Apparent seroprevalence,isolation and identification of risk factors for brucellosis among dairy cattle in Goa,India

Brucellosis is a highly contagious zoonotic infection affecting livestock and human beings. The disease has been reported worldwide except in few countries where it has been eradicated. The prevalence of brucellosis among cattle from 11 farms having a history of abortions was studied. A total of 481 samples comprising of blood, milk, vaginal swabs, vaginal discharges, placental tissues and fetal tissues were collected from 296 animals. Clinical samples were processed for the isolation of Brucella. Serum samples (n = 296) were tested by Rose Bengal Plate Test (RBPT) and indirect ELISA. A total of 90 (30.40%) and 123 (41.55%) samples were positive by RBPT and indirect ELISA, respectively. Also 27.02% samples were positive by both the tests. Brucella isolates (n = 8) were recovered from clinical samples using Brucella selective media. All the isolates demonstrated PCR amplification for the bcsp31 and IS711 genes. Amplification of Brucella abortus specific primer was demonstrated by all the isolates in AMOS PCR indicating isolates to be of either B. abortus biotype 1, 2 or 4. Risk factors for transmission of brucellosis among cattle population were studied by field surveys. It was observed that lack of awareness about brucellosis (OR = 8.739, P = 0.138) and inadequate floor space (OR = 0.278, P = 0.128) were crucial risk factors for transmission of bovine brucellosis.     

AMOS PCR在布鲁氏菌种型鉴定中应用的研究

AMOSPCR是近年来在布鲁氏菌上尝试使用的一种布鲁氏菌检测方法,可作为布鲁氏菌诊断及菌种类型鉴定的PCR方法,可区分牛种布鲁氏菌1、2、4型,羊种布鲁氏菌、猪种布鲁氏菌、绵羊附睾种布鲁氏菌。本试验用来自国内的疫苗S2,M5及国际上常用的布鲁氏菌疫苗S19,104M试验AMOSPCR的效果,同时对AMOSPCR扩增条带进行测序。结果表明,除国内布鲁氏菌S19检测结果与国外菌株不同外,其余的几种疫苗株都得到典型的特征性条带。同时测序结果也表明各种属条带无交叉反应,为今后国内诊断布鲁氏菌病诊断方法研究指出一个方向。     

冬小麦返青后腾发量时空尺度效应的通径分析

作物腾发量（ET）的时空尺度效应是作物高效用水调控与节水灌溉管理中需面对的基础科学问题。该文对返青后冬小麦生育期内试验小区实际腾发量（ETa）和区域水分通量（LE）以及作物生长环境因子进行实测,利用通径分析方法,对冬小麦ET时空尺度效应及其主要影响因子进行分析。结果表明,不同时间尺度和空间尺度下,作物蒸腾蒸发的影响因子不同,显示了其不同的时空尺度效应。对试验小区实际腾发量ETa来说,以全日24 h的数据来分析,其主要影响因子是叶面积指数LAI和净辐射Rn,而白日时段（7:00-18:00）分析显示主要影响因子是空气饱和水汽压差VPD_7-18和叶面积指数LAI。对田间尺度的区域水分通量LE来说,全日24h数据的主要影响因子是净辐射Rn和作物高度H,白日时段（7:00－18:00）数据的主要影响因子是饱和水汽压差VPD_7-18和作物高度H。冬小麦返青后的时间尺度效应表现是全日24h作物腾发量的主要影响因子是净辐射,而白天时段影响腾发量的主要因子是空气饱和水汽压差;空间尺度效应表现是小面积的作物腾发量大小对作物的叶面积指数变化敏感,区域水分通量的大小与下垫面植被高度的变化有关。     

农业院校硕士研究生幸福感影响因素分析——以江西农业大学为例

通过运用AMOS软件,对江西农业大学在读硕士研究生的幸福感现状调查数据进行分析,得到测量模型的路径图。发现影响硕士研究生幸福感的主要因素包括：个性因素、人际交往因素、学业期待因素、专业因素、学校因素、就业因素、家庭因素等,其中就业因素与个性因素对研究生幸福感的影响最大。为此,农业院校应加强培养硕士研究生就业能力．努力拓展就业渠道;把握硕士研究生群体个性特征,引导学生完善自我个性;建立研究生心理健康档案．引导学生健康发展。