The Application of GIS in Small Watershed Classification in Loess Plateau

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超氧化物歧化酶

本文就超氧化物歧化酶的种类、分布、结构、活性部位、催化机理、纯化、活力测定、理化特性等方面作了介绍,并叙述了它的研究动向和发展前景。     

山区农业面源污染的防治途径及对策

结合重庆市地形地貌特点和山区农业面源污染发生的因子，提出山区农业面源污染的防治对策。一方面必需结合当地的自然环境条件和经济发展水平提出农业面源污染的防治规划；另一方面必需结合生态农业建设这一主线，使物质、能量在生态系统内良性循环。紧紧抓住产生农业面源污染源的因子——化肥、农药、农田秸杆、畜禽粪便、农村生活废物等，提出科学的防治和减少途径。     

Detection of Colletotrichum coccodes from soil and potato tubers by conventional and quantitative real-time PCR

Colletotrichum coccodes is the causal agent of the potato blemish disease black dot. Two PCR primer sets were designed to sequences of the ribosomal internal transcribed spacer (ITS1 and ITS2) regions for use in a nested PCR. The genus-specific outer primers (Cc1F1/Cc2R1) were designed to regions common to Colletotrichum spp., and the species-specific nested primers (Cc1NF1/Cc2NR1) were designed to sequences unique to C . coccodes . The primer sets amplified single products of 447 bp (Cc1F1/Cc2R1) and 349 bp (Cc1NF1/Cc2NR1) with DNA extracted from 33 European and North American isolates of C. coccodes. The specificity of primers Cc1NF1/Cc2NR1 was confirmed by the absence of amplified product with DNA of other species representing the six phylogenetic groups of the genus Colletotrichum and 46 other eukaryotic and prokaryotic plant pathogenic species. A rapid procedure for the direct extraction of DNA from soil and potato tubers was used to verify the PCR assay for detecting C. coccodes in environmental samples. The limit of sensitivity of PCR for the specific detection of C. coccodes when inoculum was added to soils was 3·0 spores per g, or the equivalent of 0·06 microsclerotia per g soil, the lowest level of inoculum tested. Colletotrichum coccodes was also detected by PCR in naturally infested soil and from both potato peel and peel extract from infected and apparently healthy tubers. Specific primers and a TaqMan fluorogenic probe were designed to perform quantitative real-time (TaqMan) PCR to obtain the same levels of sensitivity for detection of C. coccodes in soil and tubers during a first-round PCR as with conventional nested PCR and gel electrophoresis. This rapid and quantitative PCR diagnostic assay allows an accurate estimation of tuber and soil contamination by C. coccodes .     

旱地(土娄)土长期定位施肥土壤剖面硝态氮分布与累积研究

通过12a大田长期定位试验研究了在自然降水条件、冬小麦—大豆轮作(或休闲)种植制度下,0～400cm土娄土剖面硝态氮分布与积累的特点。结果表明,长期单施氮肥,氮的表观利用率特别低,仅0.51%,氮肥配施钾、磷肥,氮的表观利用率为25%～35%,氮磷钾平衡施肥及配施有机肥,氮的表观利用率达到50%;施N肥方式显著影响土壤硝态氮积累和淋移,旱地土娄土长期单施化学氮肥或氮钾、氮磷、氮磷钾肥使土壤NO-3-N大量积累和淋移;氮磷钾与有机肥的配合施用能有效地缓解土壤对硝态氮的积累,提高氮肥利用率。     

鄂西南民族地区养殖业与旅游业互动发展研究

为了研究鄂西南民族地区养殖业、旅游业的互动发展,文章在文献分析的基础上,结合实地调研掌握的资料和收集的数据,分析了鄂西南少数民族地区养殖业与旅游业互动发展的意义、基础条件和存在的主要障碍。结果表明,鄂西南民族地区具备开展养殖旅游的基础条件,但在发展观念、目标、层次以及人力资源等方面仍存在一些障碍。因此,文章从发展观念转变、发展规划制定、发展层次提升、人才培养和政策支持等方面提出了促进该地区养殖业、旅游业互动发展的对策建议。     

根区滴灌对干旱山地苹果树生长发育和结实的影响

根区滴灌是将雨水促渗管与传统滴灌相结合而组装集成的一项节水灌溉新技术。为了探讨根区滴灌在山地果园的应用效果,为山地果园的栽培管理提供依据,本文以陕北米脂县6 a生山地红富士苹果树为研究对象,以世界公认的最佳苹果优生区洛川县的降雨量和土壤贮水量为灌水依据,分析了不同灌溉方式对苹果树萌芽、坐果、枝叶生长、果实发育和水分利用效率的影响,以及山地果园土壤水分达到优生区标准后与优生区苹果树生长结果的差异。结果表明,采用根区滴灌能有效提高苹果树的萌芽率和坐果率,萌芽率比传统滴灌提高了2.14%~2.76%,坐果率比传统滴灌提高了0.80%~1.42%;能有效促进苹果树新稍和叶片的生长,新稍长度比传统滴灌提高了1.44%~4.94%,百叶鲜质量比传统滴灌提高了2.13%~4.16%;能显著促进苹果树果实的生长,单果重比传统滴灌增大了7.05~14.99 g,优果率比传统滴灌提高了9.4%~14.7%;能显著提高水分利用效率,灌水量比传统滴灌节约了8.09%,水分利用效率比传统滴灌提高了10.62%~15.30%。可见,根区滴灌技术在陕北黄土丘陵区山地苹果生产中具有广阔的应用效果,但是,单纯依靠人为限量补水,山地苹果还不能达到优生区的生产水平。     

