乌龟肌肉营养价值的评定

分析结果表明,乌龟的水分、总糖、灰分含量分别为80 24%,0 66%和0 95%。乌龟肌肉的蛋白质含量达16 64%,必需氨基酸和鲜味氨基酸分别占氨基酸总量的49 16%和43 39%,氨基酸组成中以Glu含量最为丰富,Ie是第一限制性氨基酸,氨基酸分为82 63;乌龟肌肉脂肪含量为1 51%,脂肪酸组成以C18∶1为主,达39.32%,其次为C16∶0及C22∶6,不饱和脂肪酸占脂肪酸总量的76 83%。     

乌龟鲍曼不动杆菌的分离鉴定及药敏试验

自患病乌龟肝脏中分离出1株优势菌DL01221,对其进行形态学观察、生化试验、16SrRNA基因序列分析,同时采用Kriby-Bauer纸片扩散法进行药物敏感性试验。试验结果表明,该菌为革兰氏阴性球杆菌,将该菌株16SrRNA序列进行同源性和系统进化树分析,发现它与鲍曼不动杆菌处于同一群,同源性超过98%,结合菌株形态和生化指标,确定该菌株为鲍曼不动杆菌。致病性试验结果显示,其对乌龟具有较强的致病作用;耐药性结果显示其对头孢他啶、卡那霉素及氟罗沙星敏感,对其他多种抗生素耐药。     

Assessment of shoreline vegetation in relation to use by molting black brant Branta bernicla nigricans on the Alaska coastal plain

To evaluate the importance of large thaw lakes on the Alaska Coastal Plain for molting Pacific black brant Branta bernicla nigricans, distribution and life form of shoreline vegetation were assessed using several scales: satellite imagery, point-intercept transects, cover quadrats, and a parameter for water regime. Brant population and distribution estimates from aerial surveys were used to classify large lakes into high, moderate, and low use. Correlations between brant and abundance of their preferred feeding site — moss flats — were best demostrated by satellite imagery. Intercepts and cover ratings were not correlated, presumably because these techniques were less efficient at assessing area. General observations suggested that the presence of islands, large ice floes, and possibly other physical attributes of the habitat, influenced brant distribution. This area is unique because of low-lying, drained-lake basins that have ideal combinations of moss flats and large water areas where brant seek protection disturbance is vital to the success of this declining species because alternate habitats may not be available elsewhere on the Coastal Plain. in water or on ice floes. Protection of the area from     

石河子地区鲜食葡萄烂果病病原鉴定

[目的]明确石河子地区鲜食葡萄果实烂果病的主要病原.[方法]通过田间调查及对具有典型烂果症状的病样进行分离纯化和培养,对病原进行常规形态学和分子生物学鉴定.[结果]通过鉴定查明引起石河子地区葡萄烂果的病原菌主要有三种:灰葡萄孢菌(Botrytis cinerea .Pers),黑曲霉(Aspergillus niger)和黑根霉(Rhizopus nigricans).[结论]石河子葡萄烂果的主要病原有3种,其诱因主要与8月上旬久旱遇雨和膨大剂用量较多有关.     

1株草龟维氏气单胞菌的分离鉴定及药敏谱分析

旨在确定河北昌黎县某花鸟鱼店宠物草龟发病死亡的病因,笔者从发病草龟体内分离纯化出1株优势菌CLW-1,通过解剖观察、细菌分离培养、革兰染色镜检、生化鉴定和16S rDNA基因测序对其分析鉴定,并通过药物敏感性测试检测菌株的耐药性,通过斑马鱼接种试验和毒力基因检测其致病性。结果显示,该菌在血琼脂平板形成湿润、表面光滑、呈β溶血的灰白色菌落,在RS琼脂培养基上长出光滑湿润的黄色菌落;革兰染色镜检为阴性短小球杆菌;16S rDNA基因序列同源性分析表明,分离菌株CLW-1与维氏气单胞菌为同一族,相似性均达99%以上;斑马鱼接种试验结果显示,该菌具有较高的致病力,其LD₅₀为1.26×10⁶CFU;毒力基因扩增结果表明,分离菌株CLW-1具有aerA、Exu、Lip和Ser毒力基因;药敏试验结果显示,分离菌株CLW-1对哌拉西林、头孢唑林和恩诺沙星等敏感;对呋喃唑酮、苯唑西林和红霉素等中敏;对麦迪霉素、克林霉素和万古霉素等耐药。通过试验结果确定此分离菌株CLW-1为维氏气单胞菌,携带毒力基因,具有较高致病力,应用对应的抗生素治疗效果较好,为维氏气单胞菌病的防治奠定基础。     

