狗牙根辐射诱变后代外部性状变异分析

对1997-2002年经过辐射诱变得到的狗牙根诱变后代的6个坪用性状指标的统计分析结果表明,1)不同的辐射诱变剂量处理的后代间坪用性状有显著差异,尤其在节间直径和密度上,6 800 rads处理的后代节间都比9 000 rads处理的后代粗,而密度是前者低于后者,2种剂量处理都可以显著降低草层高度;2)不同辐射诱变代数的后代间存在着差异,原始种源进行多代的辐射诱变,可以使得叶片显著变窄,草层高度显著降低,节间变细变长; 3)同一材料在相同辐射剂量诱变处理后匍匐茎不同节之间也存在显著的差异.     

Simulation of future dissolved oxygen distribution in pond culture based on sliding window-temporal convolutional network and trend surface analysis

Dissolved oxygen (DO) is a key ecological factor to measure the quality of water in the aquaculture. As the pond water body is affected by the breeding environment, the spatial distribution of DO shows a certain law in the entire pond. Therefore, to simulate the distribution of DO in aquaculture waters and grasp the temporal and spatial variation of DO is the key to achieving precise regulation of DO. For this purpose, this paper proposed a method for simulating the temporal and spatial distribution of DO in pond culture based on a sliding window-temporal convolutional network together with trend surface analysis (SW-TCN-TSA). This paper first utilized SW to construct DO data sets with different prediction durations, and then used the improved TCN model to realize one-dimensional time series prediction for DO at single monitoring point. Based on the prediction results of DO, a TSA method was performed on the predicted values of DO at the extreme moments of all discrete monitoring points, so as to realize the simulation of the temporal and spatial distribution of DO in the pond. Experimental results show that the SW-TCN model has better prediction performance for one-dimensional time series prediction of DO. Compared with traditional deep networks, such as CNN, GRU, LSTM, CNN-GRU and CNN-LSTM, the values of evaluation indicators (MSE, MAE and RMSE) have been greatly improved. In the process of trend surface fitting, all fitting R² of DO at different water depths are higher than 0.9, indicating that the TSA can accurately reflect the temporal and spatial distribution of DO. This method can provide a basis for the prediction and early warning of DO in the three-dimensional space of the pond and has high practicability in aquaculture.     

拷贝数变异在畜禽中的研究进展

拷贝数变异（copy number variation,CNV）具有多种形式的变异结构,在品种多样性、生物进化和疾病相关性等研究中起着重要作用,并具有片段长度大、覆盖范围广等特点。随着分子生物学的发展及DNA测序技术的日渐成熟,人们对遗传变异的研究不断向DNA分子水平深入,多态性标记在畜禽育种中已逐渐成为动物育种研究的趋势和主流。由于CNV对基因的调控和表达所造成的影响更为显著,因此,CNV在重要畜禽中的研究越来越多。目前,已检测出大量有关畜禽重要经济性状的基因序列变异,并有许多研究均表明CNV与动物的重要经济特征及疾病的发生有关。笔者主要通过参考国内外相关的研究报道,简述了CNV的相关研究背景、概念、突变机制,归纳总结了CNV对牛、羊、猪、鸡的经济性状、繁殖性状和疾病调控的影响,以期通过对这些重要畜禽的基因组学研究揭示其适应性遗传机理和表型性状差异的遗传基础,开发相应的分子遗传标记,为畜禽的标记辅助选育提供理论基础。     

The research in processing fMRI data using temporal-spatial model based on CCA

In the analysis of cognition of human brain,the fMRI is often used.Corelation is a common method in fMRI data detecting,but its ability should be improved.Based on the Canonical Correlation Analysis(CCA),the temporal correlation and spatial correlation are used together to detect the activated area in the fMRI data.By the application of the method in simulation data and fMRI data,it shows that this method is effective in the detecting.     

Differential and successive effects of residue quality and soil mineral N on water-stable aggregation during crop residue decomposition

Residue quality has been shown to influence soil water-stable aggregation (WSA) during crop residue decomposition, but there is still little information about its interactive effect with soil mineral N availability. The aim of this study was to determine the effect of soil mineral N on WSA during the decomposition of two high-C/N crop residues (wheat straw with C/N = 125.6 and miscanthus straw with C/N = 311.3). The two crop residues were combined with three mineral N addition rates (0, 60, and 120 mg N kg⁻¹ dry soil). Respiration, soil mineral N content, and WSA (expressed as mean-weight diameter, MWD) were measured on several dates during a 56-d incubation. The effect of decomposing crop residues on WSA followed two phases. (i) Between 0 and 7 d, the increase in WSA was related to intrinsic residue quality with higher decomposability of the wheat straw resulting in higher WSA. (ii) Thereafter, and until the end of the experiment, mineral N addition rates had a predominant but negative influence on WSA. In this second phase, the average MWD of residue-treated soils was 0.92, 0.55, and 0.44 mm for the 0, 60 and 120 mg N kg⁻¹ dry soil addition rates, respectively. Mineral N addition which did result in higher crop residue decomposition did not lead to higher WSA. WSA during crop residue decomposition is therefore not simply positively related to the induced microbial activity, and changes in microbial community composition with differential effects on WSA must be involved. The impact of high-C/N crop residues inputs on WSA, initially assumed to be low, could actually be strong and long-lasting in situations with low soil mineral N content.     

