Regulatory role of l-proline in fetal pig growth and intestinal epithelial cell proliferation

l-proline (Pro) is a precursor of ornithine, which is converted into polyamines via ornithine decarboxylase (ODC). Polyamines plays a key role in the proliferation of intestinal epithelial cells. The study investigated the effect of Pro on polyamine metabolism and cell proliferation on porcine enterocytes in vivo and in vitro. Twenty-four Huanjiang mini-pigs were randomly assigned into 1 of 3 groups and fed a basal diet that contained 0.77% alanine (Ala, iso-nitrogenous control), 1% Pro or 1% Pro + 0.0167% α-difluoromethylornithine (DFMO) from d 15 to 70 of gestation. The fetal body weight and number of fetuses per litter were determined, and the small and large intestines were obtained on d 70 ± 1.78 of gestation. The in vitro study was performed in intestinal porcine epithelial (IPEC-J2) cells cultured in Dulbecco''s modified Eagle medium-high glucose (DMEM-H) containing 0 μmol/L Pro, 400 μmol/L Pro, or 400 μmol/L Pro + 10 mmol/L DFMO for 4 d. The results showed that maternal dietary supplementation with 1% Pro increased fetal weight; the protein and DNA concentrations of the fetal small intestine; and mRNA levels for potassium voltage-gated channel, shaker-related subfamily, member 1 (Kv1.1) in the fetal small and large intestines (P < 0.05). Supplementing Pro to either gilts or IPEC-J2 cells increased ODC protein abundances and polyamine concentrations in the fetal intestines and IPEC-J2 cells (P < 0.05). In comparison with the Pro group, the combined administration of Pro and DFMO reduced the expression of ODC protein and spermine concentration in the fetal intestine, as well as the concentrations of putrescine, spermidine and spermine in IPEC-J2 cells (P < 0.05). Meanwhile, the percentage of cells in the S-phase and the mRNA levels of proto-oncogenes c-fos and c-myc were increased in response to Pro supplementation, whereas depletion of cellular polyamines with DFMO increased tumor protein p53 (p53) mRNA levels (P < 0.05). Taken together, dietary supplementation with Pro improved fetal pig growth and intestinal epithelial cell proliferation via enhancing polyamine synthesis.     

Influence of soil phosphorus status,texture, pH and metal content on the efficacy of amendments to pig slurry in reducing phosphorus losses

Cost‐effective strategies for using chemically amended organic fertilizers need to be developed to minimize nutrient losses in surface and groundwater. Coupling specific soil physical and chemical characteristics with amendment type could increase their effectiveness. This study investigated how water‐extractable phosphorus (P) was affected by chemical amendments added to pig slurry and how this effect varied with soil properties. A 3‐month incubation study was conducted on 18 different mineral soils, stored at 10 °C and 75% humidity and treated with unamended and amended slurry which was incorporated at a rate equivalent to 19 kg total P (TP )/ha. The amendments examined were commercial‐grade liquid alum, applied at a rate of 0.88:1 [Al:TP ], and commercial‐grade liquid poly‐aluminium chloride (PAC ), applied at a rate of 0.72:1 [Al:TP ]. These amendments were previously identified by the authors as being effective in reducing incidental losses of P. The efficacy of the amendments varied with the soil test P, the degree of P saturation (DPS ) and the Mehlich aluminium, iron and calcium, but not soil texture. Chemical amendments were most effective in soils with DPS over approximately 20%. Due to their high cost, the incorporation of amendments into existing management practices can only be justified as part of a holistic management plan where soils have high DPS .     

MapInfo在基准地价评估成果图中的应用实践

本文以MapInfo7.5作为GIS的平台软件,将MapInfo用于基准地价评估成果图的信息管理,利用创建、修改或更新的相关表格,建立相应的专题地图,使成果图中的信息更加直观明了。实践证明,MapInfo为基准地价评估成果图的及时更新、科学管理、有效利用提供了可靠的途经。     

中国水果供给结构性变化的实证研究

为分析中国水果供给的结构性变化，本文构造中国水果的供给模型，将1978-2001年24年划分为两个时期，利用Chow检验分别回归后发现我国水果供给的各种弹性均发生了明显的结构性变化，从而得出果农对价格的反应能力和敏感性增强、对农资投入的依赖性增强等重要结论。     

