全文获取类型
收费全文 | 2885篇 |
免费 | 157篇 |
国内免费 | 288篇 |
专业分类
林业 | 150篇 |
农学 | 243篇 |
基础科学 | 27篇 |
246篇 | |
综合类 | 910篇 |
农作物 | 317篇 |
水产渔业 | 248篇 |
畜牧兽医 | 844篇 |
园艺 | 158篇 |
植物保护 | 187篇 |
出版年
2024年 | 4篇 |
2023年 | 39篇 |
2022年 | 80篇 |
2021年 | 97篇 |
2020年 | 81篇 |
2019年 | 100篇 |
2018年 | 61篇 |
2017年 | 113篇 |
2016年 | 122篇 |
2015年 | 148篇 |
2014年 | 171篇 |
2013年 | 161篇 |
2012年 | 211篇 |
2011年 | 217篇 |
2010年 | 167篇 |
2009年 | 207篇 |
2008年 | 161篇 |
2007年 | 190篇 |
2006年 | 166篇 |
2005年 | 120篇 |
2004年 | 110篇 |
2003年 | 74篇 |
2002年 | 59篇 |
2001年 | 63篇 |
2000年 | 73篇 |
1999年 | 39篇 |
1998年 | 35篇 |
1997年 | 27篇 |
1996年 | 38篇 |
1995年 | 26篇 |
1994年 | 27篇 |
1993年 | 19篇 |
1992年 | 23篇 |
1991年 | 20篇 |
1990年 | 14篇 |
1989年 | 19篇 |
1988年 | 8篇 |
1987年 | 9篇 |
1986年 | 3篇 |
1985年 | 4篇 |
1984年 | 3篇 |
1983年 | 4篇 |
1982年 | 5篇 |
1981年 | 2篇 |
1980年 | 2篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1975年 | 2篇 |
1963年 | 1篇 |
1955年 | 1篇 |
排序方式: 共有3330条查询结果,搜索用时 31 毫秒
1.
Cuticular protein gene LmACP8 is involved in wing morphogenesis in the migratory locust,Locusta migratoria 下载免费PDF全文
Cuticular proteins(CPs) are major components of the insect cuticle-associated organs such as integument and wings, although the importance of CPs for wing development and function in hemimetabolous insects remains understudied. In the present study, a wing cuticular protein LmACP8 was identified from Locusta migratoria, which belongs to the RR-2 subfamily of cuticular protein RR consensus(CPR) chitin-binding proteins. LmACP8 was mainly expressed in the wing pads and showed high expression levels before ecdysis of third-, fourth-, and fifth-instar nymphs, with its encoded protein located in the procuticle of wing pads and adult wings. Depletion of LmACP8 by RNA interference markedly reduced the amount of its protein, which consequently caused abnormal wing morphogenesis in the transition from nymph to adult of L. migratoria. We further demonstrated that the abnormal morphogenesis was caused by severe damage of the endocuticle in the wings. LmACP8 was suppressed by 20-hydroxyecdysone(20 E) in vivo, however, its expression was significantly up-regulated after knocking down the hormone receptor gene LmHR39. Thus, the LmACP8 that is negatively regulated by the LmHR39-mediated 20 E signaling pathway is involved in wing development during the nymph to adult transition. 相似文献
2.
Replacing Soybean Meal with Alternative Protein Sources in Diets for Pond‐raised Hybrid Catfish, ♀ Ictalurus punctatus × ♂ Ictalurus furcatus 下载免费PDF全文
Menghe H. Li Brian G. Bosworth Penelope M. Lucas 《Journal of the World Aquaculture Society》2018,49(4):755-760
The present study investigated the replacement of soybean meal with combinations of two or three alternative protein sources in diets for pond‐raised hybrid catfish, ♀ Ictalurus punctatus × ♂ Ictalurus furcatus. Alternative protein sources evaluated included cottonseed meal, distillers dried grains with solubles (DDGS), peanut meal, and porcine meat and bone meal (PMBM). Hybrid catfish fingerlings with a mean initial weight of 35 g/fish were stocked into 25 earthen ponds (0.04 ha) at a density of 14,826 fish/ha. Fish were fed once daily to apparent satiation for 166 d. No significant differences were observed for total diet fed, net yield, weight gain, survival, carcass yield, fillet yield, or fillet proximate composition among dietary treatments. Results show soybean meal may be completely replaced by combinations of cottonseed meal and one or two other alternative protein sources including DDGS, peanut meal, and PMBM in the diet without markedly affecting production and processing characteristics and fillet proximate composition of pond‐raised hybrid catfish. These alternative diets may be used during foodfish production when prices are favorable. 相似文献
3.