Temporal shifts in diversity and quantity of nirS and nirK in a rice paddy field soil

Denitrification is an important part of the nitrogen cycle in the environment, and diverse bacteria, archaea, and fungi are known to have denitrifying ability. Rice paddy field soils have been known to have strong denitrifying activity, but the microbes responsible for denitrification in rice paddy field soils are not well known. Present study analyzed the diversity and quantity of the nitrite reductase genes (nirS and nirK) in a rice paddy field soil, sampled four times in one rice-growing season. Clone library analyses suggested that the denitrifier community composition varied over sampling time. Although many clones were distantly related to the known NirS or NirK, some clones were related to the NirS from Burkholderiales and Rhodocyclales bacteria, and some were related to the NirK from Rhizobiales bacteria. These denitrifiers may play an important role in denitrification in the rice paddy field soil. The quantitative PCR results showed that nirK was more abundant than nirS in all soil samples, but the nirK/nirS ratio decreased after water logging. These results suggest that both diversity and quantity changed over time in the rice paddy field soil, in response to the soil condition.     

石羊河流域冬季冻土深度变化趋势及原因

利用1961~2007年石羊河流域上游、中游、下游当地气象站的冬季最大冻土深度、气温、地温、日照时数、降水量、相对湿度、蒸发、积雪资料,分析了近47 a石羊河流域冬季最大冻土深度的空间分布以及时间变化特征,进而采用相关系数法进一步探讨了冬季最大冻土深度变化的原因。结果表明:在空间分布上,石羊河流域冬季最大冻土深度分布与本地地理位置、土壤类型和海拔高度密切相关。石羊河流域冬季最大冻土深度梯度呈南—北走向,最大值出现在南部古浪,最小值出现在北部民勤。时间变化上,近47 a,石羊河流域以及流域上、中游冬季最大冻土深度呈下降趋势,而下游民勤冬季最大冻土深度则呈上升趋势,石羊河流域冬季最大冻土深度在不同时段内存在明显的6~7 a和9~10 a的周期反映,除下游民勤外在20世纪90年代都发生了突变。相关系数法分析表明,影响石羊河流域冬季最大冻土深度的气象因子是主要是热力因子,热力因子中关联最强的是极端最低地温和气温;水分因子中蒸发和冬季最大冻土深度的关联最明显。     

成都典型区轮作与土壤特征类型对有机碳密度影响力比较研究

研究分析轮作制度与土壤特征类型对农田土壤有机碳密度变异的主导作用程度,对提高农田土壤固碳潜力具有重要意义。基于2010年在新都区和郫县采集的土壤样点数据,利用方差分析和逐步回归分析方法研究分析土壤特征类型(土壤质地、土壤亚类、成土母质)和轮作制度对成都平原典型区农田土壤有机碳密度(SOCD)变异的影响,比较土壤特征类型与轮作制度对土壤有机碳密度变异的影响力。结果显示:土壤特征类型和轮作制度均对SOCD的变异具有显著影响,特别是土壤质地类型,其中黏粒含量与各土层的SOCD之间均存在极显著正相关性(P0.01);与轮作制度影响力比较,土壤特征类型对SOCD变异性影响起主导作用,能够分别解释SOCD在0~40、0~20和20~40 cm土层变异性的35.9%、43.0%和16.8%;而轮作制度对SOCD变异性影响表现在0~40和0~20 cm土层,SOCD逐步回归解释能力在土壤特征类型基础上仅增加2.2%和4.5%,轮作制度影响力不足以改变土壤特征类型对区域土壤有机碳密度变异的影响地位。研究和揭示区域农田土壤有机碳密度变异及其驱动力,更应注重土壤特征的影响力,轮作制度影响力可以被忽略。     

黄土丘陵区须根系作物地土壤分离季节变化研究

采用变坡试验水槽的试验方法,研究了黄土丘陵区典型须根系作物玉米和谷子在生长季土壤分离能力的季节变化及潜在影响因素。结果表明:在作物生长季,须根系作物玉米地和谷子地的土壤分离能力具有明显的季节变化(P0.05),并表现出了相似的季节变化模式;两种作物地土壤分离能力的季节变化主要受到农事活动、土壤硬化、水稳性团聚体和作物根系生长的影响;两种作物地的土壤分离能力可以用土壤粘结力、作物根系密度和水流剪切力很好地拟合(R~20.75,NSE0.74)。