Vitrification of mouse two-cell and blastocyst stage embryos in simplified closed system using either a hemi-straw or a hollow fiber device

Two-cell stage and blastocyst stage mouse embryos were equilibrated in a medium containing 7.5% ethylene glycol (EG) and 7.5% dimethyl sulfoxide (DMSO) for 8–15 min. Vitrification was performed in a medium containing 0.5 M sucrose and either 15% EG + 15% DMSO, 17.5% EG + 17.5% DMSO, or 20% EG + 20% DMSO for 30 s. They were then placed either on a hemi-straw (HS) or a hollow fiber vitrification (HFV) device and vitrified by cooled air inside a 0.5-ml straw. In two-cell embryos, a 100% survival rate was obtained from all groups except the 20% HS group (P > .05). All vitrified two-cell groups showed similar rates of blastocyst development to that of fresh control group (P > .05), except 17.5% and 20% HFV groups, which were significantly lower than the other groups (P < .05). In the blastocyst embryos, the HFV groups were divided into two subgroups (non-collapsed; HFV-NC and collapsed; HFV-C blastocyst). Re-expansion rate in 15% HFV-NC, 17.5% HFV-NC, and 15% HFV-C groups was reduced (P < .05), whereas the rest were similar to control. In conclusion, we established a simplified, reliable, and closed system for HFV vitrification applying hemi-straw, which does not require skilled practitioners.     

生长激素释放肽-6缓释微球的制备及其对动物生长的影响

以生物降解材料乳酸乙醇酸共聚物(PLGA,LA-GA 8515)为载体,采用复乳-液中蒸发法制备含生长激素释放肽-6的乳酸乙醇酸共聚物微球,并通过体外药物释放试验评价载药微球的释药特征.结果得到收率、粒径大小和分布及含药量均满意的微球,其中包封率为100%,平均体积径为3.45μm,收率为79%.在37℃、pH7.0的生理等渗缓冲液中,首日释药19.19%,其后以平均日释药3.23%的速度释药25 d.小鼠肌肉注射微球30 d后,累积增重比注射生长激素释放肽-6、生理盐水分别高22.56%(P<0.05)和53.17%(P<0.01).结果表明,生长激素释放肽-6乳酸乙醇酸共聚物微球有望发展成为新型长效制剂.     

河南省承接产业转移的现状和特点

本文就河南省承接产业转移的需要,把快速调整产业结构、实现跨越式发展的现实,区域间的产业梯次分布转移的前提,河南省承接产业转移的时机条件,承接产业转移的势头,以及面临的差距、面临的竞争形势、区域竞争形势进行了阐述。     

Bubbleless Aerated Membrane Bioreactor for Ramie Degumming Wastewater Treatment

Through introducing the principle of bubbleless aerated membrane bioreactor, the traditional membrane bioreactor was improved. It could be obtained that the removal efficiency of COD and NH3 - N in bubbleless aerated membrane bioreactor increased 4% and 2% than that in traditional membrane bioreactor, respectively. Adopting bubbleless aeration as reflushing air which not only increased DO in the MBR, but also advanced the oxygen utilization, and the energy consumption decreased as well.     

基于词袋特征的空心村高分影像建筑物解译模型

如何利用高分影像构建自动解译模型是快速高效获取空心村建筑物的关键,对空心村调查研究具有重要意义。针对传统目视解译需要专业知识,效率低、工作量大的问题,提出一种基于词袋特征的空心村高分影像建筑物解译模型。首先,对比了多种影像特征提取方法;然后,选取词袋特征(Bo W)和支持向量机(SVM)构建建筑物自动解译模型;最后,为检验方法的有效性,选取空心村高分影像构建了建筑物样本库,并基于该样本库进行实验研究。结果表明本文方法的分类准确度可以达到0.86,所提方法可用于空心村内建筑物自动解译,具有较高的实用价值。     

基于卷积神经网络的空心村高分影像建筑物检测方法

基于卷积神经网络(CNN)提出了一种适用于空心村高分影像的建筑物自动检测方法,该方法利用多尺度显著性检测来获取包含建筑物信息的显著性区域,然后通过滑动窗口获取显著性区域内目标样本块,再将这些样本块输入训练好的CNN并结合SVM来实现分类。为检验方法有效性,选取高分影像进行实验,结果表明,显著性检测能够有效地获取主要目标,减弱其他无关目标的影响,降低数据冗余;卷积神经网络能够自动学习高层次的特征,基于CNN对高分影像进行建筑物检测,分类准确度可以达到97.6%,表明该方法具有较好的鲁棒性和有效性。     

一种新型节能保温墙体的研究

随着经济的快速增长,能源短缺等相关问题已经越来越严重,建筑节能问题已成为可持续发展的重要保证。该文介绍了现今我国在保温墙体应用上所采取的一些方法和措施,墙体保温的形式及特点;并重点论述了新型环保节能型材料玻化微珠在墙体保温中的应用前景。     

含淀粉反相乳液及交联淀粉微球制备

[目的]研究影响含淀粉反相乳液稳定性的因素,优化交联淀粉微球制备工艺。[方法]采用单因素法研究影响反相乳液稳定性的主要因素,用正交试验法确定了合成交联淀粉微球的最佳工艺。[结果]使用0.6%的Span60（HLB=4.70）,油、水相体积比为3∶1,水相中淀粉浓度为16%时可形成稳定的W/O型含淀粉反相乳液;制备微球的最佳工艺条件为：油、水相体积比4∶1,淀粉乳浓度16%,交联剂用量3 ml,反应温度45℃,乳化剂用量0.3%;微球产品近似球状,球体表面粗糙,粒径分布均匀,平均粒径15μm。[结论]经过优化的制备工艺能够合成粒径均匀的交联淀粉微球。     

大孔吸附树脂联用KB-2微球柱层析法分离纯化虫草素的工艺优化

通过比较19种大孔吸附树脂对虫草素的解吸附率并联用KB-2微球柱层析法优选出一种简单快捷分离纯化蛹虫草(Cordyceps militaris)培养基中虫草素的制备工艺。结果表明,经大孔吸附树脂XDA-8D吸附后,再用体积分数30%的乙醇解吸附,虫草素纯度从原料中的0.4%达到了10.53%;联用KB-2微球柱层析法分离虫草素,可将纯度提升至66.94%,其优化后的参数为:上样流速1.0 mL/min,上样浓度0.5 mg/mL,用300 mL(2VB)水和体积分数10%的乙醇450 mL(3VB)除杂,30%乙醇1500 mL(10VB)洗脱得到虫草素。