‘丰香’草莓叶片高效再生体系的建立

 以草莓主栽品种丰香( Fragaria ×ananassa Duch ‘Toyonoka’) 叶片为外植体, 研究了影响组织培养的多个因素, 建立了高效再生体系。结果表明: MS + TDZ 1.5 mg·L ^{- 1} + IBA 0.4 mg·L ^{- 1}培养基最适于不定芽分化。不同滤光膜(绿膜、红膜、蓝膜、黄膜) 对草莓不定芽的分化具显著效应, 绿膜和红膜对芽的分化有明显促进作用, 不定芽再生率达95%以上, 平均每个外植体再生芽数在25个以上; 而蓝膜和黄膜则不利于芽的分化。不同滤光膜光谱差异主要集中在300～700 nm, 红、绿膜在该波段光强较弱,而黄、蓝膜和荧光灯较强。暗处理4周比1、2、3周更有利于提高叶片的不定芽再生率, 此后转到光下培养, 获得的不定芽再生率可达100%。     

荔枝冷害过程中果皮色泽、花色素苷和类黄酮含量的变化

 研究了‘糯米糍’荔枝果实在3 ℃和0 ℃条件下果皮的色泽和花色素苷及类黄酮含量的变化。结果表明, 果实在0 ℃下14 d 时褐变指数迅速增大, 21 d 时已遭受不可逆的冷害。在冷藏过程中, 果皮的色泽指标a、L、C 值及花色素苷、类黄酮、总酚含量均随贮藏时间的延长而下降, 0 ℃下比3 ℃下降快。0 ℃下14～21 d , a、L、C 值分别下降了27.95 %、33.58 %和58.47 % , 而21 d 时果皮花色素苷、类黄酮和总酚含量依次为采收时的18.08 %、35.99 %和41.64 %。说明冷害加速了荔枝果皮花色素苷的降解及类黄酮和总酚的变化, 促进了褐变进程的发生。果皮a、L、C 值与褐变指数、花色素苷含量呈显著相关性( P < 0.05) ,采用自动测色色度计可真实地反映果皮花色素苷含量和褐变程度。     

Seasonal variation in pigmentation and anthocyanidin phenetics in commercial Eustoma flowers

The seasonal change in petal color and pigmentation of 29 commercial Eustoma cultivars was studied. The flowers are basically divided into four groups according to the major anthocyanidin phenotype in association with petal coloration, i.e., delphinidin (Dp)-based (purple flower), cyanidin (Cy)-based (reddish purple flower), pelargonidin (Pg)-based (pink flower), and none (white flower) groups. The constitution of petal anthocyanidins was not changed by forcing treatment in most of the flowers. Lightness (L^*) and chroma (C^*, color saturation) showed a change along with the increase/decrease of hue angle difference (ΔH^*), thus simultaneously the chromatic tonalities tended to move to redder and bluer, respectively. Floral pigment clustering described two flower groups in a dendrogram, based on anthocyanidin constitutions as phenetic markers, which are apparently the Dp- and Pg-based phenotypes of anthocyanidin syntheses. The Cy-based flowers made a subcluster with the Pg-based flowers, indicating a close relationship in the biosynthesis of the two anthocyanidins, and suggesting the Dp- and Pg-syntheses complement one another.     

基于纹理和位置特征的麦田杂草识别方法

以化学防除适期麦田杂草为研究对象，对利用条播作物的位置和纹理特征识别田间杂草的方法进行了研究。根据条播作物小麦作物行的间距相对固定等位置特征，利用植物像素直方图法确定作物行的中心线和行宽，并识别行间杂草。然后，以作物行中心为基准来选取纹理块，计算量化级数为8级的H颜色空间的共生矩阵，提取5个纹理特征参数，利用K均值聚类法判别分析各块的类别来识别行内杂草。研究结果表明，杂草的正确识别率约为93％，作物的错误识别率约为7％。     

离心泵整体叶轮流道数控加工误差的仿真

对数控加工所获得的叶轮流道的加工误差进行仿真。首先对刀位轨迹线进行归一化处理,以此计算出脊棱高度曲线,再采用线性插值的方法得到曲面上采样点的加工误差。由于自由曲面加工中的刀位点比较密集,本文利用彩色云图将加工误差可视化,完善了对数控加工结果的计算机几何仿真。该算法已在自行研制的叶轮CAM系统中得到验证,并且与实际加工结果一致。     

基于计算机视觉和神经网络的牛肉颜色自动分

将采集的牛胴体眼肌切面图像人工确定其颜色等级,然后通过计算机图像处理方法,分割出肌肉区域并提取出其在RGB和HIS颜色空间的颜色特征参数.设计一个以牛肉的颜色特征参数为输入、牛肉的颜色等级为输出的BP神经网络模型,通过训练,确定模型的结构参数,用测试样本对该模型进行验证.结果显示,用该模型进行牛肉颜色等级预测的正确率可达95%,耗时仅0.25s.表明利用所设计的模型可以对牛肉的颜色等级进行快速、准确的自动判定.     

