基于遥感指数的草原火后植被恢复研究

草原火是草原生态系统重要的干扰因子，严重影响着系统的结构与功能。基于遥感数据，以2015年“4·16”特大草原火灾为例，利用ENVI和ArcGIS等软件，分别对NDVI和GPP指数及火烧严重度下的植被恢复过程进行定量分析。结果表明，基于NDVI和GPP指数的火后植被恢复过程表现相似，不同年份植被恢复情况存在一定差异。火灾发生当年（2015年）火烧迹地植被恢复状况高于未发生火灾区域，而在火后第1年（2016年）却又稍低于未发生火灾区域，直到火后第2年（2017年），火烧迹地植被基本恢复到火前状态。同时，不同火烧严重度下的植被恢复过程在存在明显差异。草原火灾发生后当年（2015年），中强度火烧下植被恢复最好，其次是轻度，重度表现最差。草原火烧严重度对植被恢复的影响，主要表现在火后第1个植被生长季。在之后的年份里，由于草原更新能力强大，不同火烧严重度对草原植被的影响不明显。本研究可丰富草原火灾及火烧迹地植被恢复等相关研究成果，同时为草原火灾管理和草原生态系统的可持续发展提供科学依据。     

In situ radiometric mapping of soil erosion and field-moist bulk density on cultivated fields

Abstract. Recent developments in in situγ ray spectrometry offer a new approach to measuring the activity of radionuclides such as ¹³⁷Cs and ⁴⁰K in soils, and thus estimating erosion or deposition rates and field moist bulk density (ρ_m). Such estimates would be rapid and involve minimal site disturbance, especially important where archaeological remains are present. This paper presents the results of a pilot investigation of an eroded field in Scotland in which a portable hyper pure germanium (HPGe) detector was used to measure γ ray spectra in situ. The gamma (γ) photon flux observed at the soil surface is a function of the ¹³⁷Cs inventory, its depth distribution characteristics and ρ_m. A coefficient, Q_Cs, derived from the forward scattering of ¹³⁷Cs γ ray photons within the soil profile relative to the ¹³⁷Cs full energy peak (662 keV), was used to correct the in situ calibration for changes in the ¹³⁷Cs vertical distribution in the ploughed field, a function of tillage, soil accumulation and ρ_m. Based on only 8 measurements, the agreement between in situγ ray spectrometry and soil sample measurements of ¹³⁷Cs inventories improved from a non significant r²=0.05 to a significant r²=0.62 (P<0.05). Erosion and deposition rates calculated from the corrected in situ¹³⁷Cs measurements had a similarly good agreement with those calculated from soil cores. Mean soil bulk density was also calculated using a separate coefficient, Q_K, derived from the forward scattering γ photons from ⁴⁰K within the soil relative to the ⁴⁰K full energy peak (1460 keV). Again there was good agreement with soil core measurements (r²=0.64; P<0.05). The precision of the in situ¹³⁷Cs measurement was limited by the precision with which Q_Cs can be estimated, a function of the low ¹³⁷Cs deposition levels associated with the weapons testing fallout and relatively low detector efficiency (35%). In contrast, the precision of the in situ ρ_m determination was only limited by the spatial variability associated with soil sampling.     

Genetic Engineering Approaches to Pig Production*

Modern biotechnology promises a number of new applications in animal breeding and production. Although conventional pig breeding has achieved a high level of efficiency and productivity numerous problems have been encountered with animal health and the loss of meat quality. Selection based on phenotypic performance data of individual animals does not take into account the importance of specific genes and their relevance within a complex regulatory system. In most cases it is therefore difficult to trace back the genetic origins of clinically important disorders. The application of genetic engineering techniques in pig production will facilitate diagnosis, improvement of productivity, and animal health by allowing direct genetic manipulation. Attention must be focussed on the physical and genetic analysis of the procine genome. The isolation and characterisation of genes, DNA-markers, polymorphic DNA-fragments, and their chromosomal assignment will be important prerequisites and tools for the elucidation of genetic disorders. Especially the detection of heterozygous carriers of recessive disorders and their elimination from the breeding stock will increase selection accuracy and decrease the generation intervals. But also the rapid and simple detection of infectious diseases, which is sometimes difficult if not impossible at present, will improve animal health and welfare. Although the production of transgenic animals either by DNA-microinjection into zygotes or the use of embryonal stem cells manipulated in vitro is less straightforward than DNA-based diagnosis it will play an important role in the direct manipulation of the porcine genome and genes. Breeding programmes including the use of transgenic livestock have already been developed. There is no doubt that genetic engineering has reached a degree of practical feasibility, allowing it to play an important role in pig breeding in particular and animal production in general.     

