翻白草乙酸乙脂提取液抗糖尿病功能性研究

[目的]研究翻白草乙酸乙脂提取液对糖尿病小鼠的降血糖功效。[方法]分5个试验组,即空白对照组,阴性对照组,阳性对照组灌胃盐酸二甲双胍100mg/kg,高剂量组灌胃翻白草乙酸乙脂提取液50g/kg,低剂量组灌胃翻白草乙酸乙脂提取液25g/kg。连续灌胃7d,于第8天禁食12h后断尾测血糖。[结果]翻白草高剂量（50g/kg）和低剂量（25g/kg）对四氧嘧啶所致糖尿病小鼠具有明显的治疗效果,但两者差异不大;但对正常小鼠血糖无显著影响。[结论]翻白草2个剂量组对糖尿病小鼠有明显的降血糖作用。     

美洲大蠊提取物对四氧嘧啶性糖尿病小鼠作用研究

[目的]研究美洲大蠊(MZDL)提取物对四氧嘧啶性糖尿病小鼠血糖的影响。[方法]四氧嘧啶诱发糖尿病小鼠后,随机分为模型组(MC),二甲双胍组(250 mg/kg),美洲大蠊提取物低剂量(25 mg/kg)、中剂量(50 mg/kg)、高剂量(100 mg/kg)5个组,模型组给予同体积生理盐水,分别于试验第7、14、212、8天各测定小鼠空腹血糖和体重,第25～28天每天记录进食饮水量。28 d后取血测血清中一氧化氮合酶(NOS)含量。[结果]3个剂量的美洲大蠊提取物均能减缓糖尿病小鼠消瘦和多饮多食的症状。在试验中中、高剂量组糖尿病小鼠血糖降低(P<0.05)。MZDL中、高剂量组NOS含量低于MG(P<0.01)。[结论]美洲大蠊提取物对四氧嘧啶致糖尿病小鼠具有一定的保护作用,其机制可能与其降低一氧化氮合酶水平有关。     

大红袍茶降血糖作用研究

[目的]探讨大红袍茶的降血糖作用。[方法]取50只小鼠以200 mg/kg剂量、间日2次腹腔注射四氧嘧啶制造高血糖模型,4 d后得到24只血糖高于9.00 mmol/L的高血糖模型小鼠。将高血糖小鼠按相似血糖水平分为对照组和试验组,血糖值分别为（15.11±6.47）和（14.77±5.40）mmol/L,组间无显著差异（P〉0.05）。对照组饲喂基础饲料和凉开水,试验组在基础饲料中添加3.0%的大红袍茶,饮0.5%的大红袍茶汤。饲养7 d后再次测定血糖值,研究其变化。[结果]对照组小鼠血糖值为（12.23±5.11）mmol/L,无显著变化（P〉0.05）;试验组小鼠血糖值下降到（7.93±1.88）mmol/L,降低了46.3%,变化极显著（P〈0.001）。[结论]大红袍茶对高血糖小鼠有极显著的降糖作用。     

金耳发酵液对糖尿病小鼠降血糖作用的研究

研究表明，金耳发酵液对四氧嘧啶诱导的糖尿病小鼠有降血糖作用，同模型对照组相比．大剂量组差异显著（P〈0.05）；同时，大剂量组对糖尿病小鼠摄食、饮水量的减少及体重的增加均有显著影响（P〈0.05）     

桑叶颗粒剂对糖尿病的治疗作用研究

为研究桑叶颗粒剂主剂对糖尿病的治疗作用,以四氧嘧啶诱导的糖尿病小鼠为实验动物进行了疗效研究。饲喂含1％和5％桑叶颗粒剂主剂的饲料,调查小鼠的临床表现及血糖值,结果表明,桑叶颗粒剂主剂可改善糖尿病小鼠“多饮”和体重降低的症状,1％和5％的主剂剂量均可极显著降低血糖值(P<0.01),5％的主剂剂量组即使停药后仍保持显著的降糖效果(P<0.05)。因此,桑叶颗粒剂对糖尿病小鼠有显著治疗作用。     

柞蚕雄蛾粉降血糖效果的初步研究

研究了柞蚕雄蛾粉对实验鼠的降血糖效果。结果表明:用添加4%的柞蚕雄蛾粉饲料饲喂高血糖鼠7 d后,雄小鼠血糖值降低23.1%,达极显著水平(p<0.01);雌小鼠血糖值降低10.4%,达显著水平(p<0.05)。表明柞蚕雄蛾粉具有降血糖作用。     

