山羊实验感染伊氏锥虫病理学研究

本文研究了山羊实验感染伊氏锥虫后的血液成分和病理学变化。结果表明:血清中的钾和血糖含量均下降,红细胞数和血红蛋白亦下降,而谷草转氨酶和白细胞数均升高;血清白蛋白、β球蛋白含量下降,γ球蛋白升高,血清总蛋白和血清谷丙转氨酶则无明显变化。组织病理学变化可见支气管肺炎及间质性肺炎、增生性脾炎、肾空泡变性、脑水肿,心肌空泡变性、肝脂肪变性、单纯性淋巴结炎、肾上腺和胰腺空泡变性及慢性肠卡他。     

The clinicopathologic significance of enriching grated cassava mash with red palm oil in the production of gari

The neglect of traditional processing methods in the production of gari (toasted cassava granules) has been blamed for reported cases of poisoning following consumption of gari. This study investigatedthe nutritional and clinicopathologic effects of not enriching cassavamash with red palm oil (RPO) during gari production. Two garisamples were produced with and without RPO. Total cyanogen, acetonecyanohydrin, free cyanogen and crude protein contents were not found tobe significantly (p>0.05) different between the two. The samples werefed exclusively to two different groups of Sprague-Dawley rats for a tenweek experimental period during which clinical observations were recordeddaily. At the end, vital body organs were examined grossly and microscopically. There was a significant (p<0.05) reduction in severityand percentage of animals exhibiting clinical abnormalities and lesions ofchronic cyanide poisoning in the group fed gari produced with RPO.This result implies an association between the enrichment of cassava mashwith RPO during gari production and the reduction of severity andpercentage of animals affected by chronic cyanide poisoning.     

Expression of Foxp3+ Tregs and PD1 in gastric cancer tissues and their correlation with clinicopathological factors and prognosis

AIM: To investigate the expression of Foxp3⁺ regulatory T cells (Foxp3⁺ Tregs) and programmed death receptor 1 (PD1) in gastric cancer tissues and their association with clinicopathological factors and prognosis of the patients. The correlation between the 2 molecules was also analyzed at the same time. METHODS: The tumor sections from 111 gastric cancer patients were stained for Foxp3 and PD1 by the method of immunohistochemistry. The associations of the expression levels of these 2 molecules with clinicopathological factors involved in the disease progression and prognosis were statistically analyzed. The relationship of their expression was detected. RESULTS: Foxp3⁺ Tregs and PD1 were expressed in the gastric cancer tissues, and PD1 was expressed in the tumor infiltrating lymphocytes (TILs). The expression of Foxp3 and PD1 was correlated with lymph node metastasis, clinicopathological stage and prognosis of gastric cancer patients. The expression of these 2 determinants in the patients with lymph node metastasis and an advanced clinicopathological stage was distinctly higher (P <0.05). The patients with positive expression of the 2 indexes presented a lower overall survival rate and worse prognosis (P <0.05). A significantly positive correlation between the infiltration of Foxp3⁺ Tregs and the expression of PD1⁺ TILs was also observed (P <0.01).CONCLUSION: Foxp3⁺ Tregs and PD1⁺ TILs co-infiltrate in the gastric cancer tissues, which can be used as biological markers to predict the disease progression and prognosis.