调环酸钙25%悬浮剂对直播稻抗倒伏和产量性状的影响

为了研究新型植物生长调节剂调环酸钙在直播稻上的应用效果,本研究设计了调环酸钙25%悬浮剂在直播稻武运粳8号上的田间试验,用来了解其对直播稻的抗倒伏性状和产量性状的影响。试验结果表明:在直播稻武运粳8号的分蘖期使用调环酸钙25%悬浮剂12~15g/667m2均能够显著降低水稻的株高,缩短茎基部节间长度,提高水稻的抗倒伏能力,同时也能显著提高水稻产量。     

不同剂量25羟基维生素D3对断奶前犊牛血液生化指标、抗氧化能力和免疫功能的影响

为研究不同剂量25羟基维生素D₃[25(OH)VD₃]对断奶前犊牛血液水平、抗氧化能力和免疫功能的影响,选取18 头健康状况良好的新生荷斯坦犊牛,随机分成3 组,每组6 头进行为期56 天的饲养试验。分为对照组[不添加25(OH)VD₃]、低剂量组[25(OH)VD₃添加量6 000 IU/头·天]和高剂量组[25(OH)VD₃添加量12 000 IU/头·天]。结果表明,与对照组相比,随着25(OH)VD₃添加剂量增加,血浆生长激素、胰岛素样生长因子-1、过氧化氢酶、超氧化物歧化酶、总抗氧化能力以及免疫球蛋白G(IgG)含量显著增加(P<0.05),而丙二醛含量随剂量增加而存在降低趋势(P=0.079)。与对照组和低剂量组相比,高剂量组血浆免疫球蛋白A(IgA)含量有增加趋势(P=0.059)。综上所述,断奶前犊牛饲喂高剂量25(OH)VD₃有利于促进体内生长激素和胰岛素样生长因子-1分泌,可提高犊牛抗氧化能力,增强免疫功能。     

Inlfuence of FSH Treatment on Expression of CDC25A,TSSK3 and P53in Vitro Cultured Sertoli Cells of Calf

CDC25A, TSSK3 and P53 expressionsin vitro in cultured sertoli cells after FSH treatment were studied in order to provide some data for further researches of spermatogenesis. Different concentrations of...     

小麦矮腥黑粉菌g9890基因编码效应蛋白的生物信息学分析及亚细胞定位

为明确小麦矮腥黑粉菌Tilletia controversa g9890基因编码效应蛋白的生物学功能,根据小麦矮腥黑粉病菌转录组测序结果,筛选出效应蛋白g9890,通过PCR技术获得g9890基因cDNA的全长序列,并对其进行生物信息学以及亚细胞定位分析。多种生物信息学数据库分析表明,g9890基因全长为1 038 bp（包括终止密码子）,共编码345个氨基酸,相对分子质量为37 353.32,理论等电点为5.02。g9890效应蛋白不稳定系数为15.94,疏水性指数为-0.312,是一种亲水性且稳定的蛋白。将g9890基因与pbin-GFP载体重组,利用冻融法转化至根癌农杆菌Agrobacterium tumefaciens GV3101中,将其注入烟草进行瞬时表达分析,并通过共聚焦激光显微镜观测该基因的定位状况,结果显示,g9890定位在细胞膜和细胞核上。     

Work-hardening Mechanism of Non-magnetic High Manganese ZG25Mn18Cr4 Castings

The important character of non-magnetic high manganese ZG25 Mn18Cr4 alloy is rapid work-hardening under external stress. With X-ray diffraction and TEM analysis, it is proved that the microstructures of both undeformed and deformed matrixes are austenite and carbide with some striation structure in the matrixes, the amount of stripes in the deformed matrix is obviously more than that in the undeformed matrix. Electronic diffraction analysis confirmed that the stripes in coarse austenite grains are high density lamination fault sheets. A great deal of lamination fault sheets produced by plastic deformation divides up austenite matrix, shortens the free distance of dislocation movement and results in the rapid work-hardening of ZG25Mn18Cr4 alloy.     

