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为给葱的染色体的识别提供新标记,建立葱的分子细胞遗传学核型,本研究采用去壁火焰干燥法制备了分散且形态良好的葱中期染色体,并进行了CPD(PI和DAPI组合)染色和45S rDNA荧光原位杂交(FISH),根据葱染色体的形态特征,结合CPD染色和FISH结果,对葱进行了核型分析。CPD染色结果:葱所有染色体臂末端都显示CPD带。FISH结果:有一对45S rDNA位点(在第5对染色体上)。葱的核型公式:2n=2x=16=2sm+12m+2st(SAT)。研究表明:利用CPD染色和45S rDNA FISH,不仅能为染色体识别提供新标记,还能了解染色体GC丰富区的分布,为葱属植物的物种鉴定、系统分类与进化等研究提供DNA分子方面的证据。 相似文献
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Lori S. Waddell DVM DACVECC David E. Holt BVSC DACVS Dez Hughes BVSc MRCVS DACVECC Urs Giger PD DR. med. vet. DACVIM 《Journal of Veterinary Emergency and Critical Care》2001,11(1):23-26
Objective: To determine the effect of storage on ammonia concentration in canine packed red blood cell (pRBC) units.
Design: In vitro and in vivo study.
Setting: University Veterinary Teaching Hospital.
Interventions: Ammonia concentration was measured in 7 units of canine pRBC prepared in citrate-phosphate-dextrose (CPD) and Adsola on Days 1 and 35 of storage. Ammonia was measured in 4 additional units of canine pRBC on Days 0, 7, 14, 21, 28, and 35. Plasma ammonia was also determined in 5 anemic dogs receiving pRBC.
Measurements and Main Results: Ammonia concentration increased from 73 ± 15 mmol/L (mean ± SD) on Day 1 to 800 ± 275 mmpl/L on Day (p<0.001). When measured every 7 days in 4 units of canine pRBC, ammonia concentration increased from 23 ± 8 mmol/L on Day 0 to 179 ± 13 mmol/L (Day 7), 276 ± 56 mmol/L (Day 14). 383 ± 47 mmol/L (Day21), 466 ± 30 mmol/L (Day 28), and 562 ± 27 mmol/L (Day 35) (p<0.05 for all comparisons). In a preliminary study, plasma ammonia concentration measured in blood samples from 5 anemic dogs without primary liver disease immediately before and after transfusion with 5–10 ml/kg of stored pRBC remained in the normal reference range.
Conclusions: The ammonia concentration in stored canine pRBC increased markedly with time. In this preliminary study, ammonia concentrations in dogs without primary liver disease did not increase above the reference range after transfusion with pRBC. 相似文献
Design: In vitro and in vivo study.
Setting: University Veterinary Teaching Hospital.
Interventions: Ammonia concentration was measured in 7 units of canine pRBC prepared in citrate-phosphate-dextrose (CPD) and Adsol
Measurements and Main Results: Ammonia concentration increased from 73 ± 15 mmol/L (mean ± SD) on Day 1 to 800 ± 275 mmpl/L on Day (p<0.001). When measured every 7 days in 4 units of canine pRBC, ammonia concentration increased from 23 ± 8 mmol/L on Day 0 to 179 ± 13 mmol/L (Day 7), 276 ± 56 mmol/L (Day 14). 383 ± 47 mmol/L (Day21), 466 ± 30 mmol/L (Day 28), and 562 ± 27 mmol/L (Day 35) (p<0.05 for all comparisons). In a preliminary study, plasma ammonia concentration measured in blood samples from 5 anemic dogs without primary liver disease immediately before and after transfusion with 5–10 ml/kg of stored pRBC remained in the normal reference range.
