积极发展订单农业努力做大香猪产业

从江香猪是我国稀有的优良地方猪种，它以其“体型矮小、肉质香嫩、基因纯合、纯净无污染”等独特优点而享誉国内外。自１９８０年以来，全国各地许多科研单位和高等院校对从江香猪进行了深入广泛的研究，一致认为：从江香猪的肉质品位高，营养价值丰富，１８种必需氨基酸含量齐全，特别是有利人体脑神经健康的谷氨酸含量较高，是有益于人们健康的时尚食品。同时，从江香猪的生理指标与人体相似。因此，从江香猪不仅是制作高档肉食制品的上乘原料，而且还是医学实验动物的理想猪种。１９８２年，从江香猪已被编入《贵州省地方畜禽品种志》；…     

Cloning of Inhibin-α Gene and its mRNA Expression Pattern in the Ovary of Congjiang Xiang Pig

To reveal the relationship between inhibin-α(INHA) gene and the reproductive traits of Congjiang Xiang pig, INHA gene was cloned and sequenced taking the genomic DNA of Congjiang Xiang pigs as templates by polymerase chain reaction(PCR) method.The polymorphisms of INHA gene were tested in Congjiang Xiang pig populations with high-litter size and low-litter size using allele-specific PCR(AS-PCR) method.The expression profile of INHA gene in ovaries was detected from Congjiang Xiang pigs with high-litter yiled or low-litter yiled by Real-time PCR method.The complete coding region of INHA gene was 1095 bp in length, which coded for 364 amino acid residues.Compared with the known sequence, two candidate sites, G359A and A373G, were found out from exon 2 region of INHA gene in Congjiang Xiang pig.After investigation for the two sites in a large population, the frequency of alleles between two populations was not significant and without obvious relativity with the litter yiled of Congjiang Xiang pig.However, the INHA mRNA level in the ovary of Congjiang Xiang pig with high-litter yiled was higher than that with low-litter yiled.It suggested that INHA gene was much conserved, INHA gene expression level might be concerned for the regulation of ovary growth and follicle development in Congjiang Xiang pig breed.     

从江县柑桔黄化的原因及综合防治措施

被国家林业部授予“中国名特优经济林椪柑之乡”荣誉称号的贵州省从江县,柑桔种植面积达0.55万hm2,柑桔已成为从江县的支柱产业,近年来,全县柑桔大面积发生黄化病,已经严重影响柑桔的产量和品质,成为广大果农十分关注的热点和难点问题,严重威胁从江县柑桔产业的可持续发展,引起     

火焰原子吸收光谱法测定从江椪柑中的微量锌

通过正交试验优化组合,得到最佳仪器工作条件,用火焰原子吸收光谱法测定微量元素锌。结果表明:线性范围为0￣2.000μg/ml,标准曲线相关系数r为0.999 89,特征浓度为0.019(μg/ml)/1%,检出限为0.05μg/ml,标准偏差SD为0.38%,相对标准偏差RSD为1.43%,回收率为99.3%。该方法简便,灵敏度高,结果准确。     

贵州3个地方猪种的mtDNA D-loop序列遗传多样性分析

为了研究3个贵州地方猪种(白洗猪、从江香猪和黔北黑猪)mt DNA D-loop区的遗传多样性,本试验采用PCR直接测序的方法测定了3个贵州地方猪种共57个个体的mt DNA D-loop区全序列。结果表明,3个贵州地方猪种mt DNA D-loop区序列长度为1254~1314bp,可分为高变区、串联重复区和保守区,串联重复片段有4种,可分为A型和B型;D-loop区的碱基含量特点为A+T明显高于G+C;在白洗猪、从江香猪和黔北黑猪的高变区分别检测到6、6、8个均为转换的突变位点,分别定义了4、4、6种单倍型,单倍型多样度(Hd)为0.6386~0.8974,核苷酸多样度为0.0025~0.0035,3个贵州地方猪种均表现出较为丰富的遗传多样性。     

从江椪柑常温保鲜贮藏试验

(木)柑,又称卢柑,为芸香科柑桔属.1985年贵州省从江县从湖南省湘西自治州引进,经过近20年的努力,产量逐渐上升,由于大多数果农缺乏就地贮藏保鲜技术,造成果实的积压和品质下降,价格低廉,因而果农实际收入不大.为解决(木)柑生产中的贮藏保鲜问题,我们利用5种化学保鲜剂在黔东南民族职业技术学院进行了常温保鲜处理试验.     

从江香猪、环江香猪内脏器官测定初报

香猪是我国著名的小型地方猪种，国家一级保护畜种。具有体型矮小、肉质优良、基因纯合、纯净无污染，抗逆性强等特点。香猪产于黔桂两省交界的九万大山之中，受地理条件限制，饲粮不足，猪自繁自养，长期高度近交繁殖形成了具有上述特点的小型猪。     

沼肥在从江椪柑上的施用效果

沼肥是有机物经过厌氧发酵处理后产生的无公害有机肥，它的养分能被植物直接吸收利用，沼肥用于种植业，不仅能提高果品产量，而且能提高果品品质。从江县是贵州省重点优质椪柑生产区，全县有果林0．54余万hm^2，是当地农户赖以增收的支柱产业。为了探索沼肥对从江椪柑的生长、产量、品质及改良土壤等方面的作用，贵州省农村能源办、黔东南州农村能源办专门安排项目和经费支持开展这项工作，并于2006年在翠里乡高文村进行了沼肥在从江椪柑上的施用试验。