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L.Scott Cram Jean C Forslund Jerald L Jarnagin Charles O Thoen 《Comparative immunology, microbiology and infectious diseases》1980,3(3):373-380
A combination of whole blood cultures, hypotonic fluorescent staining of nucleated cells, and flow cytometry resulted in a greatly improved technique for measuring lymphocyte blastogenesis. Sample preparation was reduced to two steps with no centrifugation. The technique was successfully used to analyze blood cultures derived from experimentally and naturally infected cows. The technique was also shown to be ideally suited for analyzing lymphocytes cultured in whole blood cultures and (1) avoids the problems of tritiated thymidine analysis and (2) provides a direct analysis of the number of cells responding to antigenic stimulation. 相似文献
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为了快速、准确、方便地测量轮胎的附着力,设计了一种新的试验装置,测试结果表明,该装置结构简单、使用方便、测试精度较高,可直接在田间或路面上测量出轮胎的一些附着性能参数。 相似文献
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