排序方式: 共有29条查询结果,搜索用时 31 毫秒
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建立高效液相色谱法(HPLC)测定复方非泼罗尼滴剂中抗氧剂叔丁基对羟基茴香醚(BHA)与二丁基羟基甲苯(BHT)含量。采用高效液相色谱法,色谱柱为Agilent Zorbax SB-C18(250×4.6 mm, 5μm),流动相A为乙腈-甲醇-水(47∶21∶32),混合前在水中加入1%冰醋酸,流动相B为乙腈,线性梯度洗脱;检测波长为285 nm;流速为0.9 mL/min;柱温为30℃;进样量为20μL;采用外标法计算BHA、BHT含量。在建立的色谱条件下,空白溶液、阴性样品对BHA、BHT的测定均无干扰;BHA、BHT的检测限分别为0.08μg/mL和0.19μg/mL,定量限分别为0.31μg/mL和0.77μg/mL,分别在5.13~15.39μg/mL与2.56~7.66μg/mL范围内线性关系良好;平均回收率分别为98.5%和99.9%(n=9)。所建立的方法简单易行、专属性强、准确度高、灵敏度好,可用于复方非泼罗尼滴剂中抗氧剂的含量测定。 相似文献
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BHT对苹果采后灰霉病的防效及防御酶活性和丙二醛含量的影响 总被引:1,自引:1,他引:0
为探究2,6-二叔丁基-4-甲基苯酚(2,6-di-tert-butyl-4-methylphenol,BHT)对苹果采后灰霉病的防效和防病机制,采用平板法和刺伤接种法测定了BHT对灰霉病菌Botrytis cinerea的抑制作用及果实内防御酶活性和丙二醛含量的影响。结果表明,0.1 mmol/L BHT对苹果灰霉病的防效最佳,处理苹果后间隔48~96 h接种灰霉病菌,其防效可达68.59%~73.55%,其次为0.2、1.0 mmol/L BHT处理,但防效均低于52.94%。0.1 mmol/L BHT处理对灰霉病菌菌丝生长和分生孢子萌发无显著抑制作用,但可显著增强果实内超氧化物歧化酶、过氧化物酶和过氧化氢酶的活性以及总抗氧化能力,其峰值是对照的1.82~5.28倍,且均显著高于单独接种灰霉病菌的处理。此外,单独接种灰霉病菌的苹果果实内丙二醛含量快速升高,最大增幅达251.49%,而BHT处理后丙二醛含量变幅较小,最大增幅仅为83.95%。表明BHT可通过增强苹果果实内防御酶活性水平和总抗氧化能力来降低丙二醛的积累,从而提高果实对灰霉病的抗性。 相似文献
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为测定2,6-二叔丁基对甲酚(BHT)的抑菌活性,采用生长速率法、悬滴法分别测定了该物质对6种植物病原真菌菌丝生长及孢子萌发的抑制作用。结果表明:该物质对所测6种病原真菌均具有一定的抑制作用,抑制菌丝生长的EC50在129.18~1113.85 mg·L-1之间,其中对小麦赤霉病菌的抑制作用最强,EC50为129.18 mg·L-1;抑制孢子萌发的EC50在160.51~356.03 mg·L-1之间,其中对小麦叶枯的抑制率最高,EC50为160.51 mg·L-1。与4种常用药剂对小麦赤霉及番茄早疫病菌菌丝的抑制作用比较,BHT的抑制中浓度相对较大。 相似文献
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以还原能力和超氧阴离子抑制率为抗氧化指标,确定鹰嘴豆抗氧化多肽与其它抗氧化剂(Vc、VE及BHT)的协同抗氧化作用。结果表明:VE、Vc在低浓度时与鹰嘴豆抗氧化肽具有协同的还原能力,BHT与鹰嘴豆抗氧化肽未表现出协同还原能力;Vc、VE与鹰嘴豆抗氧化肽未显示出显著抑制超氧阴离子的协同效应,但BHT与鹰嘴豆抗氧化多肽表现出明显的抑制超氧阴离子的协同效应;且所有的协同作用随添加量和作用时间的增加而增强。 相似文献
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Raktim Sarmah Sarada Kanta Bhagabati Rajdeep Dutta Dipanka Nath Hemanta Pokhrel Lawonu P. Mudoi Nishi Sarmah Jiten Sarma Abdul M. Ahmed Rupam Jyoti Nath Lucy Ingtipi Kedolhouse Kuotsu 《Aquaculture Research》2020,51(9):3839-3846
The synthetic antioxidant 3,5‐di‐tert‐butyl‐4‐hydroxytoluene (BHT) is widely used as an additive in the food, cosmetic and plastic industries to increase the tenability of food and plastic for the past 70 years. BHT is degraded to 3,5‐di‐test‐butyl‐4‐hydroxybenzaldehyde (BHT‐CHO) in mammals, as well as in the natural environment such as in river and water. The average daily intake of BHT for human being is estimated to be 0.3 mg/kg body weight. Even though it is considered safe for