响应面法优化黑盖木层孔菌菌丝体液体发酵培养基研究

应用Plackett-Burman设计法对影响液体发酵黑盖木层孔菌菌丝体产量的培养基组分进行筛选,确定影响菌丝体产量的关键因素为玉米粉、麸皮和VB1。在此基础上,采用最陡爬坡试验结合Box-Behnken响应面法优化黑盖木层孔菌液体发酵培养基。结果表明:当培养基组分中玉米粉为65 g/L、麸皮为49.31 g/L、VB1为214.61μg/L时,菌丝体产量预测值为10.75 g/L,最佳条件下菌丝体产量可达11.16 g/L,预测值与验证值吻合得较好。     

巴西彩龟对温和气单胞菌的免疫应答研究

选择对巴西彩龟强毒力的温和气单胞菌菌株(AB98043),扩大培养制成灭活福尔马林菌苗,分别对巴西彩龟进行注射和浸泡试验。经过对吞噬细胞吞噬活性、血清抗体效价的检测和攻毒试验,结果表明,受免组各项指标均明显高于对照组,免疫保护力达到82%以上,其中浸泡组各项指标略低于注射组。     

Isolation,Identification and Drug Sensitivity Test of a Strain of Aeromonas veronii from Chinemys reevesii

To determine the cause of death of Chinemys reevesii in a pet shop in Changli county, Hebei province, a dominant strain CLW-1 was isolated and purified from the diseased Chinemys reevesii. It was analyzed and identified through anatomical observation, bacterial isolation and culture, Gram staining and microscopic examination, biochemical identification and 16S rDNA gene sequencing, the drug resistance of the strain was detected through drug sensitivity test, and its pathogenicity was detected through zebrafish inoculation test and virulence gene experiment. The results showed that the bacteria formed moist, smooth surface, gray colony with β hemolysis on blood agar medium, and grew smooth and moist yellow colony on RS agar medium; Gram staining and microscopic examination showed negative coccidiosis; Homology analysis of 16S rDNA gene sequence showed that the isolated strain CLW-1 belongs to the same group with Aeromonas veronii, and the homology is above 99%; Inoculation test results of zebrafish showed that the strain had high pathogenicity, and its LD₅₀ was 1.26×10⁶ CFU; The virulence gene amplification results showed that the isolated strain CLW-1 had virulence genes of aerA, Exu, Lip and Ser; Drug sensitivity test results showed that the isolated strain CLW-1 was sensitive to piperacillin, cefazolin and enrofloxacin, etc; Susceptibility to furazolidone, oxacillin and erythromycin, etc; Resistance to midecamycin, clindamycin and vancomycin, etc. According to the test results, the isolated strain CLW-1 is confirmed to be Aeromonas veronii, which carries virulence genes and has high pathogenicity, it has great therapeutic effect by applying corresponding antibiotics, thus laying a foundation for the prevention and treatment of Aeromonas veronii.     

中国松塔牛肝菌属,假黑伞属和黑伞属的新记录种

记述在海南岛发现的中国松塔牛肝菌属、假黑伞属和黑伞属的新记录种角鳞松塔牛肝菌(Strobilomyces foveatus Corner.)、黄褐球盖菇(Stropharia coronilla Bull.)和卷鳞灰黑菇(Agricus meleagris J.Schaeffer),以及我国未详细报道的疣鳞松塔牛肝菌(Strobilomyces nigricans Berk.)和刺疣松塔牛肝菌(S.confusus、Sing.)的形态。     

广西草龟GH基因全序列克隆及测序分析

[目的]了解广西草龟生长激素(GH)基因全序列结构特点及其与其他物种间的关系,为进一步研究GH基因的结构、功能、遗传变异规律及其与生产性能的关系提供科学依据.[方法]根据GenBank上已公布的黄喉拟水龟GH基因序列设计合成9对特异性引物,以广西草龟基因组DNA为模板进行PCR扩增,所获得目的片段经纯化、克隆、鉴定和测序后,用Vector NTI Advance 10软件将各扩增序列拼接,获得草龟生长激素基因全序列,并用DNASTAR软件MegAlign程序中的Alignment Report和NCBI的BLAST对草龟GH基因全序列进行同源性比对分析及构建基于GH基因编码序列的物种间亲缘进化树.[结果]广西草龟GH基因全长8538 bp,与黄喉拟水龟GH基因全长8517 bp相比,二者的同源性高达98%,其中有118个碱基发生突变,插入37个碱基,14个位点发生碱基缺失;与鸡、鸭、鹌鹑、鸵鸟、红树林蛇的同源性分别为88%、83%、87%、86%和84%.[结论]GH基因编码序列具有较高的保守性.