功能性inositol类物质在不同植物中的含量分析

利用乙醇抽提不同植物中的inositol类物质,将其抽提物中的水溶相部分依次通过ODS柱和阴离子交换柱后,用HPLC法进行分析。结果表明,D-pinitol含量多的植物有槐树叶、红花三叶草、白花三叶草、繁缕、银杏叶、婆婆指甲菜;D-chiro-inositol含量多的植物有蒲公英、白背;ononitol含量多的植物有槐树和婆婆指甲菜;myo-inositol含量多的植物有金银花、柿子叶、蒲公英、桑树叶。     

HPLC-ELSD测定豆科植物中D-松醇的含量

[目的]比较研究豆科植物中的D-松醇含量。[方法]采用高效液相色谱-蒸发光散射检测器（HPLC-ELSD）测定黄皮黄豆、绿皮黄豆、大豌豆、黑豆、红豆、豆角、荷兰豆、豌豆、毛豆、刀豆的D-松醇含量。[结果]D-松醇含量测定的标准曲线回归方程为：Y=1.421 1X＋6.276 4,r=0.826 5,其进样质量线性范围为0.377～1.844μg。青豌豆荚的D-松醇含量为0.084%,青豌豆子实的D-松醇含量为0.339%,青荷兰豆子的D-松醇含量为0.190%,青荷兰豆子实的D-松醇含量为0.342%,青毛豆荚的D-松醇含量为0.760%,青毛豆籽实的D-松醇含量为1.135%,青长豆角的D-松醇含量为0.203%,荷兰豆的D-松醇含量为0.342%,黄皮大豆的D-松醇含量为0.198%,绿皮大豆的D-松醇含量为0.229%,黑皮大豆的D-松醇含量为0.319%,赤豆、豌豆和刀豆中未检出D-松醇。[结论]D-松醇在豆科植物中广泛存在,不同生长期其含量差别较大。     

Variability and selection on different <Emphasis Type="Italic">Argyrolobium roseum</Emphasis> accessions for morphological traits and yield

Variability studies were carried out among different accessions of Argyrolobium roseum for 12 metric traits under natural and cultivated conditions. Almost all characters showed higher values in cultivated than natural population. Leaf breadth recorded highest CV (30.59%). Highest percentage of vitexin and D-pinitol (0.208 and 0.773% dwb) was observed in RAR-7 and RAR-6, respectively, under cultivated conditions. Phenotypic coefficient was higher than genotypic coefficient of variation. The association analysis revealed that root yield had positive significant correlation with 100 seed weight (r=0.839) biomass yield (r=0.601), where biomass yield had a positive significant correlation with leaf length, single pod weight. High heritability (97.4–99.8%) coupled with moderate genetic advance ranged between (49–67%) as a percent of mean was observed for leaf breadth, pod length, total foliage biomass yield and total root yield/plant. This suggests that direct selection for these traits is suitable for the improvement of this crop.     

3-O-甲基-1,2:5,6-二-O-异亚丙基-D-肌醇的高效合成试验

以天然产物多元醇D-Pinitol为原料,经与丙酮发生缩合反应,得到了两个异亚丙基保护的肌醇衍生物(2)和(3),试验得到了此反应的最佳条件,并通过红外光谱(IR)、核磁氢谱(1H-NMR)和核磁碳谱(13C-NMR)对产物的结构进行了确认.