Effect of cocaine on caspase-3 in myocardiac cells of male rats in different age

AIM: To study the effect of cocaine on caspase-3 in myocardiac cells of male rats in different age. METHODS: Three-week-old(n=16), six-week-old(n=16) and twelve-week-old (n=16) male Sprague-Dawley rats were all divided into control groups and experiment groups randomly, each group had eight animals, experiment groups were given cocaine hydrochloride (15 mg·kg^-1 body weight) subcutaneously daily for four weeks. The ratio of heart weight to body weight (HW/BW, mg/g) were measured. DNA fragmentation of myocardia cells was determined by gel electrophoresis, and caspase-3 activity in myocardia cells was tested by flow cytometry (FCM). RESULTS: In three experiment groups, the DNA isolated from myocardial cells displayed clear ladder pattern. The HW/BW and the caspase-3 activity were increased significantly than those of control groups (P<0.01). CONCLUSIONS: Cocaine induced apoptosis in rat myocardial cells. The increase in caspase-3 activity may be the one of important pathways related to the induction of apoptosis.     

Relationship between expression of somatostatin and DNA ploidy in breast cancer

AIM: To investigate the relationship between somatostatin and the pathologic type, estrogen receptor,DNA ploidy of nuclei in tumor cells of breast cancer.METHODS: 67 cases of primary breast cancer and 25 cases of benign breast tumor were examined by immunohistochemical stretomyces avidin peroxidase method. 26 cases of breast cancer selected at random were analyzed by flow cytometry.RESULTS: Somatostatin expressed significantly higher in low malignant breast cancer than that in high malignant breast cancer (P<0.05). Most of cancers with positive staining of somatostatin were diploidy,most of cancers with negative staining were aneuploidy,there had significant difference between two groups (P<0.05).CONCLUSION: Somatostatin may delay the progress of breast cancer,and somatostatin levels in cancer tissues may become a useful indicator for assessing prognosis of patients with breast cancer.     

春麦田除草剂的应用与杂草群落演替

作者采用定点定位试验,对春麦田除草剂应用与杂草群落演替趋势进行研究。结果表明:在杂草群落多样性麦田,小麦连作并分别连续5年施用同一种除草剂,原杂草群落中占优势的靶标杂草得以控制,而非靶标杂草和抗、耐药性杂草因失去竞争和制约对象而猖獗发展,发生量较原来增加几倍至几十倍,并形成优势种群,对小麦造成新的更严重的危害。一种除草剂在同田块连续施用4年,由于杂草群落演替,抗、耐药杂草兴起,除草效果显著下降而失去其使用意义。作者提出除草剂配套使用、轮用、混用等措施,并配合以合理的轮作制度,以减轻杂草群落长期受到单一的定向选择性压力。     

海河平原区棉花饲用黑麦复种方式及水肥利用效率分析

2011年10月-2014年10月连续3年在河北省任县安排了定位试验,通过饲用黑麦不同播期与棉花构建几种棉草复种茬口搭配方式,从产量、经济效益、水分利用效率和养分利用效率以及对土壤肥力影响等方面综合分析棉花饲用黑麦复种的可行性及复种方式。结果表明,棉花可与饲用黑麦复种,为降低饲用黑麦对棉花的影响,饲用黑麦可适当推迟到10月20日播种,次年4月底收获,效益可提高5470.1元·hm^-2。棉花饲用黑麦复种与棉花单作相比,平均水分利用率提高16.49%;水分经济利用效率提高56.54%;并且土壤有机质平均增长幅度提高5.71%,土壤含盐量平均降低幅度达到23.72%,土壤改良效果明显;是适用于该区域的高效复种模式。     

生物炭及其在稻田尾水净化中的应用研究进展

保障水稻稳定增产事关国家粮食安全。水中的农药、化肥、重金属等物质随稻田尾水流出，排入周边水域，易造成水体面源污染。生物炭具有原材料丰富、孔隙结构发达、比表面积大、离子交换能力强、成本效益高等优势，已被广泛应用于环境修复领域。生物炭经适当改性能增加吸附活性位，提高吸附性能。在促沉净化材料中添加生物炭或生物炭基材料，可增强稻田面源污染促沉净化装置效能，提高稻田尾水利用率，助推农业绿色发展。本文介绍了稻田尾水的特点、常见处理技术及治理意义，阐述了生物炭及生物炭基材料在稻田尾水净化中的应用，展望了稻田尾水防治工作待深入开展的方向，并提出了生物炭材料在参与废水处理上存在的问题，以期为促进生态循环农业发展提供参考。     

