Identification,characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.)

To identify the possible quarantine viruses in seven common sunflower varieties imported from the United States of America and the Netherlands, we tested total RNAs extracted from the leaf tissues using next-generation sequencing of small RNAs. After analysis of small RNA sequencing data, no any quarantine virus was found, but a double-stranded RNA(dsRNA) molecule showing typical genomic features of endornavirus was detected in two varieties, X3939 and SH1108. Full-length sequence and phylogenetic analysis showed that it is a novel endornavirus, temporarily named as Helianthus annuus alphaendornavirus(HaEV). Its full genome corresponds to a 14 662-bp dsRNA segment, including a 21-nt 5′ untranslated region(UTR), 3' UTR ending with the unique sequence CCCCCCCC and lacking a poly(A) tail. An open reading frame(ORF) that encodes a deduced 4 867 amino acids(aa) polyprotein with three domains: RdRP, Hel and UGT(UDP-glycosyltransferase). HaEV mainly distributed in the cytoplasm but less in the nucleus of leaf cells by fluorescence in situ hybridization(FISH) experiment. This virus has a high seed infection rate in the five varieties, X3907, X3939, A231, SH1108 and SR1320. To our knowledge, this is the first report about the virus of the family Endornaviridae in the common sunflower.     

Spatiotemporal patterns of mountain whitefish (Prosopium williamsoni) in response to a restoration of longitudinal connectivity

To examine the role of longitudinal connectivity on the spatial and temporal dynamics of mountain whitefish (Prosopium williamsoni), we quantified movement and population dynamics following installation of the Landsburg Dam fishway, Cedar River, WA, USA. Mountain whitefish is widely distributed, poorly studied and not the focus of restoration. Before the fishway, mountain whitefish were not observed above the dam. Here, we focus on snorkel counts collected at reach and mesohabitat (e.g. pools) scales over 11 summers on the 20‐km above‐dam segment following restoration. A camera within the ladder provided number, size and movement timing, thereby informing on behaviour and recolonisation. Segment‐scale abundance increased following fish passage reaching an asymptote in 7 years, and mountain whitefish were detected throughout the main stem in 10 years. Annual movement through the ladder increased over time and was positively correlated with instream abundance and discharge, but negatively correlated with water temperature. About 60% of fish movements occurred in spring and early summer, potentially for foraging opportunities. Reach‐scale abundance peaked between 7 and 10 km from the dam; deep, cool (~10.6 to 11.6°C) conditions characterised these reaches. At the mesohabitat scale, mountain whitefish detection increased with depth and velocity after accounting for distance from the dam. Our results show how restoring longitudinal connectivity allowed this nontarget species to colonise newly available habitat. Their response supports the critical roles of longitudinal connectivity and environmental conditions, that manifest at different spatial scales, in dictating how freshwater fish respond to habitat disturbance.     

电超滤浸提土壤养分的研究 Ⅰ.石灰性土壤的电超滤特性

石灰性土壤的电超滤(EUF)特性主要是浸提时因需控制电流而必须大大降低电压。在一定范围内,降压程度与土壤 CaCO_3%有密切关系。控流限额愈低,电压需成比例地下降,但土壤EUF-值减小不多。电流控制在一定值时,离子的解吸总量亦为定值。控流主要控制了各石灰性土壤解吸/溶解份额特大的 Ca+Mg 量近于定值,对于含量小的其他养分离子则并无多大影响,仍可区辨各土壤间的差别。通过冬小麦盆栽试验和有关化学测试法等方面的相关研究,表明采用国际通用的5g 土样和控电流方法测定 EUF-K 和-P 等有效养分,对于石灰性土壤也是可行的,但其EUF-(Ca+Mg)值则毫无植物营养有效性的意义。土壤 EUF 值只是一种指数,并非真正的解吸离子量。石灰性土壤 EUF-K 值因浸提时降压控流而略低,80℃时5min 内解吸的钾不一定是非交换性钾。由于 Ca-P 和 CaCO_3在高温高电压时浸出较多,故石灰性土壤 EUF-P 和-(Ca+Mg)的80℃值/20℃值之比,与酸性土壤或非石灰性土壤相比要大得多。     

Direct and Residual Contributions of Symbiotic Nitrogen Fixation by Legumes to the Yield and Nitrogen Uptake of Maize (Zea mays L.) in the Nigerian Savannah

Field experiments were conducted to determine the direct and residual contributions of legumes to the yield and nitrogen (N) uptake of maize during the wet seasons of 1994 and 1995 at the University Farm, Abubakar Tafawa Balewa University, Bauchi, Nigeria, located in the Northern Guinea savannah of Nigeria. Nodulating soybean, lablab, green gram and black gram contributed to the yield and N uptake of maize either intercropped with the legumes or grown after legumes as a sole crop. Direct transfer of N from the nodulating soybean, lablab, green gram and black gram to the intercropped maize was 24.9–28.1, 23.8–29.2, 19.7–22.1 and 18.4–18.6 kg N ha^–1, respectively. However, the transfer of residual N from these legumes to the succeeding maize crop was 18.4–20.0, 19.5–29.9, 12.0–13.7 and 9.3–10.3 kg N ha^–1, respectively. Four years of continuous lablab cropping resulted in yields and N uptake of the succeeding maize crop grown without fertilizer N that were comparable to the yields and N uptake of the succeeding maize crop supplied with 40–45 kg N ha^–1 and grown after 4 years of continuous sorghum cropping. It may therefore be concluded that nodulating soybean, lablab, green gram and black gram may be either intercropped or grown in rotation with cereals in order to economize the use of fertilizer N for maize production in the Nigerian savannah.     

