Gentamicin Nephrotoxicity – A Comparison of In Vitro Findings with In VivoExperiments in Equines

The aminoglycoside gentamicin is often used in equine practice. Despite its clinical use, concerns remain regarding the potential toxic side-effects, such as nephrotoxicity, in equine patients, particularly after repeated dosing. The aim of the study was to investigate first in vitro the mechanisms contributing to the renal toxicity of gentamicin and to identify sensitive biomarkers indicating proximal tubule damage. To this end, the kidney-derived cell lines LLC-PKI and MDCK were treated with gentamicin at different concentrations. Toxicity was assessed by measuring the release of gamma-glutamyl transferase (GGT), and the production of reactive oxygen species (ROS). Cell viability was measured using Alamar blue (AB) and Neutral red (NR) cytotoxicity assays. Gentamicin exerted a dose-dependent toxicity. Primarily, loss of brush border membrane integrity, indicated by GGT leakage, and an increased ROS production were observed. As GGT was found to be a sensitive marker for gentamicin-induced renal cell injury, in the subsequent in vivo experiments, in which ponies were given gentamicin (3.0 mg/kg bw three times daily and 4.5 mg/kg bw twice daily) for five consecutive days, plasma levels and the urinary excretion of GGT and creatinine were measured and the GGT:creatinine ratio was calculated. Elevated GGT levels in urine following gentamicin therapy were observed, but this enzyme leakage was transient and returned to baseline values after cessation of therapy. It could thus be concluded that even a conservative dose regimen of gentamicin did not result in significant renal toxicity in healthy ponies.     

MC812和郑单958子粒灌浆和脱水特性研究

以MC812和郑单958为试验材料,分析其子粒灌浆和脱水特性,研究不同基因型玉米品种子粒灌浆和脱水特性的差异。结果表明,百粒干物重在品种间存在显著差异,MC812平均百粒干物重较郑单958高2.64 g。与郑单958相比,MC812子粒达到最大灌浆速率的时间(Tmax)平均提前4.40 d,灌浆起始势(R0)高0.25,活跃灌浆期(P)长1.33 d。MC812灌浆渐增期的平均灌浆速率显著高于郑单958,灌浆缓增期则与郑单958相当。MC812生理成熟时子粒含水量略低于郑单958,但差异不显著。收获时的子粒含水量在品种间呈极显著差异,MC812收获时的子粒含水量则较郑单958低5个百分点。生理成熟后的脱水速率在品种间呈极显著差异,MC812分别较郑单958高0.15%/d。穗粒数、百粒重和产量在品种间呈极显著差异,MC812产量较郑单958高6.67%、穗粒数和百粒重分别较郑单958高8.56%和8.04%。     

ADuC812单片机在油田地面仪器辅助系统中的应用

为使油田地面仪器辅助系统运行可靠,操作直观、简便,系统应包括直流电源和综合控制箱等,以使系统具有直流电源给井下仪器自动供电,综合控制箱实现内外缆芯的切换、仪器信号截取、供电通道的选择和强电的隔离等功能。基于以上这些考虑,采用ADuC812芯片设计了油田地面仪器的程控辅助系统。该系统采用主从式通讯方式,上位机作为主机,程控直流电源和综合控制箱作为从机,主机和从机之间通过标准的RS-232接口实现双向串口通讯。     

Synchronization of flowering in maize (Zea mays L.) by the genetic system pg11 pg12

Summary The mutant genotype pg11pg11 pg12pg12-epistasis of double recessive factors-determines greater precocity of flowering. The possibility of using this mutant in the production of hybrid seed as a mean of synchronizing flowering in parent strains of different earliness is studied. The differences between mutant and green control genotypes in the characters of days to pollen shedding, pollen production, and height of plants are evaluated. Four mutant lines are considered together with their respective green near-isolines in two distinct localities and across four successive plantings.The mutant near-isolines presented lower values than those of the green near-isolines for all the characters. The maximum reductions in days to flowring were obtained in early plantings and late maturity strains. The reduction in pollen production was about 50%. The reduced height of the mutants is explained, at least in part, by the correlation of this factor with days to flowering. The mutants did not prove to be more sensitive to the environment than their green near-isolines. Seed production was not studied since the mutant is very deficient for this character and it could therefore only be utilized as the male parent. We concluded that the mutant, could be used to synchronize flowering in the industrial production of hybrid seed.     

