自由曲面前照灯近光配光的防眩目研究

从驾驶员心理学和生理学角度探讨了汽车夜间会车时眩目的机理．由于传统的前照灯配光设计停留在二维的层面上，设计周期长，且配光效果差，很难达到防眩目的要求．本文通过Reflector CAD软件反求出了符合ECE法规配光要求的自由曲面前照灯反射镜的三维模型，该模型可以直接用于数控程序的编制，减小了前照灯的设计成本和设计周期，利用TracePro软件对模型进行近光配光的仿真实验，通过光线追踪的方法避免了实际配光实验时调整反射镜配光所需的反复制造过程，同时又保证了前照灯防眩目的效果。     

Clone identification and genetic relationship among vine cacti from the genera Hylocereus and Selenicereus based on RAPD analysis

Clones of Hylocereus and of Selenicereus species were distinguished from each other by the banding pattern generated by one to nine 10-mer oligonucleotide primers in the random amplified polymorphic DNA (RAPD) reaction. RAPD analysis was also applied to estimate the genetic relationship among five Hylocereus and nine Selenicereus species. A dendrogram was constructed based on a data matrix of 173 polymorphic bands originated by nine primers. Two groups were identified, one consisting of Hylocereus species and the other consisting of Selenicereus species. These results are consistent with the accepted taxonomic classification of the genera studied. The principal coordinate analysis (PCO), i.e. the plot drawn on the basis of the RAPD data, clearly distinguished between three groups, namely, Hylocereus species, S. megalanthus and the rest of the Selenicereus species studied. PCO thus strongly support the notion that the tetraploid S. megalanthus is an exception among the Selenicereus group. The RAPD results support our hypothesis regarding the allopolyploid (rather than autopolyploid) origin of this species.     

规模化蛋鸡场病原菌溯源与生物安全防控研究

细菌病严重威胁蛋鸡健康,以抗生素为主导的细菌病传统防控方式会导致病原菌耐药性严重、鸡蛋药物残留,影响蛋品安全。要保证蛋鸡产蛋期不用抗菌药物而又能生产出"无菌、无抗"的鸡蛋,是国内外技术难题。本团队以规模化蛋鸡场全封闭鸡舍为研究对象,基于PFGE、MLST、全基因组测序等技术,探明了蛋鸡病原菌的种类及基因组特征;对病原菌的耐药性研究表明,蛋鸡病原菌多重耐药严重,依靠药物防控的方式亟待转变;对规模化蛋鸡场细菌溯源研究结果表明,鸡舍生物媒介(鼠、苍蝇等)和非生物媒介(空气、饮水、饲料、鸡粪等)是蛋鸡病原菌的重要传染来源和传播途径;对规模化蛋鸡场生物安全量化评价结果表明,外部和内部生物安全权重高达92.2%。根据溯源和生物安全权重分析结果,提出了在规模化全封闭蛋鸡舍将蛋鸡细菌病从药物防控转变为生物安全防控的新策略;创新了规模化蛋鸡场细菌病防控的系统技术,实现蛋鸡规模养殖整个产蛋期不用抗生素,为生产"无菌、无抗"安全鸡蛋提供技术支撑。     

土地利用变化对土壤水补给来源影响研究

为探究不同植被类型深剖面土壤水补给来源,以黄土区长武塬为研究区,采集两种土地利用方式(农地、18 a苹果园、26 a苹果园)下20 m深土壤剖面,测定土壤水及其稳定性和放射性同位素含量,基于同位素方法对不同土地利用类型下不同深度土壤水来源进行溯源分析。研究表明：(1)苹果园土壤水含量明显低于农地,说明果园耗水强度更大,对深层土壤水影响更为显著。(2)农地、18 a和26 a苹果园下降水偏移量分别为-22‰、-20‰和-6‰,说明降水补给土壤水后受到蒸发作用影响,且农地土壤水比果园受到的蒸发效应更强。(3)该区土壤水补给主要以活塞流为主;对于补给不同深度土壤的可能水源,6 m以下补给水的同位素组成(δ²H=-83.8‰,δ¹⁸O=-12.1‰)较0～6 m (δ²H=-68.8‰,δ¹⁸O=-10.1‰)更为贫化,且6 m以下补给水的氢氧稳定同位素组成与日降水量≥50 mm·d^-1的降水更相似。与农地(δ²H=-70.6‰,δ¹⁸O=-...     

Study on Genetic Fingerprinting Technique of Microbial Communities

The 99% of all microorganisms in naturecannot be isolated by standard culture methods without conspicuous external characters to classify them morphologically. In addition, classification based on physiological or biochemical features are often fuzzy. Molecular techniques are used for uncultured microorganisms to explore the diversity of microbial communities and novel resources without the need of cultivation. DNA dynamics and cloning of PCR products obtained from environmental DNA is now routinely used to identify microbial populations in microbial ecology. For practical reasons the approach is not suited to study the complex dynamics of microbial communities in environment. Genetic fingerprinting techniques are better tools, which give complement for the traditional microbiological methods. The methods are rapid and relatively easy to perform, and they allow the simultaneous analysis of multiple samples, which makes it possible to compare the genetic diversity of microbial communities from different habitats, or to study the behaviour of individual communities over time.     

水泵水轮机流动可视化图像解析系

主要介绍了自行开发的水泵水轮机流动可视化图像的解析原理，并就示踪物的追踪与最佳相关域的确定问题进行了深入的分析，介绍了图像解析的程序设计的思想与功能。试验与计算结果表明本套软件适合于旋转的流体机械的流场图像的解析计算。     

横垄耕作的面源污染截留效应研究

为了解横垄耕作的面源污染截留效应,选取吉林省长春市莫家沟小流域横垄耕作坡耕地为研究区,应用210Pbex核素示踪技术得到研究区土壤侵蚀模数为2 268 t/(km2.a),吸附态氮、磷流失负荷分别为4 889、2 022 kg/a;运用RU-SLE2.0模型,模拟在同等立地条件下顺坡耕作土壤侵蚀模数约为6 200 t/(km2.a)。对比结果表明:横垄耕作较顺坡耕作可减少约63%的土壤流失量及随土壤流失的吸附态氮磷负荷。     

黄土高原小流域泥沙来源的复合指纹识别法分析

流域泥沙来源分析对流域治理及水土保持措施评价有重要意义。通过在陕北选定的汇水区内采集的主沟道沟壁、坡地果园、坡耕地及支沟道土壤样品中17种物质的分析,利用Kruskal-WallisH-test和多元判别分析,筛选出土壤全N,低频磁化率χIf、Cu、137Cs和226Ra组成复合指纹识别因子,利用混合模型,对一次洪水携带的泥沙来自4种源地的贡献比进行了动态分析,结果表明:洪水发生时间段内,主沟道发生了重力侵蚀,对今后定量化研究黄土区的重力侵蚀提供了手段。通过加权平均,总体上,本次洪水携带的总泥沙有33.7%来自主沟道,60.0%来自坡地果园,3.0%来自小块的坡耕地以及3.3%来自支沟道。混合模型拟合优度检测值为0.91,证明在黄土高原可以应用复合指纹识别方法研究洪水泥沙来源,并且能对重力侵蚀产沙进行定量分析。