排序方式: 共有41条查询结果,搜索用时 15 毫秒
1.
2.
<正>1能量与蛋白蛋白质、能量营养不良可导致胸腺萎缩,使迟发型皮肤超敏反应降低;T细胞特别是辅助性T细胞数量减少、淋巴细胞对丝裂原的反应性降低、胸腺素活性降低,分泌型免疫球蛋白IgA抗体反应功能下降,抗体亲合力降低,补体浓度和活力降低,吞噬细胞功能异常,尤其是必需氨基酸缺乏对于非特异性免疫和特异性免疫都具有明显的抑制作用。 相似文献
4.
Production and Identification of High Affinity Monoclonal Antibodies Against Pesticide Carbofuran 总被引:2,自引:0,他引:2
YANG Jin-yi WU Qing WANG Hong PAN Ke LEI Hong-tao HU Da-yin SHEN Yu-dong XIAO Zhi-li ZHENG Xiao-feng SUN Yuan-ming 《中国农业科学(英文版)》2007,6(9):1082-1088
To produce high-affinity monoclonal antibodies against pesticide carbofuran, and the develop immunochemical assays for people's health and environmental protection, the hapten 4-[[(2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy) carbonyl]- amino]-butanoic acid (BFNB) of carbofuran was synthesized and Balb/c mice were immunized by the hapten-carrier (BFNB-bovine serum albumin, BFNB-BSA) conjugates. The splenocytes of immunized mice were fused with Sp2/0 cells and the cultural supernatants of hybridoma cells were screened by the indirect enzyme-linked immunoabsorbent assay (ELISA), based on BFNB-ovoalbumin conjugates (BFNB-OVA). Purified monoclonal antibody (McAb) was obtained from fluids of ascites, deposited by octanoic acid and ammonium sulfate. The affinity and the specificity of McAb were characterized by ELISA or indirect competitive ELISA. A hybridoma cell line (5D3) secreting anti-carbofuran McAb had been established. The titer of culture medium and ascites was up to 1:2.048 × 10^3 and 1:1.024 × 10^6, respectively, and the subtype of the McAb was IgG1. The affinity constant of the McAb was about 2.54 × 10×9 L mol^-1, with an IC50 value of 1.18 ng mL^-1 and a detection limit of 0.01 ng mL^-1. Cross-reactivity studies showed that the McAb was quiet specific for carbofuran, as among the four analogous compounds, they were all hardly recognized (4.59 × 10^-4% for 2,3-dihydro-2,2- dimethyl-7-benzofuranol and less than 3.0 × 10^4% for others). The prepared McAb had a very high affinity and specificity, and it could be used to develop ELISA for rapid determination of carbofuran. 相似文献
5.
我所2003年引进美国杏李恐龙蛋,将其接穗高接在3年生凯特杏上,第2年每株结果2-3千克,今年是第3年.每株结果20千克左右,而且果实特大,平均果重125克,最大果200克以上。实践证明,凯特杏改美国杏李恐龙蛋嫁接亲合力强,生长迅速,果实发育快,果实特大,丰产稳产,其经济效益是凯特杏的数倍,建议在生产中大力推广。具体操作如下。 相似文献
6.
7.
8.
梅花粉生活力和授粉亲合力的测定 总被引:3,自引:0,他引:3
张彦书 《四川农业大学学报》1990,8(1):27-31
试验结果:①梅的花对低温的忍耐力较强,花粉发芽所要求的温度较低,适应范围较广;低浓度的蔗糖琼脂培养基对花粉发育有利,高浓度的培养基对梅的花粉发芽有抑制作用。②梅是自花能结实的果树,但异花授粉更能增产。 相似文献
9.
该文讨论了土壤对磷的吸附/解吸特性与磷矿粉供磷关系。结果表明,土壤磷的吸附数据符合Langmuir和Fruendlich等温吸附方程,磷的最大吸附量(mg/kg)为郴州红壤(1167)〉黄棕壤(1111)〉棕红壤(651.7);磷的亲和力b值(L/mg)为郴州红壤(0.38)〉棕红壤(0.09)〉黄棕壤(0.04);磷的最大缓冲容量(L/kg)为郴州红壤(441)〉棕红壤(58.4)〉黄棕壤(44.2);磷吸附的相对容量Kf值(L/kg)为郴州红壤(434.9)〉黄棕壤(226.7)〉棕红壤(195.0);比较相关系数和标准误差,Freundlich方程更适合拟合磷的吸附数据。郴州红壤对磷的亲和力较大,吸附磷量也大,但磷的解吸量较小,供磷能力弱,土壤中有效磷含量也低;黄棕壤和棕红壤对磷的亲合力弱,吸附磷量少,但磷的解吸量较大,供磷能力强,土壤中有效磷含量也较高。不同的磷矿粉供磷能力也与2%枸溶性磷含量有良好的线性关系。 相似文献
10.
用电导频散法研究Cl-、SO42-和H2PO4-阴离子与土壤的相互作用 总被引:2,自引:0,他引:2
本文介绍了采用电导频散装置测量分别含有10-4mol/LHCl、5×10-5mol/L H2SO4和10-4mol/L H3PO4的黄棕壤、棕壤、黑土和砖红壤悬液(20-30g/kg)的电导频散曲线,频散曲线上均呈现频率范围相当宽的坪区,频散曲线的特性分析结果表明,黄棕壤和棕壤在3种酸溶液中的始散频率(0.6-1kHz)低于黑土悬液(16-28kHz),而砖红壤的始散频率最高(25-47kHz;Cl-、SO42-和H2PO4-离子与4种土壤的相对亲合力(REC1.5/RECtp-1)顺序为Cl- < SO42- < H2PO4-,在不同土壤之间的顺序则随酸溶液而异. 相似文献