滩羊毛色候选基因MC1R和TCF25的mRNA表达量定量分析

为了探究影响哺乳动物毛色的黑色素皮质受体-1基因(MC1R)和转录因子25基因(TCF25)与滩羊毛色的关系,本研究利用实时荧光定量PCR(qRT-PCR)技术,对15只不同毛色滩羊(纯白、褐斑、黑斑)皮肤组织中MC1R基因和TCF25基因的mRNA表达量进行研究。结果显示,MC1R基因和TCF25基因在3种不同毛色滩羊中均有表达。MC1R基因在不同毛色滩羊皮肤组织中表达量为黑斑>褐斑>纯白,其中黑斑组极显著高于纯白组(P<0.01),显著高于褐斑组(P<0.05),褐斑组显著高于纯白组(P<0.05);TCF25基因在不同毛色滩羊皮肤组织中表达量为纯白>褐斑>黑斑,组间差异不显著(P>0.05)。试验表明了目的基因表达量与滩羊毛色间的关系,为今后滩羊毛色选育与品种改良提供参考。     

鲤trim25多拷贝基因进化和表达调控初探

天然免疫系统是硬骨鱼类抵抗病毒感染的主要防御系统,三重基序(tripartite motif, TRIM)蛋白家族作为天然免疫系统的重要组成部分,参与病毒感染的免疫网络调控,其中,TRIM25已被证实在多种鱼类的免疫反应中发挥重要作用。本研究对鲤(Cyprinus carpio) trim25基因的16个拷贝进行了序列进化分析、共线性分析和功能域结构分析,并比较了各拷贝在组织中的表达和顺式调控位点的差异。序列比对和系统进化分析结果均显示,位于鲤11和12号染色上、结构完整的TRIM25的2个拷贝与金线鲃(Sinocyclocheilus grahami)和斑马鱼(Danio rerio)的TRIM25蛋白结构高度相似,与鲤科鱼类以外的其他物种的结构差异较大。基因共线性结果显示,trim25基因上下游基因在不同物种的进化过程中相对保守。鲤TRIM25蛋白的结构分析显示,在鲤TRIM25的16个拷贝中,有6个拷贝具有完整功能结构域,其中,各有5个拷贝在鲤的肝与脑组织中高表达。在构建的表达数量性状基因座(eQTL)调控网络中,在肝和脑组织中分别筛选到5个和17个顺式调控trim25基因表达的单核苷酸多态性(SNP)位点。本研究对鲤trim25基因多个拷贝的序列差异进行了比较,并对鲤与其他物种TRIM25的序列、进化关系和共线性相似度进行了比较,揭示了鲤trim25各拷贝间的结构多样性和在组织中的表达情况,筛选出了可能调控trim25基因表达的SNP位点,为今后研究鲤TRIM25相关的调控和抗病研究提供了理论依据。     

Determination of primary structure of amberjack myosin heavy chain and its relationship with structural stability of various fish myosin rods

The structural stability of fish myosin depends upon species and temperatures of water in which fish live. Primary, secondary, and quaternary structures of myosin heavy chain (MyHC) from three species of fish living at different temperature ranges have been compared with those of rabbit MyHC in order to investigate the differences in stability. Primary structure of MyHC, although being accessible for warm-water and cold-water fish (carp and walleye pollack), was not available in previous for tropical-water fish literature; so in this study primary structure of MyHC of the tropical-water fish amberjack has been determined by cloning and sequencing its cDNA. The MyHC has 1938 amino acid residues (AA), which are almost as much as as those of carp and walleye pollack. The amberjack MyHC is 91–95% homologous with other fish and rabbit MyHCs. There is a discernible difference between animal species with stable myosin rod (amberjack, carp, and rabbit) and walleye pollack with unstable rod. Stable rod species have a high probability of forming coiled-coil around the COOH-terminal end of the rod, while the pollack has a low coiled-coil formation probability. In addition, the average scores of the coiled-coil for myosin rod were rabbit (1.738) > amberjack (1.691) > carp (1.680) > walleye pollack (1.674) which correlated exactly with the observed stability. The results suggest that coiled-coil forming ability, particularly around the COOH-terminal end, directs structural stability of fish myosin rod.