Feeding-induced increases in insulin do not suppress secretion of growth hormone

Secretion of growth hormone (GH) is reduced for several hours after feeding when access to feed is restricted to a 2-hr period each day. We hypothesized that increased secretion of insulin after feeding inhibits release of GH from the anterior pituitary gland. Our objectives were to determine whether: 1) alloxan prevents concentrations of insulin from increasing after feeding steers; 2) concentrations of GH remain high after feeding alloxan-treated steers; and 3) GH-releasing hormone (GHRH) stimulates greater release of GH in alloxan-treated, than in control, steers after feeding. Steers were injected iv with either saline (control) or with alloxan (110 mg/kg) (n = 4 per group). Concentrations of insulin were not different (P = 0.61) between control and alloxan-treated steers before feeding (87.5 +/- 33.6 pmol/l). However, alloxan prevented insulin from increasing (P < 0.001) after feeding (131.8 pmol/1) compared with control steers (442.0 pmol/l) (pooled SEM = 47.5). Overall, GH was higher (P < 0.05) in alloxan-treated (6.4 ng/ml) than in control steers (3.7 ng/ml) (pooled SEM = 0.7), but GH decreased (P < 0.001) after feeding in both groups. Iv injection of GHRH stimulated release of GH 1 hr before, but not when injected 1 hr after feeding (P < 0.001). In addition, net areas under the GH curve were not significantly different between control and alloxan-treated groups. We conclude that increased concentrations of insulin after feeding do not mediate feeding-induced suppression of GH secretion in steers.     

野生蒲公英联合高山红景天对糖尿病模型小鼠的降血糖作用研究

[目的]探讨长白山野生蒲公英（Taraxacum mongolicum）联合高山红景天（Rhodiola sachalinensis）对糖尿病小鼠的降血糖影响。[方法]选取30只昆明种小鼠,其中24只造成糖尿病模型,分为5组,分别为正常对照组、四氧嘧啶模型组、高山红景天组、长白山野生蒲公英组和联合剂量组。采用自制的水煎液进行灌胃给药,1次/d,连续5 d,测定糖尿病模型小鼠的血糖值。[结果]给药5 d后,各试验组小鼠血糖浓度均明显降低,与四氧嘧啶模型组相比存在极显著差异（P〈0.01）。[结论]高山红景天、长白山野生蒲公英、高山红景天与蒲公英联合对糖尿病小鼠都有明显的降血糖作用。     

Hypoglycemic activity of Nymphaea stellata leaves ethanolic extract in alloxan induced diabetic rats

The ethanolic extract of leaves of Nymphaea stellata given by oral route to diabetic rats at dose of 100 and 200 mg/kg/day for seven days reduced significantly by 31.6 and 42.6 % the plasma glucose level increased by intraperitoneal injection of 120 mg/day of alloxan. Moreover, the treatment significantly affected the plasma level of cholesterol and triglyceride.     

Construction of a New Zealand rabbit model of diabetic nephropathy

AIM: To establish a method to produce animal model of diabetic nephropathy (DN) in New Zealand rabbits using alloxan (ALX). METHODS: Twenty male New Zealand rabbits were randomly divided into 2 groups: 8 rabbits in control group were fed with conventional feed such as buffer solution;12 in diabetes milleuts (DM) group were fed with high-sugar and high-fat diet (conventional feed plus 5% sucrose, 5% pork fat and 1% cholesterol) for 2 weeks, then intravenous injection of ALX at 50 mg/kg was given, and another dosage of 100 mg/kg ALX was intravenously injected after 48 h. The levels of blood glucose, blood cholesterol, triglyceride, urine microalbumin, and serum creatinine were detected before and 12 weeks after the processes of modeling. After 12 weeks, the rabbits were sacrificed,and the kidneys were taken for pathological examination. RESULTS: The successful model of DN in the rabbits had the characteristics of high blood glucose, abundant urine microalbumin (P<0.01), and corresponding morphological and functional changes of the kidneys. CONCLUSION: Rabbits with high-sugar and high-fat diet plus twice intravenous injection of ALX at a total dose of 150 mg/kg (50 mg/kg and 100 mg/kg, respectively) shows low mortality and stable diabetes mellitus state. This method induces a typical DN model in 12 weeks.