human at authorized level, but its ubiquitous presence in the aquatic environment and the controversial toxicological data are of great concern for human as well as aquatic life. The experimental findings of zebrafish embryo toxicity test (ZFET) showed that the acute toxicity of 96‐hr (LC50) exposure during the embryogenic stage was found to be 4.388 mg/L and the effective concentration (EC50) was 1.375 mg/L. The reduce heart rate from the sublethal concentrations indicates the chemical to be cardiotoxic but a further review is to be needed. The Teratogenic Index (TI) calculated to be 3.19, which implies the compound may be a potential teratogen in aquatic life. The findings obtained in this study will stretch more evidence regarding developmental toxicity of BHT, which will be of much importance in further risk assessment of ecotoxicological studies. 相似文献
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分别在稀释、离心猪精液中添加0.2、0.4、0.8、1.6 mmol/L丁羟基甲苯(Butylated hydroxytoluene,BHT),15℃保存,检验保存35、d后精子TMS、PMI和NAR(%)、测定MDA浓度;确定并选择最佳BHT浓度用于猪精液冷冻保存,检验解冻后TMS、PMI、NAR和Mt-MP(%)、测定MDA浓度。结果显示:BHT显著提高稀释精液、离心精液各项指标百分率(P0.05),且MDA浓度显著降低(P0.05),BHT最佳浓度分别为0.8、1.6 mmol/L;0.8 mmol/L BHT显著提高冷冻-解冻精液各项指标百分率(P0.05),且MDA浓度显著降低(P0.05)。结果表明,BHT能通过抑制精子质膜氧化损伤,提高猪精液保存效果。 相似文献
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A. Sanches Silva J. M. Cruz Freire R. Franz P. Paseiro Losada 《Journal of Cereal Science》2008,48(3):662-669
Cereals are widely consumed foodstuffs and it is therefore important to take them into account when estimating consumer exposure to packaging-related chemicals. The mass transport of three model migrants (diphenylbutadiene, triclosan and BHT) from low-density polyethylene (LDPE) within dry foodstuffs of different particle size (rice and wheat flour) was studied because of the relationship between consumer exposure and possible harmful effects on human health. The conditions that most affect the mass transport of substances within dry foods are evaluated and discussed. The diffusion coefficients (DF) for diphenylbutadiene (DPBD) and triclosan in the studied foodstuffs were estimated. The DF values for DPBD and triclosan in wheat flour at 25 °C were 7.1 × 10−8 cm2/s and 3.3 × 10−8 cm2/s, respectively. The DF values for DPBD and triclosan in rice at 25 °C were 4.7 × 10−8 and 4.3 × 10−8 cm2/s, respectively. BHT displayed different behaviour and other tests were therefore carried out to elucidate the associated mass transport process. 相似文献