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以岩溪晚芦(Citrus reticulata Blanco)为试材,在果实成熟期用乙烯诱导果蒂的离层,分时段应用探针组(Probe Set ID)总数至少30302的基因芯片研究了离层基因表达情况,且同步进行了无乙烯诱导的对照实验,得到芯片两两比较结果。4h、12h和24h样品处理同样品对照的两两比较结果表明:4h、12h和24h样品处理分别至少有650个、500个和2200个探针组比样品对照显著下调表达,分别占探针组总数的2.15%、1.65%和7.26%;4h、12h和24h样品处理分别至少有550、600和1200个探针组比样品对照显著上调表达,分别占探针组总数的1.82%、1.98%和3.96%。研究首次证实了Affymetrix公司的柑橘基因表达检测芯片可应用于中国岩溪晚芦,首次发现了至少500条基因连续20h的表达趋势。 相似文献
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Here we report the adaptation optimization of an efficient accurate inexpensive assay that employs custom-designed silicon-based optical thin-film biosensor chips to detect unique transgenes in genetically modified 《分子植物育种》2007,5(2):241-241
Here we report the adaptation and optimization of an efficient, accurate and inexpensive assay that employs custom-designed silicon-based optical thin-film biosensor chips to detect unique transgenes in genetically modified (GM) crops and SN-P markers in model plant genomes. Briefly, aldehyde-attached sequence-specific singlestranded oligonucleotide probes are arrayed and covalently attached to a hydrazine-derivatized biosensor chip surface. Unique DNA sequences (or genes) are detected by hybridizing biotinylated PCR amplicons of the DNA sequences to probes on the chip surface. In the SN-P assay, target sequences (PCR amplicons) are hybridized in the presence of a mixture of biotinylated detector probes and a thermostable DNA ligase. Only perfect matches between the probe and target sequences, but not those with even a single nucleotide mismatch, can be covalently fixed on the chip surface. In both cases, the presence of specific target sequences is siL, nified by a color change on the chip surface (gold to blue/purple) after brief incubation with an anti-biotin IgG horseradish peroxidase (HRP) to generate a precipitable product from an HRP substrate. 相似文献
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Effect of simulated transport stress on the rat small intestine: A morphological and gene expression study 总被引:2,自引:0,他引:2
Changrong Wan Peng Yin Xiaolong Xu Mingjiang Liu Shasha He Shixiu Song Fenghua Liu Jianqin Xu 《Research in veterinary science》2014
The present study investigated the effects of simulated transport stress on morphology and gene expression in the small intestine of laboratory rats. Sprague Dawley rats were subjected to 35 °C and 0.1×g on a constant temperature shaker for physiological, biochemical, morphological and microarray analysis before and after treatment. The treatment induced obvious stress responses with significant decreases in body weight (P < 0.01), increases in rectal temperature, serum corticosterone (CORT), serum glucose (GLU), creatine kinase (CK) and lactate dehydrogenase (LDH) levels (P < 0.01), as well as expression of Hsp27/70/90 mRNA (P < 0.05; P < 0.01). The rat jejunum was severely damaged and apoptotic after mimicking transport stress, which may mainly be related to cell death, oxidation reduction and hormone imbalance determined by microarray analysis. The bioinformatics analysis from the present study would provide insight into the potential mechanisms underlying transport stress-induced injury in the rat small intestine. 相似文献
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