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1.
Fa-chao SUN Min TAN Yuan-chao ZHANG Yu-chao WANG Sheng-liang CAO Guo-fei DING Fang-yuan CONG Li-hong GUO Si-dang LIU Yi-hong XIAO 《农业科学学报》2019,18(7):1436-1442
To investigate the epizootic of swine influenza virus(SIV), 60 nasal swabs were collected from a clinical cases of pig farm in Tai'an City, Shandong Province of China in April 2017. SIV was isolated by inoculating into 10-day-old Special Pathogen Free embryonated eggs and the whole genome was sequenced. An H1N1 subtype SIV was isolated and designated as A/swine/Shandong/TA04/2017(H1N1). Phylogenetic analysis showed that apart from the polymerase A(PA) fragment belonging to the 2009 pandemic H1N1 branch, seven genome segments belonged to avian-like H1N1 influenza virus lineage. The cleavage site sequence of the hemagglutinin(HA) protein was PSIQSR↓G, which is a typical molecular biological characteristic. Five potential N-glycosylation sites(N14, N26, N277, N484 and N543) were found in the HA gene. To further investigate the epidemiology of SIV in this farm, the 995 serum samples were assessed with EAH1N1 2009 pandemic H1N1 and H3 N2 antigens. The results showed that the total positive rate was 65.43%. The positive rates of single virus infection detected by EAH1N1, 2009 pdmH1N1 and H3 N2 for serum HI(Hemagglutination inhibition) were 48.35, 30.85 and 7.47%, respectively. The results showed that SIV in Shandong Province has been reassorted in some segments and the SIV-positive rate was high on the SIV outbreak farm. These data provide evidence of an epizootic of SIV. 相似文献
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优化了S1核酸酶突变检测体系,结果表明(1)扩增法比杂交法产生异源双链DNA更节省时间;(2)PCR产物可以直接作为突变检测的底物;(3)适当降低反应温度、适当增加反应缓冲液中的NaCl浓度、适当缩短反应时间可提高突变检测效果。 相似文献
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本文报道了寄生在雷林一号桉“[Eucalyptus leichou NO:1]”叶片上的一个国内新记录病原菌[Fairmaniella leprosa(Fairman)Petrak & Sydow.]的主要特征,并与已知种作了比较;文中讨论了国内外研究状况,寄主及分布。标本采集于广东省雷州林业局林科所人工桉树林。 相似文献
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蜂乐1号烟剂是一种最近研制成功的新型杀蜂螨药剂,其有效成分为溴螨酯.经室内外试验结果,对蜂螨有较强杀伤力,对蜜蜂十分安全.烟剂中的溴螨醋对雅氏大蜂螨的LC_(50)为0.26克/米~3,LC_(95)为2.92克/米~3 对蜜蜂则分别为71.45克/米~3和218.70克/米~3,二者分别相差274.8倍和74.9倍.一平箱蜂群一次用含0.45克有效成分的药,对蜂体上大蜂螨的防除效果平均93%以上.对蜂群每隔5天施药一次,经3次施药后蜂群状况正常.在施药3次后对所取蜜样进行检测,其中溴螨酯的残留量远远低于联合国粮农组织所规定的参考限量.该烟剂适于在蜂箱外施药,比目前国内一般的开箱施药方法提高工效15倍以上,更适用于许多过去不便用药的场合. 相似文献
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Although the molluscicide Frescon is a strong neurotoxin to the Lymnaea stagnalis central nervous system in vitro, it is probable that the exposure of the whole animal to this molluscicide fails to result in central nervous system abnormalities: Frescon does not appear to reach the brain in sufficient quantity to disrupt its normal activity. However, only those Frescon analogs found to be neurotoxic were molluscicidal, suggesting some related mode, if not site, of action. Frescon and its analogs may act by affecting excitable tissues other than the nervous system (e.g., the snail musculature) by altering certain functional and/or structural membrane properties. 相似文献
8.
C. Rusterucci Z. Zhao K. Haines D. Mellersh M. Neumann R.K. Cameron 《Physiological and Molecular Plant Pathology》2005,66(6):120
As plants mature it has been observed that some become more resistant to normally virulent pathogens. The ability to manifest the Age-Related Resistance (ARR) response in Arabidopsis to Pseudomonas syringae pathovars tomato (Pst) coincided with the transition to flowering in plants both delayed and accelerated in the transition to flowering. ARR was also associated with a change in PR-1 gene expression, such that young plants expressed PR-1 abundantly at 3 days post inoculation (dpi) while mature plants expressed much less. The Arabidopsis ARR response requires SA accumulation via isochorismate synthase (ICS1) [24]. ICS1 was expressed one dpi with virulent and avirulent Pst in both young and mature plants. The ARR response was also effective versus avirulent Pst providing an additional 4-fold limitation in bacterial growth. Arabidopsis ARR was found to be ineffective against two necrotrophs, Erwinia carotovora subspecies carotovora (bacterium) and Botrytis cinerea (fungus) and one obligate biotroph, Erysiphe cichoracearum (fungus). However, mature wild type, SA-deficient sid2 and NahG plants supported little growth of the obligate biotrophic oomycete, Peronospora parasitica. Therefore ARR to P. parasitica appears to be SA-independent, however the level of ARR resistance was somewhat reduced in these mutants in some experiments. Thus, there may be numerous defence pathways that contribute to adult plant resistance in Arabidopsis. 相似文献
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