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1.
为了进一步研究甜菜谷胱甘肽转移酶BvGSTU9 (LOC104894060)在重金属胁迫过程中的功能。本研究以‘780016B/12优’为实验材料,对该基因序列特征、结构、功能进行预测分析,并利用qPCR检测该基因在不同浓度镉胁迫下的表达量变化。结果显示甜菜BvGSTU9基因全长925 bp,开放阅读框675 bp,编码了由224个氨基酸组成的不稳定膜外蛋白。BvGSTU9与菠菜、藜麦的氨基酸序列相似性较高,与系统发育进化树分析结果基本相符。二级和三级结构预测表明该基因主要由α-螺旋、β-折叠、延伸链及无规则卷曲组成。qPCR显示BvGSTU9基因在不同浓度的镉胁迫下均受到不同程度的诱导,因此可以推断甜菜BvGSTU9基因无论从结构还是功能上,与镉逆境胁迫存在着一定的应答关系。研究结果也为甜菜耐重金属镉机制研究提供参考依据。 相似文献
2.
Currently, very few aquaculture operations are employing airlift pump technology for water recirculation, aeration, and waste removal. This is likely due to the poor design and lower efficiency of traditional airlift design, the limited amount of research effort that has been invested in improving performance capabilities of air lift pumps and the general lack of awareness of the industry about the inherent advantages of airlift systems. A new efficient airlift pump is hydrodynamically designed by incorporating the Volume of Fluid (VOF) multi-phase model along with the K-ε turbulence model utilizing Computational Fluid Dynamics (CFD) tools. The pump is designed to offer a substantial reduction in total energy usage as well as an improved quality of the culture products in order to make it attractive to aquaculture industry. In this study, both numerical and experimental investigations were carried out for airlift systems operating under two different submergence ratios of 50% and 90% in a lab setting using 2.54 cm diameter pumps. Also, the performance of a large-scale pumps of 10.16 cm diameters were also tested in an aquaculture raceway to determine its effect on the operation. The numerical results were found to be in agreement with the experiments within ±20% which is considered very reasonable for multiphase flow analysis. The present study was found to present a great tool for modelling the airlift pump performance, and potentially proposing new designs. 相似文献
3.
【目的】得到含刚性沉水植物明渠的水流结构。【方法】采用粒子图像测速仪(PIV),用有机玻璃棒模拟刚性植物,在不同来水流量、植物密度条件下对明渠水流结构进行了试验研究。【结果】无植物时平均流速沿垂向呈对数分布规律,有植物时则呈现明显的分区分布特性。无植物时紊动强度值沿垂向变化不大,有植物时在植物顶端位置处紊动最剧烈。流量或植物密度越大,植物顶端位置的流速梯度就越大,植物层上方的流速最大值也越大。植物密度越大,植物顶端位置的紊动强度越大,植物密度对水流紊动强度由最大值减小到最小值的区域影响很大。【结论】刚性沉水植物的存在会改变水流结构,增强紊动掺混,增强流体质点交换和能量传递,且上述影响会随着流量或植物密度的增大而增强。 相似文献
4.
This paper evaluates the perception of crowding, its determinants and relationship with trail users’ experience in a peri-urban nature park. Existing crowding surveys rely heavily on a single-item 9-point crowding scale which was originally developed for backcountry areas. Receiver operating characteristic (ROC) analysis was used to test the validity and reliability of this measure in detecting crowding issues and formulating visitor capacity limits. Low validity and reliability raised concerns about the applicability of this scale in similar frontcountry areas. Inability of this simple crowding measure to detect a visitor threshold brings to the fore its another important limitation – ignorance of the complexity of crowding phenomenon. The perception of crowding varies with various social, psychological and situational factors, and a best-worst scaling (BWS) experiment was designed to simultaneously evaluate different aspects of crowding in the Medvednica Nature Park, a peri-urban nature park near Zagreb, Croatia. The findings of BWS experiment revealed that occupancy of mountain huts, number of other hikers on the trail and amount of litter along the trail on average contributed most to the trail users’ sense of crowding. A latent class analysis identified a large heterogeneity in the perception of crowding; while one group of trail users (63%) was more disturbed by the direct social impacts, the others (37%) were more disturbed by the secondary impacts of other visitors, especially by the negative externalities from road traffic in the park. Older visitors had a greater tendency to primarily associate crowding with the environmental conditions. A higher impact of trail use level in the perception of crowding, younger age and more frequent use were associated with a more negative evaluation of crowding. The park management should closely monitor road traffic in the park and visitor activity on highly used trails, especially on those popular among younger trail users. 相似文献
5.
李梅林 《湖南农业大学学报(自然科学版)》1990,17(3)
本文以内燃机排气噪声必需的消声量频率特性作为设计消声器的依据,论述了扩张室式和穿孔板复合消声器的设计.试验表明,采用这种新型复合消声器后,S195柴油机排气噪声得到了有效控制. 相似文献
6.
7.
Abstract – The reproductive biology of three species of rainbowfish (Melanotaeniidae) in northeastern Australian rainforest streams was investigated. Two species, Melanotaenia eachamensis and Cairnsichthys rhombosomoides are endemic to the area, whereas the third, M. splendida splendida, is more widespread. The species were all highly fecund, producing many hundreds of eggs between 1.10 and 1.24 mm in diameter. Melanotaenia eachamensis was the most fecund, produced the largest eggs of the three species, and consequently exhibited the greatest maternal investment (as measured by gonadosomatic index). The majority of reproductive effort occurred during the dry season, although reproductively active fish were present year-round for each of the species, but particularly so for M. s. splendida and C. rhombosomoides. No evidence for a role by temperature or photoperiod as environmental cues for reproduction was found, and it was suggested that gonad development was strongly tied to somatic growth. The concentration of reproduction to the dry season ensures that larvae are produced during a period of relatively stable and benign physical conditions. Comparison of temporal changes in gonadosomatic index values suggest that the spawning season of M. eachamensis , which occurs in high-elevation streams, is more restricted and commences about 1 month earlier than either other species. A similar phenology was observed for the M. s. splendida population found at high elevation and highlights the potential for spatial differences in stream productivity to influence life history. Note 相似文献
8.
Mid-storage hydration-dehydration treatments with medium-vigour lettuce seed very effectively reduced physiological deterioration in storage and ultimately showed better field emergence and final yield. Soaking-drying treatment of high-vigour lettuce seed was not effective. Pre-sowing treatment of medium-vigour lettuce seed, though less effective than mid-storage treatment, gave better field performance, but high-vigour seed did not benefit from the treatment. For carrot, both mid-storage and pre-sowing seed treatments proved beneficial, with the former showing better performance than the latter. Unlike lettuce, well-preserved carrot seed responded positively to the pre-sowing treatment. 相似文献
9.
在北京地区蟋蟀草从5月开始发生,可以持续到10月底,6月、7月出土速率和出土率强于其它月份,9月后出土率显著下降;5~10月期间生长的蟋蟀草,其种子与植株的生物学产量比率为5~7的值占64%。脱离母株的蟋蟀草种子当月存在萌发力,半年后萌发率达到60%。 相似文献
10.