A releaser pheromone that attracts methyltestosterone- treated immature fish in the urine of ovulated female rainbow trout

SUMMARY: Behavioral experiments concerning a releaser pheromone in the urine of female rainbow trout were performed using immature fish administered orally with 17α-methyltestosterone (MT) during the non-spawning season. The urine was collected by catheter. The frequency of entries of test fish was recorded in each channel scented by test and control solutions in a Y-maze trough. The behavior of both MT-treated and control fish demonstrated that they could not discriminate the differences between distilled and environmental water as control solutions. There was also no difference between MT-treated and control fish when distilled and environmental water were introduced. The MT-treated immature fish were attracted to the channel scented by ovulated female urine. Neither coelomic fluid nor the immature female urine had any effect on the behavioral responses of MT-treated fish, while immature control fish had no preference for the urine of ovulated females. These results suggest in rainbow trout that ovulated female urine contains a releaser pheromone to attract mature males, and that androgens are involved in the sensory mechanisms detecting the releaser pheromone in fish.     

辣椒夏季品比试验报告

为了考察我公司新推广品种及新配组合在高温伏旱状况下的适应性，我们于2003年5月7日在公司试验基地进行了品种对比试验。结果表明新推广品种中有九个品种或组合为极耐热或耐热，一个品种耐热性稍差。新配组合中部分表现优良，有推广前景。     

红鳞蒲桃开花物候特性研究

对红鳞蒲桃种群的花期物候，单株、单个花序、单花的开花动态进行系统的观测研究，详细测量并记录花部构件，同时调查红鳞蒲桃种群的同花期植物。结果表明：红鳞蒲桃种群的花期物候为8月1日～9月15日（2008年），持续时间46d；单株花期从23d到33d不等；单花序的花期情况较为复杂，总体表现为花朵数越多，该花序持续时间越长，但也有个别情况出现，在开花中期花序持续时间长，开花后期花序持续时间最短，开花前期花序持续时间介于两者之间；在整个种群花期内单花平均开花时间约5．2d，种群开花前期单花的持续时间约为6．7d，开花中期约为4．7d，末花期约为4．3d，种群开花前期单花的持续时间显著大于种群中后期的持续时间。红鳞蒲桃种群的同花期植物隶属于16科21属22种。     

真核表达NDV F基因重组质粒的构建及其在CEF细胞中的转染

通过PCR方法自含NDVZF基因的克隆质粒中扩增NDVF基因 ,将其与真核表达载体pcDNA3..1/V5_His_TOPO重组。经核酸内切酶酶切 ,阳性克隆鉴定准确无误 ,核酸序列测定结果与原始基因比较 ,同源性大于 99% ,启始密码和终止密码未出现变异 ,将该重组质粒命名为pcDNANDVZF。将pcDNANDVZF在脂质体作用下转染CEF细胞 ,用间接免疫荧光试验检测 ,结果证明pcDNANDVZF可在CEF细胞中大量表达F蛋白。     

咖啡黑小蠹的发生规律及药剂防治研究

咖啡黑小蠢是近年在兴隆华侨农场严重危害中粒种咖啡的重要害虫。据1989年对4—5年生咖啡树调查,植株受害率100％,枝条受害率29.7—82.5％,虫害枯枝率7.65—17.8％。该虫以雌成虫钻蛀咖啡枝条为害,幼虫和成虫取食蛀道壁上的真菌菌丝。田间种群数量通常在3月上旬开始剧增,3月中下旬为高峰期,7月至10月田间虫口极少,11月以后逐渐有虫口及虫枯枝出现。温度是影响虫口波动的主要因素。2.5％溴氰菊酶、25％杀虫双、40％乐菊酯各兑水1000倍对成虫直接触杀效果显著,死亡率均为100％。48％乐斯本、40％氧化乐果400—800倍液等12种杀虫剂田间喷雾对咖啡枝条蛀洞内的成虫、蛹、幼虫防效均不明显。     

大豆室内耐冷筛选及其在田间的应用研究

本试验把生长箱大豆耐低温萌发筛选与田间早春播研究相结合,验证耐低温发芽的可靠性。在实验室,萌发期表现抗冷的大豆,田间出昔期仍表现抗冷或较抗冷。实验表明,大豆出苗的生物学最低温度平均为7.1℃,所需有效积温136℃。地温的高低直接影响大豆早春出苗的多寡,两者呈密切的正相关。黄、青、黑豆中,以黑豆抵抗早春不良气候条件的能力最强。