荒漠化能量与水平衡监测系统的应用

利用卫星遥感技术进行宏观监测是我国荒漠化监测的重要手段,它具有高效经济的特点。本文阐述了中荷合作项目中荒漠化能量与水平衡监测系统的技术方法及主要应用。     

加热光纤法同步测定土壤水热参数误差分析

土壤水热参数是研究土壤水热传输的基本物理参数。当前热脉冲探针法（HPP）可同步测定土壤水热参数,但该方法仅限于在点尺度下测定。与其具有相同理论基础的加热光纤法（SPHP-DTS）,可将测定尺度增大至田间千米尺度,但其测定精度尚未得到有效验证。为了探知SPHP-DTS法的误差,本研究进行了SPHP-DTS法与HPP法测定土壤水热参数的对比试验。结果表明,以HPP为标准,加热光纤法测定热导率的精度RMSE为0.13 W?m-1?℃-1。SPHP-DTS法测定的热导率显著高于HPP法,主要原因在于加热光纤时产生的温度效应。通过热导率法测定土壤含水率时,在热导率测定误差的影响下,SPHP-DTS法的测定精度明显低于HPP法。SPHP-DTS法测定土壤水热参数的其他误差来源包括光纤与土壤之间多个界面的接触热阻、光纤的温度敏感性、噪音干扰以及温度梯度驱动下的水分迁移。本研究可为SPHP-DTS法提升土壤水热参数测定精度提供理论参考。     

An investigation of uncertainty in field habitat mapping and the implications for detecting land cover change

Land cover data for landscape ecological studies are frequently obtained by field survey. In the United Kingdom, temporally separated field surveys have been used to identify the locations and magnitudes of recent changes in land cover. However, such map data contain errors which may seriously hinder the identification of land cover change and the extent and locations of rare landscape features. This paper investigates the extent of the differences between two sets of maps derived from field surveys within the Northumberland National Park in 1991 and 1992. The method used in each survey was the Phase 1 approach of the Nature Conservancy Council of Great Britain. Differences between maps were greatest for the land cover types with the smallest areas. Overall spatial correspondence between maps was found to be only 44.4%. A maximum of 14.4% of the total area surveyed was found to have undergone genuine land cover change. The remaining discrepancies, equivalent to 41.2% of the total survey area, were attributed primarily to differences of land cover interpretation between surveyors (classification error). Differences in boundary locations (positional error) were also noted, but were found to be a relatively minor source of error. The implications for the detection of land cover change and habitat mapping are discussed.     

Dynamics ofRhizoctonia solani (black scurf) in successive potato crops

The position of plants withRhizoctonia solani sclerotia (black scurf) on progeny tubers was mapped for an experimental field at Haren where potatoes were grown continuously and in rotation with other crops for five successive years, and for another field at Borgercompagnie with a 12 frequency of potatoes during three potato crops. Initially, the distribution of plants with black scurf on both fields was rather dense and homogeneous. In the following years the distribution became heterogeneous and patchy. The local decline ofR. solani AG 3 (the common potato pathogen) in Haren was apparently caused by an unknown factor selectively suppressingR. solani AG 3, while simultaneouslyR. solani AG 5 increased in mass. This AG 5 type proved to be an inferior competitor of AG 3 on the potato plant in a laboratory experiment. The specificR. solani antagonistVerticillium biguttatum did not play a role. A similar factor could have reduced the formation of black scurf in the experimental field at Borgercompagnie, whereV. biguttatum was also too infrequent to account for the decline.R. solani AG 5 was not present here and could not indicate the presence of a selective factor against AG 3.     

South-Western blot mapping方法的建立及其应用于锥虫细胞核中DNA结合蛋白的观察

South-Westerm blot mapping是一种结合Western blotting和Southern blotting某些特点的方法.本文介绍用其成功地观察到锥虫核蛋白中DNA结合蛋白的情况,并对一个分子量在40000左右、于较严谨条件下与DNA结合的核蛋白进行了特性鉴定.该蛋白等量地存在于锥虫的前循环期和血液期,对双链DNA有较大的亲和力,并能与酵母菌复制起始片段结合.本文还介绍了锥虫细胞核的提取技术和核蛋白的制备技术.