饥饿、季节及性别对大鲵肌肉、肝脏生化成分的影响

季节会影响到大鲵肌肉的脂肪及钙的含量,肝脏的脂肪、钙及游离氨基酸的含量,饥饿对肌肉的酸化成分不产生影响,但饥饿程度的增加,会对肝脏脂肪无氮浸出物及游离氨基酸产生影响;繁殖前雌性大鲵的肌肉肝脏的肪肪含量均高于雄性的。     

Annual sex hormonal profiles, gonad development and age determination of the Mekong giant catfish (Pangasianodon gigas, Chevey)

Annual sex hormonal profiles, gonad development and age determination of 18 (13–14 kg body weight) and three (145–226 kg body weight) Mekong giant catfish (MGC) (Pangasianodon gigas, Chevey) reared in earthen ponds in Chiang Mai and from the Mekong River in Chiang Rai, Thailand, respectively, were investigated. The hormonal profiles were determined from blood samples of the fish by electrochemiluminescence immunoassay during May to August in 2000. The highest testosterone levels of 0.06 ng mL^?1 in both sexes and the highest oestradiol of 47.8 and 14.23 pg mL^?1 in females and males, respectively, were observed in May. The gonadosomatic index was found to be 0.07 for males and 0.38 for females from ponds in comparison with 2.27 for males and 8.29 for females from the Mekong River. Higher development stages of spermatocytes and oocytes of the cultured fish in May than in February and November were demonstrated. No mature germ cells were obtained from either the males or females, indicating no sexual maturity. The average age of fish was determined from the annual rings of the cross‐section of the pectoral fins by a stereomicroscope. The average age of fish in earthen pond determined from the annual ring was 8 years, which agreed with the actual culture records, while those from the Mekong River were 21 years. This information will be beneficial for the breeding programmes and conservation of the MGC.     

三种融合标签对大熊猫轮状病毒结构蛋白VP6-VP7可溶性表达的影响

大熊猫轮状病毒(giant panda rotavirus,GPRV)是引起幼龄大熊猫腹泻的主要病原,对圈养大熊猫产生了较大的危害。轮状病毒结构蛋白VP6是一种载体蛋白,可介导黏膜免疫反应。VP7是轮状病毒结构蛋白中主要的中和抗原。因此,VP6-VP7的融合表达作为候选抗原对该病的防治具有重要的意义。传统大肠埃希菌原核表达存在表达量低、可溶性差以及纯度低等弊端。本研究使用醛缩酶(EDA)、谷胱甘肽S-转移酶(GST)、麦芽糖结合蛋白(MBP)3种融合标签,以实现获得表达量高和纯度高的GPRV-VP6-VP7重组表达蛋白。将扩增的VP6、VP7基因片段利用同源重组酶构建到含3种融合标签的表达载体pET21b上,将重组质粒转化至大肠埃希菌Rosetta(DE3)感受态细胞中进行低温诱导表达。用Ni-柱亲和层析法纯化目的蛋白,SDS-PAGE和Image J分析蛋白表达量和可溶性,Western blot分析得到表达的重组表达蛋白正确且具有蛋白活性。实验结果证明,EDA标签能显著促进VP6-VP7蛋白的原核可溶性表达,提高VP6-VP7蛋白表达量。     

基于稀土超磁致伸缩材料的微致动器设计

选择Terfenol-D作为微致动器主要材料，采用弹簧对GMM棒施加一定的预压力以获得较大的磁致伸缩应变，采用偏置磁场消除GMM棒的倍频现象并使之工作在线性段，对磁路进行了分析和优化设计。针对电磁线圈工作发热及涡流损耗引起的GMM棒温升，采取了强制水冷方法并专门设计了一个恒温冷却系统用以消除材料的热变形对致动精度的影响。     

基于超磁致伸缩转换器的流体控制阀及其技术

介绍了超磁致伸缩材料在流体控制阀中的应用研究现状，详细分析了使用GMA时要解决的热补偿、微位移放大和非线性控制等几项关键技术。提出了两种基于GMA的两级电液伺服阀实现方案。     

超磁致伸缩驱动器的输出特性研究

介绍了超磁致伸缩驱动器结构及其内部磁路的设计方法，对采用国产材料研制的驱动器的位移及力的输出特性进行了测试。实验结果表明，该驱动器具有理想的动态和静态输出精度和输出范围，可以用于精密设备的低频主动隔振平台中。     

Comparison of three types of adhesives in attaching coral transplants to clam shell substrates