Influences of sand cover on erosion processes of loess slopes based on rainfall simulation experiments

Aeolian-fluvial interplay erosion regions are subject to intense soil erosion and are of particular concern in loess areas of northwestern China.Understanding the composition,distribution,and transport processes of eroded sediments in these regions is of considerable scientific significance for controlling soil erosion.In this study,based on laboratory rainfall simulation experiments,we analyzed rainfall-induced erosion processes on sand-covered loess slopes(SS)with different sand cover patterns(including length and thickness)and uncovered loess slopes(LS)to investigate the influences of sand cover on erosion processes of loess slopes in case regions of aeolian-fluvial erosion.The grain-size curves of eroded sediments were fitted using the Weibull function.Compositions of eroded sediments under different sand cover patterns and rainfall intensities were analyzed to explore sediment transport modes of SS.The influences of sand cover amount and pattern on erosion processes of loess slopes were also discussed.The results show that sand cover on loess slopes influences the proportion of loess erosion and that the compositions of eroded sediments vary between SS and LS.Sand cover on loess slopes transforms silt erosion into sand erosion by reducing splash erosion and changing the rainfall-induced erosion processes.The percentage of eroded sand from SS in the early stage of runoff and sediment generation is always higher than that in the late stage.Sand cover on loess slopes aggravates loess erosion,not only by adding sand as additional eroded sediments but also by increasing the amount of eroded loess,compared with the loess slopes without sand cover.The influence of sand cover pattern on runoff yield and the amount of eroded sediments is larger than that of sand cover amount.Furthermore,given the same sand cover pattern,a thicker sand cover could increase sand erosion while a thinner sand cover could aggravate loess erosion.This difference explains the existence of intense erosion on slopes that are thinly covered with sand in regions where aeolian erosion and fluvial erosion interact.     

黄土丘陵区不同管理方式下草地优势种群的生态位

以吴起县杨青川流域草地为研究对象,对封育(Enclosure)、放牧(Grazing)、施肥(Fertilization)、刈割(Clipping)、灭鼠(Deratization)5种管理方式的草地群落进行调查,并运用Levins生态位宽度和Pianka生态位重叠指数对5种管理方式下主要植物种群生态位特征进行分析。结果表明,达乌里胡枝子(Lespedeza davurica)、铁杆蒿(Artemisia sacrorum)、赖草(Leymus secalinus)、猪毛蒿(Artemisia scoparia)和硬质早熟禾(Poa sphondylodes)的种群总生态位宽度较大,封育放牧施肥刈割灭鼠管理模式下,生态位宽度最大的分别是达乌里胡枝子、赖草、紫花地丁(Viola philippica)、达乌里胡枝子和猪毛蒿,生态位宽度值大于4的物种数分别为6、6、3、4和3种。不同管理方式下各种对间的重叠指数平均值由大到小依次封育放牧施肥刈割灭鼠。施肥管理中,种群间生态位宽度与生态位重叠之间存在线性关系,其它管理方式中种群生态位宽度与生态位重叠之间不存在线性关系。生态位分析表明,封育和放牧管理下种群间对资源的需求趋于相同,彼此间通过竞争共存,有利于群落演替,草地生态系统逐渐得到恢复,施肥、刈割、灭鼠管理使群落物种竞争排序发生变化,破坏了草地生态系统原有的竞争机制,导致群落向简单群落演替。从牧草发展角度出发,封育、施肥和刈割有利于优质牧草生长。     

寒冷地区草原有毒有害牧草监测与防治

草原是内蒙古地区草食畜牧养殖产业健康发展的基础,同时也是优质牧草的主要来源。有毒有害牧草生长扩散预示着草场退化,对当地草食畜牧养殖产业健康发展会造成毁灭性打击。有毒有害植物生长发育,不仅会危害牲畜健康且还会严重消耗土壤的水分和养分,妨碍优良牧草繁殖生长,使草场产量和质量显著下降。在长时间草场利用过程中,很多农牧民群众只注重索取,不注重生态环境保护,使草场生产能力和抵抗能力逐渐变差,给有毒有害牧草繁殖生长提供了条件。因此需要加强寒冷地区草原有毒有害牧草的监测,并采取针对性措施进行防控,降低发病率。该文主要探讨内蒙古呼伦贝尔市寒冷地区草原有毒有害牧草的种类,并提出相应的防治措施。