Studies on mutlispecific resistance of gastrointestinal nematodes to benzimidazoles on dairy-goat farms

Multispecific resistance to benzimidazoles was studied in three selected farms. These farms had bred dairy goats for more than 15 years. The helminths were introduced with the goats at the establishment of the farms which afterwards remained isolated. Nematode resistance could then be related to their own management practices. Faecal egg count tests and egg hatch assays were performed to assess intensity of resistance. The generic (infective larvae in faecal cultures) and specific richness (adult worms) were assessed. The resistant species were Trichostrongylus colubriformis, Teladorsagia circumcincta, Haemonchus contortus and Oesophagostomum venulosum. Faecal egg count reduction tests and egg-hatch assays did not match exactly. Faecal larval counts after treatments gave a distorted picture of multispecific resistance: Haemonchus and Oesophagostomum were very largely over represented. The number of species found in the three farms was relatively low compared with other reports in goat farms of the area. This reduction of diversity might also be due in part to characteristics of breeding management and history (use of permanent pasture and introduction of goats at the establishment of farm).     

中国结缕草属(Zoysia spp.)植物抗寒性评价

在广泛收集结缕草属(ZoysiaWild.)种质资源基础上,按照形态类型和地理分布,选取40份种质,采用电导法(EL)对其抗寒性进行初步评价。以半致死温度(LT50)为评价指标,在参试材料中除大穗结缕草和来自台湾岛海边沙地的沟叶结缕草外,其它种质都较天堂-419(对照)抗寒;在结缕草属内,抗寒性具有明显的种间差异,耐寒性依次为日本结缕草>中华结缕草>沟叶结缕草>细叶结缕草>长花中华结缕草>大穗结缕草,其中细叶类型的沟叶结缕草和细叶结缕草抗寒性变异较大;日本结缕草和中华结缕草抗寒性变异与地理分布之间没有显著关系,但分布在海边的种类和种质抗寒性明显较低;结缕草属及其中华结缕草的抗寒性与叶长、叶宽以及叶背面被毛之间均存在显著的负相关关系,但这种关系不体现在日本结缕草上。实验结果还表明,EL方法是评价结缕草属抗寒性较为可靠的方法之一。     

海南半干旱地区芒果间作柱花草及作物效益初探

1998～2001年在海南东方干旱半干旱燥红土芒果与柱花草及作物间作示范区的实践表明，在芒果园间作扁豆、柱花草、番薯和花生可显著提高芒果挂果率，芒果收入比芒果单种分别增加97．79％、98．53％、54．41％和48．28％，果园综合收入比芒果单种分别增加108．55％、98．53％、97．98％和91．58％，同时，果园间作可显著提高果园土壤有机质、全氮、速效磷的含量和土壤pH值，间作扁豆使土壤有机质含量增加53．3％，间作柱花草使土壤全氮增加43．92％，速效磷增加78．16％，从而达到了以短养长、增加农民收入、提高土壤肥力和持续管理利用的目的。     

大岛樱病虫害及其防治的研究

本文对大岛樱中国引种点危害较严重的４种病害和２种害虫进行观测和种类鉴定，指出了各病虫害的危害症状和发生发展规律等，并试验或介绍了相应的防治措施。     

中国梅的变异与分布研究

通过文献阅读、标本查阅及实地考察，发现了梅的一些变异类型。提出了梅的地理分布格局，对梅分布的历史迁作的探讨。种、滇、藏交界的横断山区是梅的自然分布中心，另外还有一些亚中心；在对种群数量和变异类型研究的基础上，确认梅的自然分布中心即梅的变异中心。     

基于果实相关性状的桃品种初级核心种质取样策略研究

以国家种质资源圃(北京)编目的558份桃品种的18个形态学和农艺学性状为基本数据,研究了桃品种初级核心种质构建的取样策略,包括总体取样比例的确定及取样方案的选择,以获得最佳的初级核心种质。本试验共设10%～90%9个总体取样比例;取样方案研究包括分组原则和组内取样比例的确定。结果表明,桃初级核心种质的适宜总体取样比例为10%;按品种类群分组结合多样性比例取样为构建桃初级核心种质的最佳取样方案;利用此取样策略从558份桃品种中提取56份作为核心样本,对其代表性进行检测表明所构建的初级核心种质能够很好地代表桃原始种质的遗传多样性。     

Acquired resistance triggered by elicitins in tobacco and other plants

Elicitins are a family of proteins excreted byPhytophthora spp. They exhibit high sequence homology but large net charge differences. They induce necrosis in tobacco plants which then become resistant to the tobacco pathogenPhytophthora parasitica var.nicotianae. In stem-treated plants, resistance was not restricted to the site of elicitin application, but could be demonstrated by petiole inoculation at all levels on the stem. Resistance was already maximum after two days and lasted for at least two weeks. It was effective not only towardsP. p. var.nicotianae infection, but also against the unrelated pathogenSclerotinia sclerotiorum. In contrast to dichloroisonicotinic acid, an artificial inducer of systemic acquired resistance, which was increasingly effective with doses ranging from 0.25 to 5mole per plant, the basic elicitin cryptogein exhibited a threshold effect, inducing near total resistance and extensive leaf necrosis above 0.1 nmole per plant. Between 1 and 5 nmole, acidic elicitins (capsicein and parasiticein) protected tobacco plants with hardly any necrotic symptom. Elicitins exhibited similar effects in various tobacco cultivars andNicotiana species, although with quantitative differences, but induced neither necrosis nor protection in other SolanaceÆ (tomato, petunia and pepper). Among 24 additional species tested belonging to 18 botanical families, only some BrassicaceÆ, noticeably rape, exhibited symptoms in response to elicitins, in a cultivar-specific manner. Elicitins appear to be natural specific triggers for systemic acquired resistance and provide a tool for unraveling the mechanisms leading to its establishment.Abbreviations AR acquired resistance - HR hypersensitive response - INA 2,6-dichloroisonicotinic acid - Ppn Phytophthora parasitica var.nicotianae - SAR systemic acquired resistance     

灵芝多糖与三萜类的提取纯化工艺研究进展

多糖和三萜两大类活性物质是灵芝的主要药效成分.本文综述了从灵芝子实体、菌丝体和孢子中提取纯化灵芝多糖和三萜类物质的提取纯化工艺研究现状、研究趋势及研究内容.     

蝴蝶兰组织培养中的褐化控制研究

 系统研究了吸附剂(活性炭、PVP) 与柠檬酸、半胱氨酸、谷胱甘肽、维生素C和硫代硫酸钠5种抗氧化剂对蝴蝶兰组培中褐化的影响。结果表明: 活性炭能有效控制褐化和促进生长, 但不利于分化; 谷胱甘肽控制褐化的效果虽不及活性炭, 但综合效果最佳。