增瘦剂对长×巴猪(F_1)染色体诱变的研究

本文采用外局血淋巴细胞培养及姊妹染色体分染技术,对埋植增瘦剂的长×巴猪(F_1)染色体进行了研究,结果表明,在所统计的细胞中,试验组正常二倍体细胞(2n=3.8)的百分率为71.43％,对照组为80.36％,差异极显著(P<0.01);试验组多倍体细胞数(8.33％)极显著地高于对照组(2.23％,P<0.01);试验组染色体断裂细胞百分率(10.67％)与对照组(8.00％)之间差异不显著(P>0.05);试验组姊妹染色体交换频率(1.88)极显著地高于对照组(0.76,P<0.01)。     

猪雌激素受体基因研究进展

雌激素受体(ESR)是一种核酸受体，具有转录调控蛋白质的功能，影响着雌激素基因在雌性脊椎动物组织的表达，从而对第二性征、繁殖周期、生殖力、妊娠维持等方面产生影响。本文对雌激素受体的分布、生物学作用、分子结构、基因定位、多态性及其与猪繁殖性能的关系等方面作一综述。     

我国价格体系改革趋势初探

本文以我国国民经济系统与河南省经济子系统的同构性为基础,利用河南省1987年投入产出表,计算出诺依曼最优生产强度和与之相应的最优价格,进而对照现行价格,提出了我国价格体系改革走向的基本构想,并对其合理性、可行性进行了初步研究。     

美国黑豚养殖技术

美国黑豚属野生皮毛兽种类,是食草哺乳动物,形态小而肥胖,通身黑色与兔十分相似,成龄黑豚体重一般在800g左右,最重可达到1500-2000g,前肢短小,不善跳跃,行动敏捷。皮毛丰厚细密、黑亮光涌,是加工高档裘皮服装和工艺饰品的加工原料,豚肉是味道鲜美的上等佳品,其血液是提取歧氧化酶的最理想原料。具有很高的经济值和利用价值。     

泰地罗新注射液在藏香猪体内的药物动力学研究

为研究泰地罗新在藏香猪体内的药物动力学特征,了解其在藏香猪体内的吸收、分布、转化及排泄规律,为泰地罗新注射液的临床合理用药提供参考。本试验选取8头藏香猪,泰地罗新单剂量注射给药,不同时间点采集藏香猪血液,利用高效液相色谱法测定血浆中药物浓度。结果表明：泰地罗新注射给药(4 mg/kg.bw)后,泰地罗新在藏香猪体内的药-时曲线符合有吸收三室开放模型,其主要药动学参数为：Tmax为0.457±0.143h,Cmax为0.759±0.192 μg/mL,t1/2为120.518±10.181h,AUC为22.224±2.641μg.h/mL。表明泰地罗新注射给药后,在藏香猪体内吸收迅速,消除较为缓慢。     

Lipid-rich bovine serum albumin improves the viability and hatching ability of porcine blastocysts produced in vitro

The effects of lipid-rich bovine serum albumin (LR-BSA) on the development of porcine blastocysts produced in vitro were examined. Addition of 0.5 to 5 mg/ml LR-BSA to porcine blastocyst medium (PBM) from Day 5 (Day 0 = in vitro fertilization) significantly increased the hatching rates of blastocysts on Day 7 and the total cell numbers in Day-7 blastocysts. When Day-5 blastocysts were cultured with PBM alone, PBM containing LR-BSA, recombinant human serum albumin or fatty acid-free BSA, addition of LR-BSA significantly enhanced hatching rates and the cell number in blastocysts that survived compared with other treatments. The diameter, ATP content and numbers of both inner cell mass and total cells in Day-6 and Day-7 blastocysts cultured with PBM containing LR-BSA were significantly higher than in blastocysts cultured with PBM alone, whereas LR-BSA had no effect on mitochondrial membrane potential. The mRNA levels of enzymes involved in fatty acid metabolism and β-oxidation (ACSL1, ACSL3, CPT1, CPT2 and KAT) in Day-7 blastocysts were significantly upregulated by the addition of LR-BSA. The results indicated that LR-BSA enhanced hatching ability and quality of porcine blastocysts produced in vitro, as determined by ATP content, blastocyst diameter and expression levels of the specific genes, suggesting that the stimulatory effects of LR-BSA arise from lipids bound to albumin.