‘哈伯’南天竹组织培养和快速繁殖

为建立‘哈伯’南天竹组织培养和种苗繁育技术体系,以半木质化带芽茎段为外植体材料开展植株再生研究。通过观察对比试验法、L₉(3⁴)正交试验设计完全随机法、极差分析、显著性检验、LSD多重比较,探讨了‘哈伯’南天竹组培的最适培养基配方。试验结果表明：最佳诱导培养基为MS + 6-BA 2.0 mg/L + IBA 0.1 mg/L +蔗糖30 g/L,诱导萌动率71.77%,成活率85.51%;最佳增殖培养基为WPM +6-BA 1.5 mg/L + IBA 0.01 mg/L + 蔗糖30 g/L,增殖系数6.3;最佳生根培养基为1/2 MS+ IBA 0.5 mg/L + NAA 1.0 mg/L + 蔗糖20 g/L + AC 0.2 g/L,生根率97.63%;试管苗移入泥炭土:珍珠岩=3:2(V/V)混合基质中,移栽成活率96.67%。该试验建立了高效稳定的组培快繁技术体系,得到的组培苗后代能够稳定的保持母本优良性状,为工厂化育苗提供了技术支撑。     

Three SNPs within exons of INHA and ACVR2B genes are significantly associated with litter size in Dazu black goats

The aim of this study was to analyse the association between single-nucleotide polymorphisms within INHA and ACVR2B and litter size in Dazu black goats. In total, twenty-two SNPs were genotyped in 190 individuals by SNaPshot and resequencing. The results showed that three SNPs (SNP_1, SNP_12 and SNP_13 in this study) were detected to have significant additive genetic effect on the recorded goat litter size (p < .05). The SNP_1 (NC_030809.1), a non-synonymous substitution of G for T at chr2-g. 28314990 in the exon 2 of INHA gene (NM_001285606.1), resulted in homozygote 2 (HOM2) contributed 0.25 and heterozygote (HET) contributed 0.12 larger litter than homozygote 1 (HOM1). Meanwhile, SNP_12 (Chr22-g. 11721225 A > T) and SNP_13 (Chr22-g. 11721227 A > C) (NC_030829.1) simultaneously mutated at the first and third position of a triplet AAA (lysine, K) in the exon 4 of ACVR2B gene (XM_018066623.1) had estimated genetic effects of HOM1 (0.00) and HOM2 (0.03) larger than HET (−0.12). In conclusion, one SNPs (chr2-g. 28314990 T > G) within the exon 2 of INHA and two SNPs (Chr22-g. 11721225 A > T and Chr22-g. 11721227 A > C) i n the exon 4 of ACVR2B gene were highly recommended as candidate markers of litter size in Dazu black goats. A large-scale association study to assess the impact of these variants on litter size is still necessary.     

多效疏导素对水稻秧苗素质及产量影响的研究

研究了多效疏导素水溶液对水稻秧苗素质和产量的影响。结果表明,采用适宜的浓度穴10‰雪多效疏导素水溶液对水稻秧苗素质有显著提高,可提高水稻产量达8.0%;过高的浓度,则会明显抑制水稻秧苗生长,造成减产。     

分散松香施胶剂性能影响因素的探讨

对逆转法制备分散松香施胶剂过程中的主要因素如乳化剂的种类及用量、马来松香加合物含量、搅拌转速、加料速度和乳化温度等系统地进行了探讨，提出了合理的工艺条件，并对实际生产中可能出现的问题及解决办法进行了阐述。     

波尔山羊杂交后代体尺、体重与肉用性能的相关性研究

本文利用 SPSS数据处理软件 (10 .0版 )统计分析了 2 3只 (约 8～ 9月龄 )波尔山羊与唐山奶山羊杂交后代公羊的体尺、体重和肉用性能。结果表明 :(1)波尔山羊与唐山奶山羊杂交 F1 代体尺、体重与肉用性能指标的变异程度大于 F2 代 ,其中宰前重、胴体重、净肉重和胸宽四项指标变异程度在 F1 和 F2 代中都很大 ;(2 )杂交 F1 代、F2 代各项指标 t检验结果 ,除胸宽外其余指标差异显著或极显著 ;(3 )波尔山羊杂交后代中胸宽与各项指标间相关不显著 ,宰前重、胴体重、净肉重与体尺指标相关显著或不显著。     

猪血浆转铁蛋白与部分经济性状关系的研究——Ⅰ. 多态性与产仔数和日增重的关系

用经改进的聚丙烯酰胺凝胶电泳铁染法,测定了二花脸猪血浆转铁蛋白多态类型,分析了其基因型频率和基因频率,并探讨了猪血浆转铁蛋白多态性与产仔性能和增重速度的相关性。     

Reproductive roles predict sexual dimorphism in internal and external morphology of lake whitefish,Coregonus clupeaformis

Abstract – The different reproductive roles of the sexes can predict the direction and magnitude of sexual dimorphism of external and internal morphology. Males should have enlarged structures that enhance the acquisition of mating opportunities, whereas females are predicted to have enlarged organs that are associated with the production of eggs. We tested these predictions in male and female lake whitefish, a species in which both sexes have similar overall body size and shape. After controlling for body size, male lake whitefish had significantly longer jaws and pectoral and pelvic fins, larger hearts, and more muscle than females. Sexual dimorphism in relative muscle mass may be one of the most fundamental morphological differences between males and females. Females had relatively heavier livers than males. Because the liver is important for the breakdown of fats and vitellogenesis, selection should favour an enlarged liver in females for the processing of energy and the production of large numbers of eggs.     

苹果组培芽露地嫁接技术研究

提高苹果组培芽露地嫁接成活率的关键技术措施,主要有三个方面:培育壮芽,嫁接前对组培芽进行3至5天通风锻炼,增强其对外部环境条件的适应性;正确选择砧木及嫁接部位和方法,尽可能缩小砧穗间形态结构及木质化程度的差异,人为创造适宜的环境条件,搭棚遮荫避免阳光直射,适时灌水增加土壤及空气湿度,滴注激素促进接口愈合.本试验组培芽露地嫁接成活率达到93.3%,枝条最大月生长量为38厘米.     

APPLICATION AND EFFECTS OF RARE-EARTH ON SEEDLINGS

In order to improve the quality of seedlings and economic benefits,rare--earth experiments were carried out on eleven seedling-age-types of four silvicultral treespecies.Some indexes such as the increment,biomass and chlorophyl content weredetermined.The results showed that rare-earth can improve the growth in height anddiameter,increase the bioprductivity and increase the mean benefit by887766.29 yuan/hm~2.It is an effective method to improve the quality of seedlings for the characters such as lowcost and easy grasping.And the method can be widely used in seedling.