培技术。     

泰丰优656试种表现及栽培技术

泰丰优656是用泰丰A×福恢656配制而成的杂交水稻新组合,连续2年在福建省屏南县甘棠乡种植3333 m2,表现群体整齐、植株较高、穗大粒多、后期转色好、丰产性好,总结其种子处理、壮秧培育和大田高产栽培技术.     

Management of peanut pod rot I: Disease dynamics and sampling

Peanut fields are monitored for pod rot, which is typically caused by Pythium spp. and Rhizoctonia solani, in order to determine need, and the type and timing of fungicide applications. Pod rot can lead to damaged peanut kernels and when damage exceeds 2.49%, substantial price reductions occur. Nine fields or tests were sampled weekly for pod rot during the 2009 through 2012 growing seasons. The sampling was conducted on fields treated uniformly with fungicides for pod rot or within large research plots with various fungicide treatments. Pythium myriotylum was the most frequently identified pathogen species, although Rhizoctonia spp. were also recovered from diseased pods at all sites. Pod rot incidence was related to percent damaged kernels at harvest in 3 of 5 sites. Collection of 304 samples (sample unit = 46 cm of row) in a field was required to estimate 1% pod rot accurately (CV = 20%). There was a linear relationship between average % pod rot in a field, and the percentage of sampling units (absence/presence) with pod rot at low disease incidences. Scouting for pod rot of peanuts to make in-season fungicide applications will be hampered by high sample number, destructive sampling of plants, frequent sampling (due to rapid increase of disease), and the poor relationship between disease during the season and kernel damage at harvest. Making one preventative application at 60–70 days after planting may be a better practice than timing the initial fungicide application based on sampling for disease.     

美国玉米种DK743引种高产群体结构初探

研究了DK743玉米杂交种的密度效应.结果表明经济产量-密度方程呈渐近线型,经济系数-密度方程呈指数下降型.密度为67 500株/hm2的群体经济产量最高为13 300.33kg/hm2,收获穗数67500穗/hm2,穗粒数559粒,千粒重352.49 g,经济系数0.502 9,为最佳产量群体结构.     

助剂DK8对杀菌剂可湿性粉剂性能提高作用的研究

助剂DK8是我国企业开发的一种农药助剂,本文研究了助剂DK8对杀菌剂可湿性粉剂（WP）性能的提高作用,助剂DK8可以显著提高杀菌剂可湿性粉剂的悬浮率,降低润湿时间.活体组织法实验表明添加5％的DK8可提高福美双、菌核净、戊唑醇可湿性粉剂对油菜菌核病的防治效果.田间试验结果表明助剂DK8对菌核净防治油菜菌核病也有一定的促进作用.     

迪卡一号玉米最佳密度和施肥量试验

对迪卡一号玉米进行了不同密度和不同施氮量试验,结果表明,不同密度、不同施氮量条件下产量差异达极显著水平。综合产量和经济效益,以每公顷施522kg氮肥(尿素)为最佳,随着密度增加产量升高,最适宜的密度为每公顷64500株左右。