全文获取类型
收费全文 | 950篇 |
免费 | 66篇 |
国内免费 | 108篇 |
专业分类
林业 | 53篇 |
农学 | 89篇 |
基础科学 | 99篇 |
69篇 | |
综合类 | 316篇 |
农作物 | 45篇 |
水产渔业 | 109篇 |
畜牧兽医 | 227篇 |
园艺 | 79篇 |
植物保护 | 38篇 |
出版年
2024年 | 2篇 |
2023年 | 14篇 |
2022年 | 20篇 |
2021年 | 28篇 |
2020年 | 34篇 |
2019年 | 39篇 |
2018年 | 32篇 |
2017年 | 43篇 |
2016年 | 47篇 |
2015年 | 59篇 |
2014年 | 59篇 |
2013年 | 46篇 |
2012年 | 56篇 |
2011年 | 60篇 |
2010年 | 63篇 |
2009年 | 72篇 |
2008年 | 67篇 |
2007年 | 54篇 |
2006年 | 53篇 |
2005年 | 44篇 |
2004年 | 33篇 |
2003年 | 26篇 |
2002年 | 18篇 |
2001年 | 12篇 |
2000年 | 16篇 |
1999年 | 11篇 |
1998年 | 19篇 |
1997年 | 23篇 |
1996年 | 12篇 |
1995年 | 17篇 |
1994年 | 6篇 |
1993年 | 6篇 |
1992年 | 4篇 |
1991年 | 6篇 |
1990年 | 9篇 |
1989年 | 5篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1982年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
排序方式: 共有1124条查询结果,搜索用时 15 毫秒
1.
Lactate Kinetics in veterinary Critical Care: A Review 总被引:3,自引:0,他引:3
Michael S. Lagutchik DVM Gregory K. Ogilvie DVM Wayne E. Wingfield MS DVM Timothy B. Hackett DVM MS 《Journal of Veterinary Emergency and Critical Care》1996,6(2):81-95.
Elevation in blood lactate concentration, with or without accompanying metabolic acidosis, is a hallmark finding in patients with circulatory compromise, and is also consistently noted in other conditions affecting critically ill or injured individuals. Little is reported in a veterinary literature regarding lactate measurement in the emergency and critical care setting, despite impressive reports of the clinical usefulness of lactate measurement in people. The purpose of this article is to review lactate kinetics and the clinical utility of lactate measurement. Limitations to lactate evaluation will also be discussed. 相似文献
2.
Improvements in human patient monitoring despite their development in animals, do not always find their way into veterinary clinical use due to financial constraints. Gastrointestinal intraluminal CO2 partial pressure (Gip1 CO2 ) monitoring, however, is not only proving very beneficial in human trauma and critical patient care but is also very likely to become relatively inexpensive. By providing information on the perfusion adequacy of a high risk, critically important tissue, the GI mucosa, GI P1 CO2 monitoring offers an easily accesible indicator of the efficacy and adequacy of resuscitative interventions. The potential for decreasing morbidity and mortality is enormous. Therefore, the practicing veterinarian should become familiar with GI P1 CO2 monitoring theory and technology so he or she can be better prepared to incorporate it into practice when in becomes available. 相似文献
3.
本文通过对9匹临床健康马激光麻醉实验研究,用18mWHe-Ne激光照射马胫神经30分钟,确认达到了良好的全身镇痛状态,观察分析了激光照射前后三个不同时期脑脊液(CSF)内乙酰胆碱(Ach)含量、真假胆碱酯酶活性的消长规律。激光照射30分后停照10分,CSF内Ach含量显著增高,即由照前的197.91±17.89pg/ml升高到405.50±23.45pg/ml,激光停照60分时,CSF内Ach含量恢复至与照前值没有显著差异的水平。认为在激光镇痛过程中中枢胆碱能神经元功能增强,积极参与了激光麻醉。正常马CSF中真性胆碱酯酶(AchE)与假性胆碱酯酶(ChE)活性分别为2.21±0.1454、0.36±0.0486μM/ml/37℃/30′。激光照射前后马CSF内AchE和ChE均未发生显著变化。 相似文献
4.