兰州引黄灌区玉米田杂草群落及生态位研究

对兰州引黄灌区玉米田杂草进行了研究,初步了解了其杂草种类和杂草种群的消长动态。主要杂草有打碗花、田旋花、藜、刺儿菜、荠菜、稗草、马齿苋、反枝苋、狗尾草。主要杂草的干重为藜、反枝苋、稗草分别列第1、第2和第3位。生态位的研究结果表明,藜的时间、水平、垂直生态位宽度值最高,分别为0.952 6,0.971 8,0.937 9,其危害最大;其次为反枝苋和稗草。藜、反枝苋和稗草为优势种群,与玉米存在时间、水平、垂直空间的竞争激烈,并且这3种杂草的生态位重叠值也最大,因此在使用一种除草剂时,可能会导致另两种杂草的发生与蔓延。马齿苋尽管数量最多,但其在垂直方位的生态位宽度值最小,对玉米影响小,处于劣势。除草处理各经济性状指标值均较不除草对照高,其折合产量为10 111.11 kg/hm2,较不除草处理增产36.19%。     

野生老芒麦种质资源鉴定与基部叶鞘绒毛变异分析

为更好地利用老芒麦(Elymus sibiricus L.)野生资源,本研究从我国青藏高原、西北、华北、东北地区以及国外部分地区共采集了1 723份野生披碱草属种质资源,通过表型特征观测和流式细胞仪检测,共鉴定出了990份野生老芒麦种质,246份垂穗披碱草种质,并测得老芒麦的DNA含量在5.86～7.30 Gb之间,平均DNA含量为6.66 Gb;垂穗披碱草材料的DNA含量在9.50～10.36 Gb之间,平均DNA含量为9.97 Gb。研究结果显示,在西北、华北以及东北地区的部分野生老芒麦材料在苗期基部叶鞘出现了绒毛,分析发现该表型出现的概率与经度和纬度呈极显著正相关关系(P<0.01),与海拔、年年均气温以及年平均降雨量呈极显著负相关关系(P<0.01)。表明老芒麦苗期基部叶鞘绒毛的有无与环境因子之间存在着必然的联系,可能是老芒麦种质应对不同环境类型的适应性机制之一。本研究为老芒麦的形态鉴定提供了新的依据,为老芒麦种质资源挖掘和育种应用提供了材料基础。     

The Biological Effects of Low Frequency Electromagnetic Fields on Cells

The biological effects of ELFMES including proliferation and apoptosis of cells as well as the damage of DNA are initially evaluated with the treatment of plant cells and human pulmonary fibroblasts in low frequency magnetic fields with certain intensity and different time intervals in vitro. Cell culture, counting in vitro, fluorescent dyeing analysis and single cell gel electrophoresis are adopted. The results demonstrate that the electromagnetic field with the intensity of 3mT can affect the proliferation and apoptosis of cells significantly.     

Numerical Simulation of Flow field and Temperature field in Combustion Chamber Widened Suddenly

The flowfield and temperature field are numerically Simulated by the mathematical model of the fluid flow and heat transfer in circular combustion chamber widened suddenly. The distributions and influence factors of the flow field and temperature field are studied in it. The results have the guiding significance for the design of combustion chamber. The mathematical models can serve as the quantitative analysis methods for design.     

Calculation and Measurement of Electric Field along Polluted Cylindrical Insulator

In this paper,the electrical field distribution along the polluted cylindrical insu-lator before a nd after the local a re appea rs is calculated with the optim lzed pa rtially cha rge simula-tinn method and measured with the pockels device. Based on the calculating and measuring results,the in fluen ce of the electric field distri bu tion on the propagation of local a re in the flashover ofcyIlndrical insufator under various conditions is analyzed systematieally.