Conclusions: The ammonia concentration in stored canine pRBC increased markedly with time. In this preliminary study, ammonia concentrations in dogs without primary liver disease did not increase above the reference range after transfusion with pRBC. 相似文献
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尼龙袋法评定鹿常用饲料的营养价值 总被引:6,自引:1,他引:5
用3头装有永久性瘤胃瘘管的成年公梅花鹿,采用尼龙袋法,评定了49种73个样品鹿常用饲料的干物质有效降解率(DMD,下同),蛋白质有效降解率(CPD,下同),有机物有效降解率(OMD,下同)。结果表明,尼龙袋法能较好地评定梅花鹿饲料的DMD、CPD、OMD和反映饲料品质差异;同类不同种饲料及同种饲料因收获期不同、处理不同、部位不同其DMD、CPD、OMD差异较大;同种不同产地饲料间的DMD、CPD亦有差别。 相似文献
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莪术CPD染色和45S rDNA荧光原位杂交核型分析 总被引:1,自引:0,他引:1
为了对莪术[Curcuma zedoaria (Christm.) Roscoe]的染色体进行识别并对该物种基因组的结构及进化进行初步研究,利用改进的火焰干燥法及荧光原位杂交技术,对莪术中期染色体的长度,着丝粒的位置及随体的数目进行分析。PI和DAPI组合(CPD)染色后和相继的45S rDNA探针荧光原位杂交结果显示,莪术具有五对45S rDNA位点,三对位于8,22,31号染色体末端的CPD带区,二对位于4,30号染色体的短臂上。第五号短臂为富含GC对的非45S rDNA位点。该实验建立了莪术的经典核型,为非整倍体,核型公式为2n=62+1=40m+12sm+1m,其核型不对称性为2A型。 相似文献
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Chris Wong DVM Steve C. Haskins DVM MS DACVECC DACVA 《Journal of Veterinary Emergency and Critical Care》2007,17(1):32-36
Objective: To determine the effect of storage on the P50 of feline hemoglobin. Design: Prospective, in vitro, laboratory study. Subjects: Venous blood from 4 clinically healthy cats. Measurements: Blood was collected into CPDA‐1 anticoagulant/preservative and maintained at 4°C for 5 weeks. Measurements were made on Days 0, 2, 4, 7, 14, 21, 28, and 35. The blood samples were equilibrated in a tonometer to gas mixtures containing 2.5%, 4%, 5%, or 8% oxygen, with 5% carbon dioxide balance nitrogen; pH was adjusted to 7.4. Chloride, partial pressure of oxygen, and hemoglobin saturation were measured; P50 was calculated. Results: Chloride decreased from 124.3±2.1 to 88.5±1.9 mEq/L immediately after dilution with CPDA‐1, and did not change for the 5 weeks thereafter. The P50 decreased from an average of 35.0±1.2 to between 31 and 32 mmHg after 7 days, and did not change further for 4 weeks thereafter. Conclusions: The decrease in P50 of feline hemoglobin was minor compared with that of blood from species in which 2,3‐diphosphoglycerate (2,3‐DPG) is a major modifier of hemoglobin affinity for oxygen. The decrease in P50 in the present study was attributed to an initial decrease in chloride and a subsequent loss of modest quantities of red cell 2,3‐DPG. 相似文献
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[目的]研究大葱的CPD带型,为大葱的染色体识别提供标记,了解大葱染色体DNA序列的分子特征。[方法]采用去壁火焰干燥法制备大葱的根尖细胞有丝分裂染色体,用CPD(PI和DAPI组合)对大葱染色体进行染色,结合常规的染色体测量,对大葱进行核型分析。[结果]CPD染色后,大葱的所有染色体的端点区都显示了红色的CPD带;大葱的核型公式为2n=2x=16=14m+2st(SAT)。[结论]根据CPD带,可对大葱的染色体进行更加准确的识别;大葱的所有染色体的端点区都含有GC丰富的DNA序列;CPD染色可为葱属植物的物种鉴定、系统分类与进化等方面的研究提供DNA分子方面的证据。 相似文献
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