‘哈伯’南天竹组织培养和快速繁殖

为建立‘哈伯’南天竹组织培养和种苗繁育技术体系,以半木质化带芽茎段为外植体材料开展植株再生研究。通过观察对比试验法、L₉(3⁴)正交试验设计完全随机法、极差分析、显著性检验、LSD多重比较,探讨了‘哈伯’南天竹组培的最适培养基配方。试验结果表明：最佳诱导培养基为MS + 6-BA 2.0 mg/L + IBA 0.1 mg/L +蔗糖30 g/L,诱导萌动率71.77%,成活率85.51%;最佳增殖培养基为WPM +6-BA 1.5 mg/L + IBA 0.01 mg/L + 蔗糖30 g/L,增殖系数6.3;最佳生根培养基为1/2 MS+ IBA 0.5 mg/L + NAA 1.0 mg/L + 蔗糖20 g/L + AC 0.2 g/L,生根率97.63%;试管苗移入泥炭土:珍珠岩=3:2(V/V)混合基质中,移栽成活率96.67%。该试验建立了高效稳定的组培快繁技术体系,得到的组培苗后代能够稳定的保持母本优良性状,为工厂化育苗提供了技术支撑。     

徐光启农书所载肥料与施肥技术研究

徐光启所著《农政全书》《徐光启手迹》二书中包含了大量有关肥料与施肥技术的内容,其中既有徐氏对明以前农书中提及的肥料技术经验的吸收,也有徐光启本人在前人基础上亲身实验得出的新的成果与感悟。从内容上看,与前代相比,徐氏书中所载的明代肥料种类有了很大增加,肥料主要成分也发生了明显变化。徐氏所总结的明代施肥技术具有因地制宜使用肥料、根据作物选择不同肥料、注意施肥适量、基肥与追肥搭配施肥等特点。     

旱地棉花主要促控措施的产量效应分析

本文根据三年试验结果所建立的数学模型,结合4—9月份的不同降雨量条件,对旱地棉花增加种植密度以后各种促控措施(施(?)量、施磷量、打顶日期、化控日期)的合理定量进行了分析。结果认为:早地棉花的高产栽培方案应以种植密度和磷肥的合理定量为基本內容,氮肥、打顶和化控作为依据天气情况和苗情酌情实施的调节措施。最后,依据试验结果,对方案的可行性及优点进行了讨论。     

NAA和几种细胞分裂素对怀山药离体快繁的影响

主要探讨了三种嘌呤型细胞分裂素:呋喃氨基嘌呤(KT)、苄氨基嘌呤(BAP)和异戊烯基腺嘌 呤(2ip),对怀山药的两个品种:淮山药和凤山药离体培养的影响。结果表明:两品种在MS KT2mg/L (单位下同)或2ip培养基上生长情况均较好,当附加NAA0．2时,一般可促进生根;但在培养基上,两 品种差异明显,淮山药表现腋芽生长受抑制,而凤山药则易形成丛生芽,而当此培养基附加NAA0．2 时,NAA与BAP可协同作用促进淮山药的腋芽生长,增加芽苗数,但对凤山药的生长则基本无影响。 通过不同培养基对凤山药快繁效果的比较,筛选出MS KT1 NAA0．2 0．1％活性碳为凤山药快繁较适 宜的培养基。     

Study on Cultural Technologies and Salt-resistance of Nitraria sibirica in Coastal Areas with Serious Salt-affected Soil

In this research, five Nitraria sibirica provenances such as Dayilu were selected to do experiment of salt-resistance, combining fixed plots test with typical plot investigation, measuring survival rate and growth as main indicators. The experiments of main cultural technology were done on Nitraria sibirica in 4 types of site preparation, 3 types of planting, 3 designs for planting density. The Nitraria sibirica experiments for the effects of curbing salt and improving soil were done by testing soil nutrients, salt content, soil physical properties as focal points in typical site of 3-4 years woods. The experiment results showed that the Nitraria sibirica could survive well with soil salt content of 0.6%, and that survival rate declined distinctively as soil salt content exceeded 0.8%. However, high soil salt content did not influence growth in the year of planting. There were obvious differences in salt-tolerance among Nitraria sibirica provenances; Dayilu was the best on salt-tolerance, the other 4 pro     

弘扬茶文化 促进社会主义和谐社会建设

中国是茶的故乡,是世界上茶历史最悠久、茶资源最丰富、茶文化最深厚的国家.茶在中国始终扮演着社会交往的最佳礼物和媒介,是人与人之间友谊的桥梁.茶文化的本质是"和谐文化",它引导人们与自然和谐相处,启示人们在人与人的关系上,要彼此尊重,加强交流,启发人们感悟身心的统一.大力弘扬茶文化精神,是建设和谐文化的一笔重要资源,是构建和谐社会的重要载体.     

水产养殖清洁生产的内涵与技术

清洁生产作为循环经济的主要生产方式适用于各生产性行业并有助于各生产行业的可持续发展。以陆上工厂化水产养殖为例，讨论了水产养殖业实施清洁生产的技术。     

科技是云南草地畜牧业可持续发展的关键因素

针对云南草地资源的现状及面临的问题，进行了草地畜牧业持续发展综合配套技术的研究，试验、示范、推广效果突出、效益明显，从而提出了科技是草地畜牧业可持续发展的基础和关键因素，以及加快科技推广的一系列建议。     

Rapid Screening for Aluminum Tolerance in Cereals by Use of the Chlorophyll Fluorescence Test

The effects of aluminum on the photosynthetic apparatus were examined in cereals grown in nutrient solutions (pH 4.5) at two Al levels (0 and 148 μM). The chlorophyll fluorescence kinetics results confirmed that the soft wheat ‘BHG’ cultivar has the potential for growth on acid soils while triticale cultivars ‘Niovi’ and ‘Dada’ appeared to be relatively tolerant. The percentage decrease in F_v/F_m of the less tolerant cultivars after Al-treatment indicated a decrease in the efficiency of the primary photochemistry of PS II, while the decrease in the ratio F_V/F_osuggested that exposure of the cultivars ‘Dio’ and ‘Appulo E’ to aluminum caused injury to the thylakoid structure. The percentage fluctuations of the ratio F_v/F_mwere shown to correlate very closely with the assessment of injury as evaluated by the relative top fresh weight. Measurements of chlorophyll fluorescence in vivo could be used to monitor injury caused by “Al-stress”, and thus they may serve as a rapid screening test for Al tolerance.     

糖和氮对山楂组织培养新梢生根的影响

采用敞口山楂组培新梢研究糖和无机氮源对不定根形成的影响,含有高水平蔗糖的培养基比低水平蔗糖的培养基新梢生根率高。新梢对糖的这种反应与糖代谢和物理渗透都有关,用甘露醇作为渗透代替剂在低浓度蔗糖存在下无作用,当蔗糖浓度达到一定程度时(86.64mmol·1~(-1))能影响新梢的生根率。在培养基中分别加入NO_3~(-)-N或NH_4~(+)-N作为唯一氮源,或是在MS培养基中加入NO_3~-/NH_4~+比率为标准的混合氮源,在测试范围(0～120mmol·1~(-1))内均降低新梢的生根率,三者对生根的抑制作用大小顺序依次为NH_4~+>NO_3~(-)-NH_4~(+)-N>NO_3~(-)-N.NO_3~-/NH_4~+的比率影响生根。NO_3~-/NH_4~+比率与生根之间呈直线正相关。糖/氮比值高有利于生根。插穗中碳、氮的绝对量及碳/氮比率可作为山楂新梢生根的指标。