Margaret C. McEntee DVM Donald E. Thrall DVM PhD 《Veterinary radiology & ultrasound》2001,42(3):221-225
Twenty two dogs with an infiltrative lipoma had computed tomographic (CT) images acquired to evaluate the extent of local disease. Ten dogs had undergone at least one cytoreductive surgical procedure (range = 1-3; median = 2) prior to imaging. Twenty dogs had measurable disease on CT images; 2 dogs had diffuse disease at a previous surgical site that could not be measured. Tumor volume (n = 20) ranged from 20 to 5,632 cm3 (median = 345 cm3; mean = 996 cm3). None of the dogs had evidence of bone involvement on the CT images; 2 of the 22 dogs had tumors that did not come into direct contact with osseous structures. All dogs with measurable disease had evidence of a fat opacity mass with variable degrees of muscle infiltration. Eleven of 22 dogs were given intravenous contrast medium prior to image acquisition and there was not evidence of enhancement of the infiltrative lipoma in any dog. Based on CT images, tumors were classified as well-defined in 9 dogs, moderately well-defined in 4, not well-defined in 3 and a mix of well-defined and not well-defined in 6 dogs. Tumors tended to be less well-defined in regions where the infiltrative lipoma interdigitated with normal body fat. It appears CT imaging allows adequate discrimination of tumor with the caveat that differentiation of normal fat from infiltrative lipoma can be problematic. 相似文献
4.
经过蛋白质着色动力学的研究,发现蛋白质着色的行为遵循数学模型Y=aX^b。在pH2-6范围内,蛋白质上染料随染溶pH的下降而上升,在15-30℃温度范围内,蛋白质上染率随温度上升略有提高,当温度超过30℃时,蛋白质上染率逐渐下降。染浴中添加稀土对蛋白质有较强的促染效果,其实质是染浴中蛋白质首先对稀土高价阳离子进行结合,再通过高价阳离子对色素进吸附。 相似文献
5.
青海血蜱不同发育阶段蛋白分析 总被引:2,自引:0,他引:2
考虑到不同发育阶段蛋白多肽及抗原特性方面的差异,本实验利用SDS-PAGE和Westernblotting对青海血蜱的卵、幼蜱、若蜱、成蜱的蛋白进行了分析。SDS-PAGE结果显示,卵出现了7条主带,幼蜱出现了16条主带,若蜱出现了14条主带,成蜱出现了12条主带。它们的蛋白多肽在数量、含量和分子量上存在差异,共同带只有4条,但相邻发育阶段之间的共同带较多。用感染羊血清和免疫羊血清作免疫印迹时,感染羊血清在分子量5.6万处有明显的反应带,免疫羊血清在分子量9.7万处有明显的反应带。 相似文献
6.
甘肃滩羊血红蛋白(Hb)及红细胞蛋白质(Ep)多态性的研究 总被引:6,自引:3,他引:3
运用聚丙烯酰胺凝胶电泳技术(PAGE)对甘肃滩羊产区皋兰、景泰及靖远3县285只滩羊的血红蛋白(Hb)基因座、红细胞蛋白质-1(Ep-1)和红细胞蛋白质-2(Ep-2)基因座多态性进行了检测,结果发现甘肃滩羊Hb基因座存在3种基因型HbAA、HbAB、HbBB,它们受HbA和HbB两个共显性等位基因控制,其中HbB和HbBB在3个群体中均占优势;本研究未在Ep-1和Ep-2基因座上检测到多态性,它们均呈现单态。 相似文献
7.
8.
9.
浙农大A系意蜂生产性能考察试验报告 总被引:5,自引:0,他引:5
用杂交一代蜂群进行对比试验的结果表明,浙农大 A 系意蜂的产育力、群势增长速度、采蜜量等,与平湖意蜂和澳大利亚意蜂无显著差异或基本一致,但蜂王浆高产性能表现突出,产量比平湖意蜂高19.5%,比澳意高42.5%,而且质量指标符合国家规定和出口标准。 相似文献
10.
将马立克氏病死鸡的皮肤或羽囊捣碎,制成MD琼扩抗原。试验结果表明,自制MD皮肤和羽囊琼扩抗原与标准抗的检测MD抗体的敏感性和特异性相同,但皮肤琼扩抗原制造方法相对比较简单,产量也较高。 相似文献