虚拟仪器技术在疲劳试验机功能改进中的应用研究

为了提高SVF-1E-01型电液伺服拉压疲劳试验机的测试精度、简化试验操作，应用虚拟仪器技术对其进行了改造。采用研祥工控集团的PCL-812pg多功能数据采集接口卡，在WIN98操作系统平台下，用面向对象的可视化编程环境Delphi5.0集成，设计了虚拟示波器及虚拟信号发生(合成)器。改进后的系统功能包括：模拟信号的采集与显示、单一及多路合成信号的产生及输出等。软件系统具有形象、直观、操作方便等特点。试验结果表明：控制信号精度较高，可以满足试验的要求。整个软、硬件系统接入拉压疲劳试验机中，完成该疲劳试验机     

甘蓝型油菜优质高产新品种冠油杂812选育

[目的]为双低甘蓝型春油菜杂交种冠油杂812的大面积推广及新品种选育中的应用提供参考。[方法]冠油杂812是以不育系9511A为父本、恢复系3219R为母本,采用细胞质雄性不育三系法育成的中早熟甘蓝型双低油菜杂交种。[结果]2010~2011年在甘肃省春油菜区域试验中,冠油杂812在2年12点(次)中,相比对照有9个点增产,3点减产,增产幅度1.93%~10.11%,折合平均产量为3 506.25 kg/hm2,比对照陇油5号平均增产10.08%。其中,2010年6点(次)折合平均产量为3 082.65 kg/hm2,比对照陇油5号增产8.18%;2011年6点(次)折合平均产量为3 919.70 kg/hm2,比对照陇油5号增产2.08%。冠油杂812籽粒含油率45.66%,芥酸含量0.1%,硫苷含量7.2 mol/g,芥酸和硫苷达到国家双低油菜标准。[结论]冠油杂812综合性状优良,高产优质,适宜在甘肃省海拔2 500m以下有灌溉条件的春油菜区及类似生态区种植。     

杂交水稻新品种天优812在龙岩种植表现及高产栽培技术

天优812系湖南亚华种业科学研究院以广东省农科院水稻所选育的不育系天丰A为母本和自选恢复系华恢812为父本配组育成的三系杂交晚稻新品种。在龙岩作晚稻种植,表现株型适中、群体整齐、分蘖力中等、后期转色好、熟期适中、耐肥抗倒、抗稻瘟病、米质优等特点。主要介绍了其在龙岩的种植表现及高产栽培技术。     

黄瓜新品种东农812的选育

东农812是以D1158为母本,以D0708-2为父本育成的适于我国北方地区保护地栽培的优质、抗病、高产鲜食型旱黄瓜一代杂种。植株长势强,耐低温、弱光,高抗枯萎病,抗霜霉病和白粉病。第1雌花节位为第4~5节,强雌性。果实短圆筒形,果皮翠绿色,瓜长15~18 cm,粗3.5~4.0 cm,单瓜质量120~140 g。商品瓜率90%以上,优质丰产,每667m2产量4 000 kg左右,适合我国东三省及内蒙古地区棚室春、秋两季栽培。     

二株副鸡嗜血杆菌分离株的生物学特性鉴定

本试验对从广西分离的４株疑似副鸡嗜血杆菌（Ｈ．ｐｇ）（ＧＸ－９５,ＧＸ－９７,ＧＸ－９８－１,ＧＸ－９８－２）进行生物学特性研究。根据生物学特性,ＧＸ－９７和ＧＸ－９８－１被定为Ｈ．ｐｇ,ＧＸ－９５和ＧＸ－９８－２的生化反应特性有明显差异,用标准Ｈ．ｐｇ血清未能定型。交叉血凝抑制（ＨＩ）试验显示,各菌株之间的交叉ＨＩ效价达１６０,但各菌株与其本身的抗血清ＨＩ效价达１２８０,显示出很强的菌株特异性。抗菌素敏感试验也显示ＧＸ－９７、ＧＸ－９８－１和参考菌株的药敏谱更加相似。