蟾蜍自然脱衣及加工方法

蟾蜍（癞哈蟆）脱衣有边蜕皮边吃，蜕完吃光的怪习，所以蟾衣极难找到．开展人工养蟾蜍，可获取珍世稀药．但不同的采集蟾衣技术，其效果也不同。蟾蜍自然脱衣，药用价值高，也不伤害蟾蜍，能获得较好的经济效益。 １、蟾蜍品种的选择 用来蜕衣的蟾蜍应体大、肉肥、健壮、无损伤，这是高产的基础。因此，宜选择中华蟾蜍及亚种和头盔蟾蜍、黑眶蟾蜍、喜山蟾蜍等。这类蟾蜍种群大、分布地域广。其次，要充分利用好蜕衣高产质优的年龄段，以２～３年龄的成年蟾蜍最好，此时生长旺盛。幼蟾蜍蜕衣会影响生长发育，老蟾蜕衣质量差，产量低，均不宜…     

药物刺激影响质量 蟾衣价格大幅回落

近一年来,社会上有关对蟾蜍蜕衣技术炒作十分火热.据笔者调查了解,其中有真有假也有诈,人们要尽快识破蟾蜍蜕衣宣传中的误导,走出误区,不再上当受骗.     

蟾蜍壳采集器

蟾蜍壳指的是蟾衣，系中华大蟾蜍(俗称癞蛤蟆)、黑眶蟾蜍等蟾蜍科两栖动物生理性蜕下的角质衣膜，是用于肿瘤及疑难杂症的传统中药材。     

第五代采集蟾衣技术优点多

浙江省海宁市龙头阁两栖爬行动物研究所发明的集约高产免守候采纯天然蟾衣的国家专利技术及配套技术优点多。此技术胜过前四代技术，第五代技术全免守候，不影响人白天上班和晚上睡觉，蟾蜍不吃衣，不必用药物，让活蟾蜍连续脱衣，整衣率可大大提高，一个人一天可管2000只蟾蜍脱衣。     

纯天然蟾衣采集技术

蟾蜍（俗称癞蛤蟆）角质层所脱下的表皮是蟾衣,是名贵的中药材。夏秋季是野生蟾蜍活动的旺季,在各地菜园、路旁、田间均有分布。     

专利技术采蟾衣并收衣供龟种

新型蟾蜍壳采集器专利号:ZL200620104403·1蟾蜍壳采集器专利号:ZL01210442·6天然蟾衣整理具专利号:ZL01246747·2绿毛龟光帘培育法专利号:ZL00115741·8捕蛇网专利号:ZL00249846·4我所高级农技师蒋张林养蟾蜍18年,现发明的专利新型蟾蜍壳采集器整套连贯的第六代技术可使蟾蜍连续蜕蟾衣,一只蟾蜍一年可蜕10～40张天然衣,整衣率最高达98%,自动防逃,并免修补,且不用药刺激,不用人工守候,     

Effects of Donor Cells Treated with Egg Extracts on the Development Competence of Cloned Embryos

Japanese Balck cattle fetal fibroblasts (JBCFF) were induced with Xenopus leavis egg extracts and somatic cell nuclear transfer (SCNT) was carried out with the reprogrammed JBCFF as donor cells in order to investigate their effects on SCNT efficiency.Three samples of egg extracts were acquired from different Xenopus laevis.The protein contents and kinds in extracts were evaluated with BCA Protein Quantification Kit and SDS-PAGE.Concentration of Digitonin to permeabilize JBCFF was optimized and assessed with PI staining.Reprogrammed cells treated with egg extract were used as donor in SCNT.Additionally the reconstructed embryos were activated with ionomycin+6-DMAP and A23187+6-DMAP to compare their effects on the development competence.The protein contents of extracts samples were 56.2255,64.6570 and 71.2158 μg/mL,respectively,the each extract had the same composition about 40-55 and 70-100 ku.The optimal concentration of Digitonin was 7 μg/mL and the permeabilization rate was 55.44%.After extracts treatment and continuous culture for 6-7 d,JBCFF formed well-defined colony structures.No significant composition difference was found in rates of fusion (92.83% vs 96.04%),cleavage (89.64% vs 89.78%) and blastocyst formation (24.06% vs 23.12%) of cloned embryos when the colony cells and JBCFF without extracts treatment were used as donor cells (P>0.05).Similarly,the two activation methods had no significant effect on the developmental competence of cloned embryos (cleavage rate 92.16% vs 92.28%,blastocyst rate 23.21% vs 24.18%).Conclusively,Xenopus leavis,egg extracts could induce JBCFF reprogramming to a low differentiated state.However donor cells with reprogramming partially could not improve the development of cloned embryos and its mechanism requires further research.     

不同霉变程度蟾皮气味检测及表面真菌研究初探

为确定电子鼻技术能否区分不同霉变程度蟾皮,探索蟾皮表面真菌种类,试验收集当季(秋季)新产蟾皮,在高温高湿环境下培养15 d,每3 d取样1次进行电子鼻检测及菌落计数,采用主成分分析、载荷分析、线性判别分析对电子鼻响应值进行处理。同时,采用平板法培养、纯化蟾皮表面真菌,获得单一菌株,结合菌落形态、显微结构、分子鉴定三种方法对真菌进行鉴定。结果表明：主成分分析可有效区分新产(为0天)蟾皮与3,6,9,12,15天蟾皮,但对6,12,15天蟾皮区分度差;蟾皮气味变化主要表现在甲烷等短链烷烃(W1S)、长链烷烃(W3S)、醇醚醛酮类(W2S)和氮氧化合物(W5S)四根传感器上;与主成分分析相比,线性判别分析区分效果更好。从蟾皮表面共分离到6种真菌,可能为木贼镰刀菌(Fusarium equiseti)、草酸青霉(Penicillium oxalicum)、赭曲霉(Aspergillus ochraceus)、黄曲霉(Aspergillus flavus)、绳状篮状菌(Talaromyces verruculosus)和亮白曲霉(Aspergillis candidus)。说明利用电子鼻技术检测...     

专利技术采蟾衣并收衣供龟种

我所高级农技师蒋张林养蟾蜍18年，现发明的专利“新型蟾蜍壳采集器”整套连贯的第六代技术可使蟾蜍连续蜕蟾衣，一只蟾蜍一年可蜕10-40张天然衣，整衣率最高达98％，自动防逃，并免修补，且不用药刺激，不用人工守候，     

专利技术采蟾衣并收衣供龟种

一种带分离机构的蟾衣采集器专利号:ZL200620140081·6新型蟾蜍壳采集器专利号:ZL200620104403·1蟾蜍壳采集器专利号:ZL01210442·6天然蟾衣整理具专利号:ZL01246747·2绿毛龟光帘培育法专利号:ZL00115741·8捕蛇网专利号:ZL00249846·4