5.
Existing spatial patterns of a forest are in part a product of its disturbance history. Using laser altimetry and field measures
of canopy top height to represent pre- and post-hurricane canopy topography, respectively, we measured changes in spatial
patterns of stand structure of a United States southern mixed coniferous-deciduous for est. Autocorrelative and fractal properties
were measured in this opportunistic study to quantify changes in canopy architecture along twelve, 190-250 m transects that
were subjected to moderate to high levels of wind disturbance. Prior to the hurricane, canopy heights were autocorrelated
at scales <40 m with an average fractal dimension of 1.71. After the disturbance, autocorrelation disappeared; the average
fractal dimension rose to 1.94. This shift towards spatial randomness illustrates part of the cyclical nature of ecosystem
development. It shows how a catastrophic collapse of biomass accumulation corresponds to a decrease in ecosystem organization
across a landscape.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
6.
AIM:To study the relationship between myocardial HSP70 and PKC during myocardial ischemic preconditioning(IPC). METHODS: PKC inhibitor, polymyxin B(PMB) and PKC activator, phorbol 12-myristate 13-acetate(PMA) were applied to the models of myocardial ischemia/reperfusion in vivo and in vitro in rabbits, respectively, and the ventricular functions, PLA2 in the serum, and the expression of mycardial HSP70 mRNA were examined. RESULTS:IPC decreased PLA2 activity, improved the left ventricular function and increased the expression of myocardial HSP70 mRNA. Howerer, all these effects of IPC could be blocked by PMB. Interestingly, PMA mimicked IPC with attenuating the injuries of cardial myocytes and increasing myocardial HSP70 mRNA expression. CONCLUSION:PKC is involved in the myocardial HSP70 expression in case of ischemic preconditioning. 相似文献
7.
8.
A comparison of two sources of methionine supplemented at different levels on heat shock protein 70 expression and oxidative stress product of Peking ducks subjected to heat stress
下载免费PDF全文
![点击此处可从《Journal of animal physiology and animal nutrition》网站下载免费的PDF全文](/ch/ext_images/free.gif)
L. Guo R. Li Y. F. Zhang T. Y. Qin Q. S. Li X. X. Li Z. L. Qi 《Journal of animal physiology and animal nutrition》2018,102(1):e147-e154
9.
[目的]进一步了解茶树小分子量热激蛋白基因CsHSP17.2在逆境胁迫条件下的分子生物学功能.[方法]利用RT-PCR技术从茶树‘迎霜’中克隆得到CsHSP1 7.2基因,运用生物信息学软件分析其核苷酸和编码蛋白,通过Real-timePCR分析其表达模式.[结果]该基因开放阅读框长度为453 bp,编码150个氨基酸,蛋白质相对分子质量为17.2×10a,理论等电点5.56;无信号肽位点,属于非分泌型蛋白;被定位于细胞质中.系统发育树分析表明,茶树CsHSP1 7.2与水稻(GenBank登录号:P27777)和花生(ABC41131)的进化关系较近,属于小分子量热激蛋白基因家族第Ⅰ亚族.qRT-PCR分析发现,茶树CsHSP17.2属于组成型基因;高温(38℃)处理1h能显著提高CsHSP17.2 mRNA的相对表达量(P<0.05);干旱(100 g·L-1 PEG 6000)、高盐(200 mmol· L-1 NaCl)和外源脱落酸(200 mg· L-1 ABA)处理条件下,该基因的转录水平均出现不同程度的上调.[结论]克隆得到茶树‘迎霜’小分子量热激蛋白基因CsHSP17.2,其在花中表达量最高,且响应高温、干旱、高盐和外源脱落酸胁迫. 相似文献
10.