基于计算机视觉的新疆棉种颜色分选系统设计

设计了一套基于机器视觉的脱绒棉种在线分选系统,采用种子平抛和气吹分离的方式实现了新疆地区红棕色棉种与黑色棉种的自动分选。研究了无序状态下的种子图像采集方法,并通过区域细分的方式解决了无序种子与气流喷嘴的对应关系。提出了种子位置跟踪和分离算法,实现了对棉种图像处理结果的延时分离操作。实验结果表明:在传送带速度为0.50 m/s时,分选精度为88.6%,选出率为80.7%。     

光质对彩色甜椒幼苗生长及酶活性影响

探讨了不同光质(白光、红光、黄光、绿光和蓝光)对不同品种彩色甜椒幼苗生长及酶活性的影响。结果表明,白光和黄光培养壮苗的效果最好,蓝光和红光次之,绿光下徒长。幼苗生长过程中,白光下叶片的过氧化物酶(POD)、过氧化氢酶(CAT)、硝酸还原酶(NR)和抗坏血酸酶(APx)活性最高,超氧化物氧化酶(SOD)活性最低,绿光下SOD活性最高。各种光质间存在差异性,白光较好。     

Effects of fruit bagging on coloring and related physiology,and qualities of red Chinese sand pears during fruit maturation

Red Chinese sand pears (Pyrus pyrifolia Nakai) are particular to China. In order to determine the effects of fruit bagging treatments (including bag types, bag removal patterns and dates) on fruit qualities and to understand the mechanism of coloring of red Chinese sand pears, two experiments were carried out. In the first experiment, fruit of ‘Meirensu’ were firstly covered by light-impermeable paper bags with different levels of light permeable liners during their early development stage, then, the whole bag were/not removed or only the outer layer of bags were removed 3, 2 or 1 weeks before harvest. Thus, the fruit were/not totally re-exposed or were under different levels of sunlight transmission (80.31% or 34.71%). Non-bagged fruit were used as the control. Bagging treatments significantly affected the concentration of anthocyanin and the visual qualities of pear fruit. Compared to control, fruit re-exposed totally for 2 or 3 weeks accumulated the largest amount of anthocyanin and fruit receiving 80.31% and 34.71% of sunlight for 1–3 weeks could synthesize a little anthocyanin, indicating that high light intensity is imperative for coloring in red Chinese sand pears. Bagging treatments did not affect contents of total soluble sugars, but decreased organic acids contents in fruit. In the second experiment, fruit of ‘Meirensu’ and ‘Yunhongli No. 1’ were covered with only one type of light-impermeable bag during the early development stage and totally re-exposed after the bag removal 15 days before harvest. Fruit were then collected at different intervals to trace the time-course of coloring, and related physiology and inner qualities. With increasing time after the bag removal, the concentration of chlorophyll, carotenoid, flavonoid and total phenols changed little, but the concentration of anthocyanin accumulated extremely fast within 10 days after the bag removal in both cultivars and thereafter kept constant. ‘Yunhongli No. 1’ had higher anthocyanin contents and lower hue angle than ‘Meirensu’, indicating a higher potential of anthocyanin synthesis. After the bag removal, the sucrose contents and PAL activities increased gradually and correlation analysis revealed that they were highly correlated with anthocyanin accumulation in two cultivars. This study suggests that anthocyanin biosynthesis in red Chinese sand pears is a highly light dependent process and modified by genotypes. Based on the current results, in order to obtain red Chinese sand pear fruit with attractive appearance and good inner qualities, fruit must be covered with light-impermeable bags at the early stage of fruit development and the bag should be removed totally at least 10 days before harvest.     

驾驶员的视觉特性对道路交通安全的影响研究

为了更好地提高道路交通安全管理水平、减少交通事故,本文对关系驾驶员安全行车的视觉特性如视力、视野、立体视觉、色视觉、视觉适应等因素结合交通事故统计资料进行了分析,阐述了驾驶员的视觉特性对道路交通安全的影响。