• 1. Nubbins from 12 coral species were transplanted onto dead giant clam shells at three sites in a lagoon near Bolinao, north‐western Philippines. Transplants were attached using three types of adhesives: cyanoacrylate glue (SG), epoxy putty (EP) and marine epoxy (ME) and were monitored over five months for detachment, in situ mortality and natural self‐attachment by tissue growth.
• 2. Corals attached with SG showed a significantly higher rate of detachment (logrank test) than those attached with either EP or ME (P<0.001 in each case). Also, those attached with EP showed a higher rate of detachment than corals attached with ME (logrank=6.46, P<0.05).
• 3. ANOVA (P>0.05) and survival analysis (logrank=2.85, P>0.05) showed no among‐adhesives differences in in situ mortality. Among the species used, Porites cylindrica, Pavona frondifera, Heliopora coerulea and Porites rus had the highest survivorship with 82%, 80%, 76% and 73%, respectively, of nubbins alive at the end of the experiment and also displayed the highest cumulative rates of self‐attachment. Species with the lowest survival rates included Acropora muricata and four pocilloporids.
• 4. Although there was no significant difference in the number of self‐attaching transplants among the adhesives, corals transplanted using ME and EP self‐attached earlier than those transplanted using SG (logrank=11.19, P<0.01).
• 5. Results from this study highlight the importance of selecting the appropriate means of attaching corals to substrates and carefully considering species‐specific traits of candidate corals for transplantation.

Copyright © 2008 John Wiley & Sons, Ltd.     

Can giant clam (Tridacna squamosa) populations be restored on Singapore's heavily impacted coral reefs?

• 1. Giant clams have been a sustainable resource for millennia, but unregulated harvesting has led to local extinctions within the Indo‐Pacific region. Giant clam mariculture can produce large numbers of juveniles for restocking wild populations where natural recruitment is low or absent.
• 2. Singapore is surrounded by more than 60 small islands, many with fringing reefs. These reefs, however, experience increased turbidity and sedimentation resulting from massive coastal development projects and regular dredging of shipping lanes.
• 3. Seven reefs off Singapore's southern islands were surveyed (9670 m²) for giant clams. Also, an experiment was conducted to determine the growth of Tridacna squamosa reared in aquaria under three light treatments: ～50% ambient photosynthetically active radiation (PAR); ～25% ambient PAR; and ～12% ambient PAR. Finally, 144 clams (T. squamosa) were transplanted to four reefs around Singapore to study survival and growth in a heavily impacted environment.
• 4. A total of 23 adult clams from three species were found during the survey, representing a mean density of 0.24 per 100 m². Most clams were found at Raffles Lighthouse, Singapore's ‘best’ reef. No juvenile clams were encountered. In the aquarium experiment, clam growth was significantly different among the three light treatments, with growth greatest in the ～50% ambient PAR treatment. Of the 144 transplanted clams, 116 (80.6%) were recovered after 7 months. All specimens had increased in size, with growth rates among reefs ranging from 3.3 mm month^?1 (SD=1.3 mm) to 4.8 mm month^?1 (SD=1.6 mm).
• 5. Results suggest that, despite high levels of sedimentation and turbidity on Singapore's reefs, giant clams can survive and grow well. Restocking efforts using maricultured clams may be effective in enhancing the dwindling local populations. It is not clear, however, whether a self‐sustaining community can be established as high sedimentation may hinder larval settlement and survival.

Copyright © 2007 John Wiley & Sons, Ltd.     

柔性铰链放大的叠堆式超磁致伸缩致动器建模与实验

为满足新型电液伺服阀的驱动要求,设计了柔性铰链放大的叠堆式超磁致伸缩致动器(FASGMA),建立了FASGMA输出位移模型,并进行了实验验证和分析。首先,根据传统GMA偏磁施加方式的特点和不足,采用永磁体和GMM棒交替排布的结构形式,设计了叠堆式超磁致伸缩致动器(SGMA),并利用柔性铰链机构放大其输出位移;然后,根据SGMA的结构特点,建立了反映轴向分布不均匀性的SGMA应变模型;接着,利用力学基本原理和有限元法对柔性铰链机构的放大比和固有频率进行了分析,提出了结构优化设计的方法,完成了放大机构结构参数的确定;在此基础上,考虑SGMA与放大机构的相互作用以及SGMA轴向应变分布规律,建立了FASGMA多自由度位移模型,确定了自由度的合理取值;最后,搭建了FASGMA测试系统,进行了阶跃和正弦激励实验,完成了模型验证。结果表明:实验与模型计算结果吻合,证明了模型准确性;在阶跃激励下,FASGMA最大位移约为130μm,响应时间约为70 ms;正弦激励下,FASGMA工作频带为60 Hz,对激励信号有较好的跟随特性。